EMC 2008 14th European Microscopy Congress 1–5 September 2008, Aachen, Germany

L. Life Science

1. Cryoelectron microscopy: from molecules to systems
W. Baumeister

2. Progress in High-resolution Scanning Probe Microscopy
A. Engel, P. Bosshart, P. Frederix, S. A. Müller

L1. Analysis of macromolecules and their supramolecular assemblies

3. Modular organization of RNase P
Bettina Böttcher, Claire Batisse, Kyriaki Galani

4. Molecular anatomy of the human pathogen Leptospira interrogans

Martin Beck, Johan Malmström, Vinzenz Lange, Alexander Schmidt, Erich Deutsch, Ruedi Aebersold

5. Unveiling conformational changes of biological molecules using multiscale modeling and multiresolution experiments
Florence Tama

6. Nicolas Boisset: In memoriam
José L. Carrascosa

7. Cryo TEM-based 3D reconstruction of the recombinant expressed human zinc peptidase Meprin ?
Philipp Arnold, Arne Moeller, Frank Depoix, Christoph Becker-Pauly, Walter Stöcker, Ulrich Meissner

8. Origin melting by SV40 Large T antigen-new insights from 3D-EM-MLF3D classification method
J. M. Carazo, I. Cuesta, R. Nuñez-Ramirez, S. H. Scheres

9. Sequence analysis and modelling of the two large subunits of Phosphorylase Kinase
C. Carrière, J. -P. Mornon, S. Jonic, E. Larquet, C. Venien-Bryan, N. Boisset, I. Callebaut

10. Cellular Uptake of Polymer Nanoparticles Imaged by Electron Microscopy
M. Dass, A. Musyanovych, K. Landfester, P. Walther

11. 9 Å cryo-EM structure and molecular model of a gastropod hemocyanin didecamer (KLH1) reveals the architecture of the asymmetric collar
C. Gatsogiannis, K. Büchler, F. Depoix, J. Markl

12. Microstructure of model systems for sauces based in polysacharides observed by Cryo-SEM
I. Hernando, L. M. Guardeño, E. Llorca, A. Quiles, I. Pérez-Munuera

13. Three-dimensional architecture of outer- and inner-dynein arms in flagella revealed by cryo-electron tomography and single particle analysis
T. Ishikawa, K. H. Bui, T. Movassagh, H. Sakakibara, K. Oiwa

14. Structural and functional studies of rabbit skeletal muscle Phosphorylase Kinase
S. Jonic, C. Carrière, E. Larquet, V. Skamnaki, L. Johnson, C. Venien-Bryan, J. -P. Mornon, I. Callebaut, N. Boisset

15. Single-particle analysis of the Cdc6/Orc1, archaeal DNA replication initiator protein
N. Kajimura, H. Suzuki, K. Mayanagi, Y. Ishino, K. Morikawa

16. A high-throughput acquisition toolbox in Matlab for cryo-electron microscopy studies
A. Korinek, F. Beck, S. Nickell, W. Baumeister, J. M. Plitzko

17. Architecture of bacterial glutamate synthase complexe using hybrid approaches
M. Cottevieille, E. Larquet, S. Jonic, I. Callebaut, D. Svergun, M. A. Vanoni, N. Boisset

18. 3D Structure of DNA Repair Macromolecular Complexes Participating in Non-Homologous End-Joining (NHEJ)
O. Llorca, A. Rivera-Calzada, L. Spagnolo, M. A. Recuero-Checa, L. H. Pearl

19. Quaternary structure of recombinant human meprin ?, a zinc peptidase of the astacin family
Ulrich Meissner, Philipp Arnold, Arne Moeller, Frank Depoix, Christoph Becker-Pauly, Walter Stöcker

20. Allosterism of Nautilus pompilius hemocyanin as deduced from 8 Å cryo-EM structures obtained under oxy and deoxy conditions
A. Moeller, C. Gatsogiannis, F. Depoix, U. Meissner, J. Markl

21. High resolution structure of a 6 MDa protease by xray-crystallography and cryo-EM
B. Rockel, C. K. Chuang, G. Seyit, P. J. Walian, J. Peters, W. Baumeister, B. K. Jap

22. Cryo-TEM of liquid crystalline particles: Application of materials science techniques to study liquid crystalline particle structures
L. Sagalowicz

23. Structural basis for the concerted integration of HIV-1 DNA in the human genome, role of the human cofactor LEDGF/p75
P. Schultz, F. Michel, R. Benarous, M. Gottikh, D. Moras, M. Ruff

24. Titan Krios: Automated 3D imaging at ambient and cryogenic conditions
Marc Storms, Raymond Wagner, Uwe Luecken, Werner Hax, Matt Harris

25. Self assembly and phase behaviour of new sugar based gemini amphiphiles
M. C. A. Stuart, M. Scarzello, J. E. Klijn, E. J. Boekema, J. B. F. N Engberts

26. Peptide-nanotube formation by lysine based lipoamino acids
L. Ziserman, A. Mor, D. Danino

27. Advances in biological microanalysis using event streamed spectrum imaging and programmed beam acquisition
Peter Ingram, Scott Davilla, Ann LeFurgey

28. 3D-correlation-averaging for membrane-protein-crystals
P. J. B. Koeck, P. Purhonen, R. Alvang, B. Grundberg, H. Hebert

29. 8 Å cryo-EM structure of the giant hemoglobin from the planorbid snail Biomphalaria glabrata

J. Markl, F. Depoix, A. Moeller, W. Gebauer

30. Structure of Pex5p and Pex5-20 complexes in the yeast Hansenula polymorpha. Pex20p causes a conformational change upon binding to Pex5p tetramers involved in peroxisomal protein transport
Kasia Moscicka, Sandra H. Klompmaker, Dongyuan Wang, Ida J. Klei, Egbert J. Boekema

31. Structure of OprM-MexA interacting complex revealed by cryo electron tomography
S. Trépout, J C. Taveau, H. Benabdelhak, A. Ducruix, A. S. Frangakis, O. Lambert

L2. Cell Structure and Dynamics

32. Pleiomorphic viruses revealed by cryo tomography: the structure of coronaviruses
M. Bárcena, W. Baterlink, G. T. Oostergetel, A. Verklijk, P. J. M. Rottier, A. J. Koster, B. J. Bosch

33. Combined structural and chemical analysis of unique anammox bacteria that contain a prokaryotic organelle
Laura Niftrik, Willie J. C. Geerts, Elly G. Donselaar, Bruno M. Humbel, Arie J. Verkleij, Marc Strous, Mike S. M. Jetten

34. Structural and functional considerations on the 3-D organization of the fenestrated cytoplasm of hepatic endothelial cells
F. Braet, W. Geerts, J. Riches, S. Satchell, E. Wisse, P. Frederik

35. Molecular architecture of the presynaptic compartment studied by cryo-electron tomography
R. Fernández-Busnadiego, B. Zuber, U. Maurer, G. W. K. Kong, M. Cyrklaff, J. Dubochet, W. Baumeister, V. Lu?i?

36. Synapses in high pressure frozen Caenorhabditis elegans

J. Hegermann, S. Eimer

37. High resolution EM-tomography of a whole vitrified cell
H. Heijnen, H. Nispen tot Pannerden, F. Haas

38. Evaluation of beam damage in catalase crystals observed in vitrified sections
Hong-Mei Han, Bryant Gibson, Jacques Dubochet

39. Urothelial fusiform vesicles are formed in post-Golgi compartment
S. Hudoklin, R. Romih, J. Neumüller, M. Pavelka, K. Jezernik

40. Localisation of GFP and GFP tagged PKD2 in cultivated pancreatic cancer cells using high-pressure freezing
H. Krisp, G. Wichert, T. Seufferlein, P. Walther

41. Cytomegalovirus membrane envelopment revisited — a STEM-tomography study based on high-pressure freezing and freeze substitution
S. Landwehr, J. Einem, T. Mertens, P. Walther

42. Electron tomography & template matching of biological membranes
M. N. Lebbink, W. J. Geerts, E. Donselaar, B. M. Humbel, J. A. Post, L. O. Hertzberger, A. J. Koster, A. J. Verkleij

43. Correlated Microscopy: From Dynamics to Structure
S. Lepper, M. Kudryashev, A. Sartori, S. Bohn, M. Cyrklaff, F. Frischknecht

44. 3DEM-analyses of Golgi apparatus subunits during physiologic and pathologic reorganizations
J. Neumüller, M. Vetterlein, C. Meißlitzer-Ruppitsch, C. Weiss, A. Ellinger, M. Pavelka

45. Visualization of the 80s ribosome in situ using cryo electron tomography of vitreous sections
J. Pierson, J. J. Fernández, E. Bos, H. Gnäegi, J. L. Carrascosa, M. Vos, P. J. Peters

46. Three-dimensional analysis of the intermediate filament network using SEM-tomography
M. Sailer, S. Lück, V. Schmidt, M. Beil, G. Adler, P. Walther

47. The apical vesicles of the tuft cells in the main excretory duct of the rat submandibular gland by EFTEM-TEM tomography
A. Sato, J. Kodama, Y. Inoue, Y. Sawa, T. Oikawa

48. (S)TEM Dual Axis Tomography
Andreas Voigt, Alevtyna Yakushevska, Felix Haas, Wim Voorhout, Remco Schoenmakers

49. STEM tomography of high-pressure frozen cell monolayers
P. Walther, S. Landwehr, H. Krisp, T. Seufferlein, T. Mertens, G. Adler

50. Multimodality of pericellular proteolysis in cancer cell invasion
Peter Friedl

51. Photonic crystal type nanoarchitectures in butterfly wing scales
Z. Vértesy, K. Kertész, Zs. Bálint, G. Márk, L. Tapasztó, J. P. Vigneron, L. P. Biró

52. Iridovirus-like viruses of Lacerta monticola from Serra da Estrela, Portugal
A. P. Alves de Matos, M. F. Caeiro, Guilherme Costa, Teresa Granja, Tibor Papp, Rachel Marschang

53. Structural and ultrastructural investigations on the in vitro effects of plant extracts in chicken and rabbit whole blood cultures
L. Barbu-Tudoran, M. Spanu, C. Craciun, C. V. Mihali

54. Fine structural characterization of cloned human endogenous retrovirus HERV-K113
Klaus Boller, Kurt Schönfeld, Ralf R. Tönjes

55. The procaine hydrochlorate effect onto the corpuscular anthocyans from the vacuolar sap of different plant cells
D. Cachita, A. Ardelean, C. Craciun, V. Turcus, L. Barbu-Tudoran

56. Comparative anatomical and ultrastructural investigations on normal and vitroplantlets leaves from Pistia stratiotes L.
Dorina Cachi??-Cosma, Daniela Beles, C. Craciun, L. Barbu-Tudoran

57. Autophagy of mitochondria in brown adipocytes of chemically thyroidectomised rats
M. ?aki?-Miloševi?, A. Kora?, M. Ukropina

58. Ultrastructural studies of immune organs in antigen primed chickens treated with vegetal extractions
C. Craciun, M. Spanu, A. Ardelean

59. Structural features of HIV-1 immunologic activation
A. Foglia

60. Functions of actin-binding proteins in the cell nucleus
J. Hofmannová, M. Maninová, V. Filimonenko, P. Hozák

61. Ultrastructural mechanisms of entamoebae movement system
Hovnanyan Margarita, Hovnanyan Karlen, Asatryan Rosa

62. White adipocytes transdifferentiation into brown adipocytes induced by triiodothyronine
A. Korac, M. Cakic-Milosevic, M. Ukropina, M. Grubic, K. Micunovic, V. Petrovic, B. Buzadzic, A. Jankovic, A. Vasilijevic, B. Korac

63. Ultrastructural studies of the tegument of cestodes (Platyhelminthes): phylogenetic implications
C. Levron

64. Crystallization stages of the CaCO3 deposits in the earthworm’s calciferous gland
J. Méndez, J. B. Rodríguez-González, R. Alvarez-Otero, M. J. I. Briones, L. Gago-Duport

65. Scanning electron microscopic analysis of heavy metal resistant microorganisms
S. Nietzsche, A. Schmidt, E. Kothe, M. Westermann, W. Richter

66. Characterization of mouse embryoid bodies by Scanning Electron Microscopy (SEM) and Confocal Laser Scanning Microscopy (CLSM)
C. Nogués, R. Arroyo, N. Gaztelumendi

67. Stage-dependent localization of mitochondrial DNA during the cell cycle of Euglena gracilis Z by immunogold electron microscopy
T. Osafune, N. Kiyohara, I. Watanabe, T. Ehara

68. The virus-inducted structures in RNA virus infected macrophages
N. G. Plekhova, L. M. Somova, E. V. Pustovalov

69. Surface layers of ore-leaching Bacteria and Archaea
R. Rachel, A. Klingl, C. Neuner, W. Depmeier, G. Schmalz, M. Thomm, H. Huber

70. Structure of Bacillus anthracis spores analyzed by CEMOVIS
M. Sachse, G. Pehau-Arnaudet, E. Couture-Tosi, J. -L. Ranck, P. Sylvestre, M. Mock

71. Insights into the role of pUL71 during HCMV morphogenesis
M. Schauflinger, S. Landwehr, P. Walther, Th. Mertens, J. Einem

72. Fine structure of the genital chamber of female Chrysomya megacephala (F.) (Diptera: Calliphoridae)
Sirisuda Siriwattanarungsee, Tarinee Chaiwong, Kom Sukontason, Urai Chaisri, Kabkaew L. Sukontason

73. Ultrastructure of the testes and spermatozoa of the blow fly Chrysomya megacephala (F.) (Diptera:Calliphoridae)
Tarinee Chaiwong, Kom Sukontason, Urai Chaisri, Hiromu Kurahashi, Jimmy K. Olson, Udom Chaithong, Kabkaew L. Sukontason

74. Biosynthesis of cellulose in red algae
I. Tsekos

75. Light and electron microscopy aspects of the glandular sessile hairs from the vitroplantlet leave of Drosera rotundifolia

V. Turcu?, D. Cachi??, C. Cr?ciun, A. Ardelean, L. Barbu-Tudoran, C. Mihali

76. Ultrastructure of selected Euglena species in relation to their taxonomy
M. Weidinger, E. Kusel-Fetzmann

77. Confocal Microscopy Reveals Molecular Interactions Between DNA-binding Drugs and Chromatin in Live Cells
J. W. Dobrucki, K. Wojcik

78. Fluorescence microscopy methods for measuring the mobility and stability of molecules in 3-D samples
K. Braeckmans, D. Vercauteren, H. Deschout, D. Mazza, A. Diaspro, S. C. Smedt, J. Demeester

79. Phasor-FLIM analysis of FRET for homotypic and heterotypic non-covalent interactions: stimulated membrane receptors in live cells
V. R. Caiolfa, E. Gratton, M. Zamai

80.
In vitro immunofluorescence and ultrastructural analysis of the expression of noncollagenous matrix proteins in human pulpal cells induced by mineralizing factors
V. E. Arana-Chavez, E. F. Martinez, T. A. G. Donato

81. Differentiation-dependent Golgi fragmentation in the bladder urothelial cells in vitro
Kristijan Jezernik, Mateja Erdani Kreft, Alexander A. Mironov

82. Monitoring Mitochordria Dynamics in Living Cell System by Confocal Microscopy
C. -Y. Lin, L. -T. Li

83. The freeze-fracture replica immunolabeling technique for the study of lipid droplet biogenesis and function
H. Robenek, I. Buers, O. Hofnagel, M. J. Robenek, D. Troyer, N. J. Severs

84. Dynamics of epigenome duplication, accessibility and translation
M. C. Cardoso

85. Controlled light exposure microscopy reveals telomeric microterritories throughout the cell cycle
W. H. Vos, R. A. Hoebe, G. Joss, E. Manders, P. Oostveldt

86. Calibration in fluorescence correlation spectroscopy for measurements of stem cell differentiation kinetic
D. Dumas, B. Riquelme, H. Castellini, L. Basciano, N. Isla, J F Stoltz

87. Spectral methods for cells and particles identifications
N. Koltovoy

88. 3D realistic visualization of supramolecular assemblies
D. Larivière, E. Fourmentin

89. Heterosis in Arabidopsis thaliana: Structural aspects in mature and germinating seeds from hybrid and parental lines
E. Maximova, A. Blacha, Th. Altmann, M. Udvardi

90. Colour visualization of red blood cells in native smears by the new method reflected light microscopy
A. Paiziev, V. Krakhmalev

91.
In vitro culture of Trigonella foenum-graecum plantules and their anatomic characterization
C. Pribac, A. Ardelean

92. Live cell imaging by SEM-hosted X-ray microscope in “water window” energy range
A. Sasov

93. Microscopic anatomy and mineral composition of cuticle in amphibious isopods Ligia italica and Titanethes albus (Crustacea:Isopoda)
J. Štrus, N. Žnidarši?, S. Hild, A. Ziegler

94. In vivo imaging and quantification of the continuous keratin filament network turnover
R. Windoffer, A. Kölsch, S. Wöll, T. Würflinger, T. Aach, R. E. Leube

L3. Microscopy advances in the life sciences

95. Necrotic cell death, a controlled way of cellular explosion
Tom Berghe, Nele Vanlangenakker, Eef Parthoens, Wies Deckers, Michael Devos, Nele Festjens, Peter Vandenabeele

96. Dissecting mitochondrial protein distributions using sub-diffraction resolution fluorescence microscopy
S. Jakobs

97. High Thoughput, High Content Tissue Cyotometry
Peter T. C. So

98. Focus on the vascular wall: Imaging of large arteries using two-photon microscopy
M. A. M. J. Zandvoort, R. T. A. Megens, D. W. Slaaf, M. G. A. oudeEgbrink

99. High resolution confocal Ca2+ imaging of the pulmonary neuroepithelial body microenvironment in lung slices
I. Brouns, I. Proost, I. Pintelon, R. Lembrechts, J. -P. Timmermans, D. Adriaensen

100. Two photon excitation microscopy and SHG imaging as a tool for visualisation of type I and type II collagen, and their use in tissue engineering
Z. Burdíková, E. Filová, M. Rampichová, P. Bianchini, M. ?apek, E. Amler, L. Kubínová

101. Testing calibration standards for confocal and two-photon microscopy
O. Chernyavskiy, L. Kubínová

102. Distribution of the voltage-gated delayed-rectifier K+ subunits, Kv1.1 and Kv1.2, in the adult murine enteric nervous system
A. Costagliola, L. Nassauw, J. -P. Timmermans

103. Observation of Ventricular Myocyte Morphology in Long Term Culture using High Resolution Confocal Imaging
K. Hammer, S. Ruppenthal, Martin Oberhofer, A. Vecerdea, L. Kaestner, P. Lipp

104. A magnetic field enhances the inmunofluorescence signal in a lymphocytic cell
N. V. Joshi, Reyes, L. Barboza, L. Berrueta

105. Novel 515 nm DPSS-laser brings excitement in life cell imaging
L. Kaestner, P. Lipp, D. Gegerfelt, H. Karlsson, E. Illy, J. Hellström

106. Confocal laser scanning microscopy study on human fissure caries stained with alizarin red
S. M. Masudi, N. I. M. Salleh

107. Non-invasive skin tissue characterization using non-linear spectral imaging microscopy
J. A. Palero, H. S. Bruijn, A. Ploeg van den Heuvel, H. J. C. M Sterenborg, H. C. Gerritsen

108. Force Spectroscopy reveals mechanical structure of capsids
G. J. L. Wuite, W. H. Roos, C. Uetrecht, N. Watts, P. Wingfield, A. C. Steven, A. J. R. Heck, J. L. Carrascosa, C. F. Schmidt

109. Manipulating and imaging molecular motors with optical traps, single-molecule fluorescence and atomic force microscopy
C. F. Schmidt, L. C. Kapitein, B. H. Kwok, J. S. Weinger, T. M. Kapoor, E. J. G. Peterman, I. A. T. Schaap, C. Carrasco, Pedro J. Pablo, Stefan Lakämper

110. Mechanical stability of ethanol-induced A-DNA
J. R. Arias-Gonzalez, S. Hormeño, B. Ibarra, J. M. Valpuesta, J. L. Carrascosa

111. Manipulation of the mechanical properties of a virus by protein engineering
P. J. Pablo, C. Carasco, M. Castellanos, M. G. Mateu

112. A single-molecule mechanical assay to study DNA replication coupled to DNA unwinding
B. Ibarra, J. A. Morin, R. Arias, S. Hormeño, M. Salas, J. M. Valpuesta, J. L. Carrascosa

113. Generation of multiple DNA lesions at subnuclear resolution by multi-photon irradiation
D. Träutlein, M. Deibler, E. Ferrando-May, A. Leitenstorfer

114. Light and electron microscopy of phagosomes
Gareth Griffiths, David Liebl, Christopher Bleck, Andreas Merz, Paul Walther, Jacques Dubochet, Benoit Zuber

115. The Use of Electron Microscopy in the Diagnosis of Human Microsporidial Infection — the Manchester, UK, Experience
Alan Curry

116. Overview of the impact of biological microanalysis in health and disease
Ann LeFurgey, Peter Ingram

117. Immunolocalization of FOXP3 in HCV-infected liver biopsies. Preliminary observations.
F. D’Amico, A. Amoroso, M. Consolo, E. Skarmoutsou, B. Mauceri, G. Malaponte, F. Stivala, C. M. Bruno, S. Neri, M. C. Mazzarino

118. Ultrastructural and Intracellular Elemental Composition Analysis of Human Hematopoietic Cells During Cold Storage in Preservation Solutions
F. Arrebola, F. J. Cañizares, M. A. Cubero, M. M. Serrano, M. A. Robles, E. Fernández-Segura

119. Reconstitution and morphological characterisation of an original Human Endogenous Retrovirus
N. Bannert, N. Beimforde, L. Möller, G. Holland, M. Laue, R. Kurth

120. Cell death in human articular chondrocyte: an ultrastructural study in micromass model.
M. Battistelli, A. D’Emilio, E. Olivotto, S. Pagani, R. Borzì, A. Facchini, E. Falcieri

121. Pathomorphological diagnostic of paraffin embedded versus epon embedded cardiac tissues with transmission electron microscope analysis
J. Bornemann, B. Hermanns-Sachweh, N. Gaßler

122. Interaction of carbon nanotubes with macrophages: STEM and EELS approach
Cyrill Bussy, Nathalie Brun, Julien Cambedouzou, Sophie Lanone, Alain Grodet, Vasile Heresanu, Jorge Boczkowski, Pascale Launois, Claudie Mory

123. The ultastructural effect of nitrogen mustard gas on rat brain and therapeutic value of proanthocyanidin
B Can, A Tekiner, A Köse Sarg?n, O Yücel, M. A Bayar

124. Assembly and maturation of pestiviruses
S. Deike, M. König, H. -J. Thiel

125. Analysis of radiation-induced bystander effects using high content screening
B. Dieriks, W. Vos, P. Oostveldt

126. Electron microscopic investigations on normal and dexamethasone applied rat placentas
H. Er, E. T. Korgun, Ç. Çelik-Özenci, M. Saçan, M. Asar

127. Changes in dental enamel birefringence after CO2 laser irradiation through fluoride gel -a pilot study
M. Esteves-Oliveira, C. P. Eduardo, F. Lampert, C. Apel

128. SEM and light microscopic examination and morphometric analysis of temporal changes in intimal and medial connective tissue component of human carotid arteries
O. Evirgen, T. Zeyrek, N. Apaydin, H. Ilkay, O. Semiz

129. Ultrastructure of human amniotic membrane covering villous chorion and smooth chorion in preeclampsia
O. Evirgen, H. ?lkay, S. Akku?, O. Semiz, A. Heper Okçu, R. Aytaç

130. Changes in Intracellular Sodium, Chlorine, and Potassium Content in Hematopoietic Cells after Hypotermic Storage
E. Fernández-Segura, F. Arrebola, M. A. Cubero, F. J. Cañizares, M. A. Robles, P. Navarrete

131. Requirements for a bright future of electron microscopy in the rapid laboratory diagnosis of infectious diseases
H. R. Gelderblom

132. Changed thickness of zona pellucida as a result of stimulated ovulation
B. Jod?owska-J?drych, M. J?drych, W. Matysiak, K. Czerny

133. Mozaic microscopy of pancreatic islets
D. Kalicharan, K. A. Sjollema, F. Dijk, J. J. L. Want, B. N. G. Giepmans

134. Microscope history Database
N. Koltovoy

135. Interaction of H-Ras Transformed Cell Line with Folic Acid Modified Magnetic Nanoparticles and Detection by Transmission Electron Microscope
M. Kutlu, N. Saltan, R. Say, A. ??can

136. Sedimentation of suspensions for diagnostic thin section electron microscopy
M. Laue, N. Bannert

137. Evaluation of rofecoxib administration influence on ultrastructural image of the kidney
W. Matysiak, B. Jod?owska-J?drych, E. Kifer-Wysocka, J. Romanowska-Sarlej, K. Czerny

138. Microstructural changes produced by Pulsed Electric Fields in liquid whole egg studied by Transmission Electron Microscopy (TEM)
I. Pérez-Munuera, R. Marco-Molés, I. Hernando, A. Quiles, A. Puig

139. Microstructural changes in dough treated by Glucose oxidase (GOX) and Transglutaminase (TG), studied by Scanning Electron Microscopy (SEM)
I. Pérez-Munuera, A. Quiles, A. Puig, I. Hernando

140. Cytoplasmic K/Na balance in the cardiomyocyte of young and old rats
M. A. Pogorelova, A. G. Pogorelov

141. Hyperspectral imaging — a novel concept for marker free chromosome characterization
K. Rebner, T. Merz, R. W. Kessler

142. Poxviruses: Morphogenesis of an Orthopoxvirus (CPXV) and an Avipoxvirus (FPXV) in host tissue
S. Richter, P. Wernsdorf

143. Ultrastructural alterations of methotrexate in mouse kidney
Ay?e Köse Sarg?n, Emin O?uzhan O?uz, Belgin Can, Yüksel Saran

144. Ultrastructural gadolinium evidence in skin of a patient with Nephrogenic Fibrosing Dermopathy
J. A. Schroeder, C. Weingart, T. Vogt, I. Hausser, F. Hofstaedter, B. K. Krämer

145. Nephrotoxicity induced by inorganic Hg(II) and Pb(II): a microscopic and biochemical in vitro study
A. Stacchiotti, F. Morandini, F. Bettoni, L. F. Rodella, P. Grigolato, A. Lavazza, M. F. Aleo

146. Tissue distribution of peroxisomes in zebrafish
M. Stevens, O. Krysko, M. Baes, M. Espeel

147. An analysis of the ultrastructure of matured HIV
I. Takahashi, M. Takama, A. M. Ladhoff

148. Trichinella spiralis and Trichinella britovi cuticle and hypodermal glands Ultrastructure
M. Tosti

149. Ultrastructural analysis of lysosomal storage diseases: effects of therapy
Marius A Bergh Weerman, Josanne Cox-Brinkman, Anouk C Vedder, Frits A. Wijburg, Carla E. M. Hollak, Sandrine Florquin

L4. Sample preparation and identification of molecular targets

150. Immunolabeling for Electronmicroscopy
G. Posthuma

151. Molecular Organisation of Cadherins in Intercellular Adhesion Junctions by Cryo-Electron Tomography of Vitreous Sections
Ashraf Al-Amoudi, Achilleas Frangakis

152. Ultrastructural observations of indium in the lactating mammary gland cells
Ayadi Ahlem, Maghraoui Samira, Hili Ali, Tekaya Leila

153. An active mechanism flanks and modulates the export of the small ribosomal subunits
B. Cisterna, M. Malatesta, J. Dieker, E. Prosperi, S. Muller, M. Biggiogera

154. New methods for micro-domain detection in bacterial sacculi
M. A. Pedro, H. Schwarz

155. Subcellular localization of Myogenic Regulatory Factors along skeletal muscle development
E. Falcieri, S. Burattini, L. Biagiotti, P. Ferri, A. D’Emilio, M. Guescini, E. Barbieri, V. Stocchi

156. Immunocytochemical Strategies: LR Resins or Lowicryl, Gold or Peroxidase — Which is Better?
J. A. Hobot, C. J. Ruhland

157. Elemental Analysis in Electron Microscopy for Medical Diagnostics
L. Jonas, S. Schäd

158. Synaptic localization of KCa1.1 potassium channels in central neurons revealed by postembedding immunogold and SDS-digested freeze-fracture replica labelling
W. A. Kaufmann, R. Shigemoto, H. -G. Knaus, F. Ferraguti

159. Ultrastructural studies of rod photoreceptor nuclei from SCA7 mouse
C. Kizilyaprak, D. Spehner, D. Devys, L. Tora, P. Schultz

160. Application of electron energy loss spectroscopy for heavy metal localization in unicellular algae
U. Lütz-Meindl, M. Milla, B. Stöger

161. Localization of “flagella” proteins in the Archaeon Ignicoccus hospitalis

C. Meyer, D. Müller, S. Gürster, H. Huber, R. Wirth, R. Rachel

162. Detection of gold nanoparticles by Autrata YAG detector in FE SEM working in cryo mode
J. Nebesarova, M. Vancova, S. Hucek, J. Vanecek

163. Correlative cryo-fluorescence and electron microscopy
A. Rigort, A. Leis, M. Gruska, A. Sartori, W. Baumeister, J. M. Plitzko

164. The effect of hypometabolizing molecules on transcription as shown by a new two-step pulse-chase method
A. Spedito, M. Malatesta, M. Biggiogera

165. Cryofixation and Freeze-Substitution Combined with Tokuyasu Cryo-section Labelling
Y. -D. Stierhof, H. Schwarz

166. The Drosophila active zone architecture: combining confocal, STED and transmission electron microscopy
C. Wichmann, W. Fouquet, S. Mertel, D. Owald, M. Dyba, S. Eimer, Stephan J. Sigrist

167. Distribution and function of amorphous CaCO3 and calcite within the tergite cuticle of terrestrial isopods (Crustacea)
A. Ziegler, S. Hild

168. A symbiosis: tracking cell signaling with expression probes, quantum dots and a programmable array microscope (PAM)
M. G. Botelho, W. Caarls, S. Kantelhardt, A. Cambi, G. Hagen, D. J. Arndt-Jovin, T. M. Jovin

169. Golgi twins in mitosis revealed by genetically encoded tags for live cell imaging and correlated electron microscopy
Ben N. G. Giepmans, Guido M. Gaietta, Thomas J. Deerinck, Stephen R. Adams, Roger Y. Tsien, Mark H. Ellisman

170. Understanding the 3D architecture of organelle bound protein complexes using cryo-electron tomography of frozen hydrated sections and immunogold; our next great challenge
Peter J. Peters, Jason Pierson, Diane Houben, Musa Sani, Sue Godsave, Pekka Kujala, Matthijn Vos, Erik Bos, Hans Jansen, Maaike Zon, Nicole Wel

171. Cryo-electron microscopy of vitreous sections
B. Zuber, M. Chami, C. Houssin, J. Dubochet, G. Griffiths, Mamadou Daffé

172. The visualization of viruses in the low-voltage electron microscope
H. Bielniková, J. Nebesá?ová, M. Vancová

173. Exploring HPF/FS methods for sensitive immunogold labeling on resin sections
P. Hozák, A. A. Philimonenko, J. Janá?ek, P. Janda

174. Low contrast of the ER-membranes in the high pressure frozen, freeze substituted specimens
M. Lindman, H. Vihinen, E. Jokitalo

175. HPF of cultured cell monolayers: towards a standard method for high pressure freezing, freeze substitution and electron tomography acquisition
C. López-Iglesias, J. Fontana, S. Ruíz, C. Risco

176. Life-like physical fixation of large samples for correlative microscopy
M. S. Lucas, M. Wölfel, F. Lucas, R. Wepf

177. Integrating automated single, double and triple in situ molecular detection with imaging for the analysis of inter- and intracellular events in the seminiferous tubule
K. Manova, Y. Romin, A. Barlas, S. Gonzalez, M. Turkekul, T. Tong, S. Suh, V. Gueorguiev, Z. Lazar

178. Freeze-substitution in Epon: An attempt to combine immunolabeling and improvement of structural preservation
E. M. Schraner, S. Leisinger, M. Müller, P. Wild

179. The Shape of Caveolae is Omega-like after Chemical Fixation and Cup-like after Cryofixation
M. Westermann, F. Steiniger, S. Nietzsche, G. Hause, C. Lemke, W. Richter

L5. Hybrid methods and approaches in microscopy

180. Integrated laser and transmission electron microscopy
A. V. Agronskaia, J. A. Valentijn, L. F. Driel, B. M. Humbel, A. J. Verkleij, A. J. Koster, H. C. Gerritsen

181. Correlative 3D microscopy: CLSM and FIB/SEM tomography used to study cellular entry of vaccinia virus
M. S. Lucas, P. Gasser, M. Günthert, J. Mercer, A. Helenius, R. Wepf

182. Strategies for the morphological analysis of hydrated and life-like preserved biomedical material in the SEM
H. Hohenberg, R. Reimer, M. Warmer, B. Holstermann

183. Denaturation of metaphase chromatin plates observed by transmission electron microscopy and atomic force microscopy in aqueous solution
I. Gállego, X. Sisquella, X. Fernàndez-Busquets, J.R. Daban

184. Discovery of a Nuclear Structure in UVC-induced Apoptotic Cells by Integrated Laser Electron Microscopy
B. M. Humbel, M. A. Karreman, A. V. Agronskaia, A. J. Verkleij, H. C. Gerritsen, A. F. M. Cremers

185. Analysis of biomineral formation in three-dimensional micro-mass stem cell cultures
L. Lammers, U. Meyer, J. Handschel, C. Naujoks, H. P. Wiesmann

186. Comparative structural studies of highly-filled polymethacrylates with light microscopy, SEM, TEM and AFM
C. Lurtz, L. Schmitt, D. Behrend, K. Sternberg, K. -P. Schmitz

187. Cell surface characteristics as reporters for cellular energy state
H. Pluk, H. Croes, B. Lich, B. Wieringa, J. Fransen

188. Analysis of multimodal 3D microscopy measurements
M. Ritter, Axel Kranzmann, M. Hemmleb

189. Permanent plastid — nuclear complexes (PNCs) in plant cells
T. Selga, M. Selga

190. Cell imaging by dynamic secondary ion mass spectrometry (SIMS): basic principles and biological applications
Alain Croisy, Jean-Luc Guerquin-Kern, Ting-Di Wu

191. Water imaging in cells cryosections by electron energy loss spectroscopy
J. Michel, C. Terryn, G. Balossier

192. Study of the cellular uptake of Pt nanoparticles in human colon carcinoma cells
H. Blank, R. Schneider, P. Brenner, D. Gerthsen, M. Esselen, D. Marko, M. Crone, M. Türk, T. Muller, S. Bräse

193. Ion spectrometry and electron microscopy correlative imaging for accurate comprehensive trace element localization at subcellular level, in normal and pathological keratinocytes
J. Degrouard, E. Larras-Regard, D. Jaillard

194. Calcium Spark Detection and Analysis in Time Series of Two-Dimensional Confocal Images
K. Hammer, A. Flockerzi, A. Zeug, P. Lipp, L. Kaestner

195. Visualisation of the attachment, possible uptake and distribution of technical nanoparticles in cells with electron microscopy methods
Armin Springer, Wolfgang Pompe, Susanne Bastian, Maria Iwe, Hrissanthi Ikonomidou, Wibke Busch, Dana Kühnel, Annegret Pothoff, Volkmar Richter, Michael Gelinsky

Nyckelord: Physics, Physics, general