Experimental Glycoscience

Part 1. Glycosyltransferase Genes

1. Convenient Synthesis of Glycan-Related Oligosaccharides and Their Transformation into Neoglycoconjugates Using Enzymatic Methods
Takeomi Murata, Taichi Usui

2. Comparison of Glycosyltransferase Families Using the Profile Hidden Markov Model
Norihiro Kikuchi

3.
N-Acetylglucosaminyltransferases Involved in N-Glycan Biosynthesis
Kei-ichiro Inamori, Naoyuki Taniguchi

4. UDP-N-Acetyl-d-Galactosamine:polypeptide N-Acetylgalactosaminyltransferases (ppGaNTases, pp-GalNAc-Ts, EC 2.4.1.41)
Kouichi Tachibana

5. Glycosyltransferase Family with β4GT Motif (β4Gal-T and β4GalNAc-T Family)
Takashi Sato, Hisashi Narimatsu

6. β1,3-glycosyltransferase Gene Family and IGnT Gene Family
Akira Togayachi, Takashi Sato, Hisashi Narimatsu

7.
O-Mannosyltransferase and POMGnT1
Tamao Endo, Hiroshi Manya

8. Glycosyltransferases Involved in O-fucose Glycan Synthesis
Tetsuya Okajima, Tsukasa Matsuda

9. Fucosyltransferase (α1,2/ α1,3/ α1,4-fucosyltransferases)
Takashi Kudo, Hisashi Narimatsu

10. FUT8 Assay
Hideyuki Ihara, Hiroaki Korekane, Akio Matsumoto, Naoyuki Taniguchi

11. Sialyltransferases
Shuichi Tsuji

12. Glucuronyltransferases Involved in the HNK-1 Biosynthesis
Shinako Kakuda, Toshisuke Kawasaki, Shogo Oka

13. α1,4-Linkage Glycosyltransferases
Jun Nakayama, Koichi Furukawa

14. Glycosyltransferases Involved in the Biosynthesis of Glycolipids
Koichi Furukawa, Akiko Tsuchida, Keiko Furukawa

15. Sialyltransferases and Other Enzymes Involved in the Biosynthesis of Gangliosides
Koichi Furukawa, Keiko Furukawa

16. Heparan Sulfate Synthases and Related Genes
Shuji Mizumoto, Hiroshi Kitagawa

17. Chondroitin Sulfate Biosynthesis and Related Genes
Hideto Watanabe, Koji Kimata

18. Biosynthesis of Keratan Sulfate
Akira Seko, Katsuko Yamashita

19. Hyaluronan Synthase Assay
Naoki Itano

20. Glycosylphosphatidylinositol-Anchored Protein Biosynthesis and Related Genes in Mammalian Cells
Yusuke Maeda, Taroh Kinoshita

21. Cloning of Genes for N-linked Oligosaccharide Synthesis in Endoplasmic Reticulum
Ken-ichi Nakayama, Yoshifumi Jigami

22.
N-acetylglucosamine-6-O-sulfotransferases
Kenji Uchimura, Takashi Muramatsu

23. Sulfotransferases Involved in Sulfation of Glycosaminoglycans
Osami Habuchi

24. Sulfatide Synthase
Koichi Honke

25. CMP-N-acetylneuraminic Acid Hydroxylase
Akemi Suzuki

26. Nucleotide Sugar Transporter Genes and Their Functional Analysis
Shoko Nishihara

27. Processing Enzymes Involved in N-glycan Biosynthesis and Related Genes: the Golgi N-glycan Processing α-mannosidase II and α-mannosidase IIx
Tomoya O. Akama, Michiko N. Fukuda

28. Sialidase Genes
Taeko Miyagi

29. Release of Sugar Chains from Glycosphingolipids
Makoto Ito

30. Heparan Sulfate Endosulfatase Assay
Kazuko Keino-Masu, Masayuki Masu

31. Sphingolipid Activator Proteins
Junko Matsuda

32. Affinity Purification of Recombinant Galectins
Jun Iwaki, Jun Hirabayashi

33. Siglec Family
Takashi Angata

Part 2. Functional Analyses of Sugar Chains

34. NKT Cells and Their Recognition of Glycolipids
Ken-ichiro Seino, Masaru Taniguchi

35. Carbohydrate Ligands for Selectins in Immune Cell Trafficking
Reiji Kannagi, Katsuyuki Ohmori

36. Carbohydrate Recognition by Cytokines and Its Relevance to Their Physiological Activities
Keiko Fukushima, Katsuko Yamashita

37. Detection of Mouse MGL1/CD301a by a Specific Monoclonal Antibody
Kaori Denda-Nagai, Kiwamu Suzuki, Mitsuhiro Goda, Akihiko Kudo, Hayato Kawakami, Tatsuro Irimura

38. Roles of Serum Lectins in Host Defense
Nobuko Kawasaki, Bruce Yong Ma, Toshisuke Kawasaki

39. Siglec-2 Is a Key Molecule for Immune Response
Takeshi Tsubata

40. Galectin and Immune System: Toward Clinical Applications
Mitsuomi Hirashima

41. Novel Glycolipids in Developing Brain: Determination by Nano-LC/MS/MS
Yoshio Hirabayashi, Yasuko Nagatsuka, Shinya Ito

42. Functional Roles of the HNK-1 Carbohydrate and Polysialic Acid in the Nervous System
Yasuhiko Kizuka, Shogo Oka

43. Neuronal Function of Sulfatide
Koichi Honke

44. Accumulation of Alzheimer β-amyloid via Ganglioside Clusters
Katsumi Matsuzaki

45. Chondroitin Sulfate Proteoglycans and Neuronal Network Formation
Nobuaki Maeda

46. Processing of Glycosyltransferases by Alzheimer’s β-secretase (BACE1)
Shinobu Kitazume, Shou Takashima, Yasuhiro Hashimoto

47. Chondroitin Sulfate Proteoglycans: A Key Substance for Central Nervous System Injury Repair
Atsuhiko Oohira

48. A Cytoplasmic Peptide: N-Glycanase and ER-Associated Degradation
Tadashi Suzuki

49. Ubiquitination of Glycoproteins in the Cytosol
Tadashi Tai

50. Degradation of Misfolded Glycoproteins in the Endoplasmic Reticulum
Nobuko Hosokawa

51. Retrograde Transport of Glycolipid-Bound Toxins
Ryo Misaki, Tomohiko Taguchi

52. Degradation of Hyaluronan and Its Disorder
Masaki Yanagishita, Katarzyna Anna Podyma-Inoue

53. Recent Advances in Enzyme Replacement Therapy for Lysosomal Diseases
Kohji Itoh

54. Developing an Assay System for the Hemagglutinin Mutations Responsible for the Binding of Highly Pathogenic Avian Influenza A Viruses to Human-Type Receptors
Yasuo Suzuki

55.
Helicobacter pylori Growth Assay for Glycans
Jun Nakayama

56. Binding Properties of Clostridium botulinum Type C Progenitor Toxin
Atsushi Nishikawa

57. Fucosylation and Cancer
Eiji Miyoshi

58. Biological Significance of Mucins Produced by Epithelial Cancer Cells
Hiroshi Nakada

59. Regulation of Glycosyltransferases for O-Glycan Core Structure in Cancer and Their Relations to Metastasis
Toshie Iwai, Hisashi Narimatsu

60. Roles of Carbohydrate-Mediated Cell Adhesion in Cancer Progression
Reiji Kannagi, Akira Seko

61. Conditional Transgenic Mouse of Hyaluronan Synthase: A Potential Model of Advanced Breast Cancer
Naoki Itano

62. Proteoglycans in Tissue Regeneration
Koji Kimata

63. Stem Cell Glycobiology
Mitsuru Nakamura

64. Human Embryonic Stem (ES) Cells and Induced Pluripotent Stem (iPS) Cells Are Defined by Carbohydrate Markers
Akihiro Umezawa, Masashi Toyoda

65. Glycoantigens in Xenotransplantation
Shuji Miyagawa

66. Glycoconjugates in Spermatogenesis
Koichi Honke

67. Recognition Mechanism of Egg and Sperm Based on Sugar Chains
Midori Matsumoto

68. Functional Analysis of Sugar Chains Using a Genome-Wide RNAi System in Drosophila

Shoko Nishihara

69. Functional Glycomics at the Level of Single Cells: Studying Roles of Sugars in Cell Division, Differentiation, and Morphogenesis with 4D Microscopy
Souhei Mizuguchi, Katsufumi Dejima, Kazuko H. Nomura, Daisuke Murata, Kazuya Nomura

70. Studies on Functions of Notch O-Fucosylation in Drosophila

Tomonori Ayukawa, Kenji Matsuno

71. Membrane Microdomain as a Platform of Carbohydrate-Mediated Interactions During Early Development of Medaka Fish
Tomoko Adachi, Chihiro Sato, Ken Kitajima

72. Functions of Heparan Sulfate Proteoglycans in Morphogenesis
Hiroko Habuchi, Koji Kimata

73. Fukutin and Fukuyama Congenital Muscular Dystrophy
Motoi Kanagawa, Tatsushi Toda

74. Congenital Muscular Dystrophies Due to the Glycosylation Defect
Tamao Endo

75. Molecular Diagnosis of Congenital Disorders of Glycosylation
Yoshinao Wada

76. Research in Japan Has Contributed to the Understanding of GPI Anchor Deficiency
Yoshiko Murakami, Taroh Kinoshita

77. Aberrant Expression of Sialidase in Cancer and Diabetes
Taeko Miyagi

78. Insulin Resistance and Type 2 Diabetes as Microdomain Disease: Implication of Ganglioside GM3
Jin-ichi Inokuchi, Kazuya Kabayama

79. Incompleteness of O-glycans at the Hinge Region of IgA1 and IgA Nephropathy
Hitoo Iwase, Yoshiyuki Hiki

80. β4-Galactosyltransferase Deficiency and IgA Nephropathy
Masahide Asano

81. Glycosyltransferases Involved in the Biosynthesis of IgA O-Glycans
Yoshiki Narimatsu, Hisashi Narimatsu

82. Importance of Sugar Chains in the Function of Growth Factor Receptors
Yoshitaka Ikeda

83. Analysis of N-glycan of Growth Factor Receptors
Motoko Takahashi

84. The Midkine Family and Its Receptors
Kenji Kadomatsu

85.
N-glycans Regulate Integrin α5β1 Functions
Jianguo Gu, Yuya Sato, Tomoya Isaji

86. Blood Group Antigens: Blood Group Carbohydrate Antigens
Koichi Furukawa, Koichi Iwamura, Keiko Furukawa

87. Bombay, Lewis and Ii Blood Group Antigens on Human Erythrocytes
Hisashi Narimatsu

88. Blood Cells in Glycobiology
Mitsuru Nakamura

Part 3. Glycosyltransferase Gene KO Mice

89. Fucosyltransferase-9 Knockout Mouse
Takashi Kudo, Hisashi Narimatsu

90. Knockout Mice of α1,6 Fucosyltransferase (Fut 8)
Naoyuki Taniguchi

91. Glucuronyltransferase (GlcAT-P) Gene-Deficient Mice
Ippei Morita, Shogo Oka

92. KO Mice of β1,4-galactosyltransferase-I
Masahide Asano

93. Sulfotransferases
Takashi Muramatsu, Kenji Uchimura

94. KO Mice of Cerebroside Sulfotransferase
Koichi Honke

95. KO Mice of β1,4-N-acetylgalactosaminyltransferase (GM2/GD2 Synthase)
Koichi Furukawa, Kogo Takamiya, Keiko Furukawa

96. KO Mice of α-2,8-sialyltransferase (GD3 Synthase)
Koichi Furukawa, Masahiko Okada, Keiko Furukawa

97. Double KO Mice of β-1,4-N-acetylgalactosaminyltransferase (GM2/GD2 Synthase) and α-2,8-sialyltransferase (GD3 Synthase)
Koichi Furukawa, Orie Tajima, Yusuke Ohmi, Keiko Furukawa

Part 4. Infrastructures and Research Resources

98. Cell Lines for Glycobiology
Mitsuru Nakamura

99. Sugar Chain-Related Genes: Glycosyltransferases Expression System
Takashi Sato, Hisashi Narimatsu

100. GALAXY Database and Pyridylaminated Oligosaccharide Library
Koichi Kato, Noriko Takahashi

101. Mass Production of Sugar Intermediates for the Synthesis of Functional Oligosaccharides
Yuji Matsuzaki

102. A Database System for Glycogenes (GGDB)
Akira Togayachi, Kwon-Yeon Dae, Toshihide Shikanai, Hisashi Narimatsu

103. CabosDB: Carbohydrate Sequencing Database
Norihiro Kikuchi

104. GlycoEpitope: A Database of Carbohydrate Epitopes and Antibodies
Toshisuke Kawasaki, Hiromi Nakao, Tomoko Tominaga

105. A Novel Lectin-Affinity Database for Structural Glycomics
Yoriko Takahashi, Jun Hirabayashi

106. Glycoconjugate Data Bank
Nobuaki Miura, Shin-Ichiro Nishimura

107. KEGG GLYCAN for Integrated Analysis of Pathways, Genes, and Structures
Kosuke Hashimoto, Minoru Kanehisa

108. DNA Microarray in Glycobiology
Harumi Yamamoto, Hiromu Takematsu, Yasunori Kozutsumi

109. Lectin Microarray
Atsushi Kuno, Jun Hirabayashi

110. Carbohydrate Microarray for Deciphering the Information Embedded in Oligosaccharide Structures
Shigeyuki Fukui

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