Precision Medicine, CRISPR, and Genome Engineering

Part I. Introduction to the CRISPR Revolution

1. Viral Vectors, Engineered Cells and the CRISPR Revolution
James E. DiCarlo, Anurag Deeconda, Stephen H. Tsang

2. Combining Engineered Nucleases with Adeno-associated Viral Vectors for Therapeutic Gene Editing
Benjamin E. Epstein, David V. Schaffer

Part II. CRISPR in Model Systems

3. From Reductionism to Holism: Toward a More Complete View of Development Through Genome Engineering
Rebecca K. Delker, Richard S. Mann

4. A Transgenic Core Facility’s Experience in Genome Editing Revolution
Celvie L. Yuan, Yueh-Chiang Hu

5. Genome Editing to Study Ca2+ Homeostasis in Zebrafish Cone Photoreceptors
Susan E. Brockerhoff

6. CRISPR: From Prokaryotic Immune Systems to Plant Genome Editing Tools
Anindya Bandyopadhyay, Shamik Mazumdar, Xiaojia Yin, William Paul Quick

Part III. The Future of CRISPR

7. Target Discovery for Precision Medicine Using High-Throughput Genome Engineering
Xinyi Guo, Poonam Chitale, Neville E. Sanjana

8. CRISPR in the Retina: Evaluation of Future Potential
Galaxy Y. Cho, Sally Justus, Jesse D. Sengillo, Stephen H. Tsang

9. The Future of CRISPR Applications in the Lab, the Clinic and Society
Soren H. Hough, Ayokunmi Ajetunmobi

Nyckelord: Life Sciences, Regenerative Medicine/Tissue Engineering, Stem Cells, Ophthalmology, Human Genetics, Agriculture