Optical Fluorescence Microscopy

1. Fundamentals of Optical Microscopy
Franco Quercioli

2. The White Confocal: Continuous Spectral Tuning in Excitation and Emission
Rolf Borlinghaus

3. Second/Third Harmonic Generation Microscopy
Shakil Rehman, Naveen K. Balla, Elijah Y. Y. Seng, Colin J. R. Sheppard

4. Role of Scattering and Nonlinear Effects in the Illumination and the Photobleaching Distribution Profiles
Zeno Lavagnino, Francesca Cella Zanacchi, Alberto Diaspro

5. New Analytical Tools for Evaluation of Spherical Aberration in Optical Microscopy
Isabel Escobar, Emilio Sánchez-Ortiga, Genaro Saavedra, Manuel Martínez-Corral

6. Improving Image Formation by Pushing the Signal-to-Noise Ratio
Emiliano Ronzitti, Giuseppe Vicidomini, Francesca Cella Zanacchi, Alberto Diaspro

7. Site-Specific Labeling of Proteins in Living Cells Using Synthetic Fluorescent Dyes
Gertrude Bunt

8. Imaging Molecular Physiology in Cells Using FRET-Based Fluorescent Nanosensors
Fred S. Wouters

9. Measuring Molecular Dynamics by FRAP, FCS, and SPT
Kevin Braeckmans, Hendrik Deschout, Jo Demeester, Stefaan C. Smedt

10. In Vitro–In Vivo Fluctuation Spectroscopies
M. Collini, L. D’Alfonso, M. Caccia, L. Sironi, M. Panzica, G. Chirico, I. Rivolta, B. Lettiero, G. Miserocchi

11. Interference X-ray Diffraction from Single Muscle Cells Reveals the Molecular Basis of Muscle Braking
L. Fusi, E. Brunello, M. Reconditi, R. Elangovan, M. Linari, Y.-B. Sun, T. Narayanan, P. Panine, G. Piazzesi, M. Irving, V. Lombardi

12. Low Concentration Protein Detection Using Novel SERS Devices
Gobind Das, Francesco Gentile, Maria Laura Coluccio, G. Cojoc, Federico Mecarini, Francesco Angelis, Patrizio Candeloro, Carlo Liberale, Enzo Fabrizio

13. Near Infrared Three-Dimensional Nonlinear Optical Monitoring of Stem Cell Differentiation
Uday K. Tirlapur, Clarence Yapp

14. A Correlative Microscopy: A Combination of Light and Electron Microscopy
Umberto Fascio, Anna Sartori-Rupp

Nyckelord: Life Sciences, Biological Microscopy, Cell Biology, Biophysics and Biological Physics, Biochemistry, general