Advanced Technologies for Protein Complex Production and Characterization

Part I. Introduction

1. Protein Complex Production from the Drug Discovery Standpoint
Ismail Moarefi

2. Choose a Suitable Expression Host: A Survey of Available Protein Production Platforms
Francisco J. Fernández, M. Cristina Vega

Part II. Prokaryotic Expression Hosts

3. ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts
Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald, Ian Collinson, Christiane Schaffitzel, Imre Berger

4. Complex Reconstitution and Characterization by Combining Co-expression Techniques in Escherichia coli with High-Throughput
Renaud Vincentelli, Christophe Romier

5. Membrane Protein Production in E. coli for Applications in Drug Discovery
Harm Jan (Arjan) Snijder, Jonna Hakulinen

6. Cell-Free Synthesis of Macromolecular Complexes
Mathieu Botte, Aurélien Deniaud, Christiane Schaffitzel

7. A Bacillus megaterium System for the Production of Recombinant Proteins and Protein Complexes
Rebekka Biedendieck

8. Protein Complex Production in Alternative Prokaryotic Hosts
Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, M. Cristina Vega

Part III. Lower Eukaryotic Expression Hosts

9. Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts
Francisco J. Fernández, Miguel López-Estepa, Javier Querol-García, M. Cristina Vega

10.
Leishmania tarentolae for the Production of Multi-subunit Complexes
Tomoaki Niimi

11. Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes
Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, Teresa Suárez, M. Cristina Vega

Part IV. Higher Eukaryotic Expression Hosts

12. Fundamentals of Baculovirus Expression and Applications
Thomas A. Kost, Christopher W. Kemp

13. The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics
Duygu Sari, Kapil Gupta, Deepak Balaji Thimiri Govinda Raj, Alice Aubert, Petra Drncová, Frederic Garzoni, Daniel Fitzgerald, Imre Berger

14. Fundamentals of Expression in Mammalian Cells
Michael R. Dyson

15. Assembling Multi-subunit Complexes Using Mammalian Expression
Bahar Baser, Joop Heuvel

Part V. Plant Expression

16. Microalgae as Solar-Powered Protein Factories
Franziska Hempel, Uwe G. Maier

17. Strategies and Methodologies for the Co-expression of Multiple Proteins in Plants
Albert Ferrer, Monserrat Arró, David Manzano, Teresa Altabella

18. Transient Expression Systems in Plants: Potentialities and Constraints
Tomas Canto

Part VI. Complex Reconstitution

19. Complex Reconstitution from Individual Protein Modules
Jérôme Basquin, Michael Taschner, Esben Lorentzen

20. Structural Reconstruction of Protein-Protein Complexes Involved in Intracellular Signaling
Klára Kirsch, Péter Sok, Attila Reményi

Part VII. Biophysical Methods to Assess Protein Complexes

21. The Use of Small-Angle Scattering for the Characterization of Multi Subunit Complexes
Adam Round

22. Application of Nuclear Magnetic Resonance and Hybrid Methods to Structure Determination of Complex Systems
Filippo Prischi, Annalisa Pastore

Nyckelord: Life Sciences, Protein Science, Protein Structure, Systems Biology