Logga in

RNA Interference (RNAi)

 - RNA Interference (RNAi), e-bok

32,35€

E-bok, ePUB, Adobe DRM-skydd
ISBN: 9781118829448
DRM-begränsningar

Skriva ut30 sidor with an additional page accrued varje dag, capped at 30 sidor
Kopiera till urklipp5 utdrag

The 2006 Nobel Prize in Physiology or Medicine was awarded to Andrew Z. Fire and Craig C. Mellofor their discovery of RNA interference (RNAi). RNAi is a biological process in which RNAmolecules can silence (inhibit) or up- or down-regulate gene expression, typically by causing the destruction of specific messenger RNA (mRNA)molecules. This collection of articles begins with an introduction to RNAi, and proceeds to describe uses of this technology in various approaches to disease treatment, including gene therapy. Several laboratory protocols for silencing genes via RNAi are also provided, as are protocols for down-regulating and then rescuing those down-regulated genes, which demonstrates specificity of the approach.

This e-book — a curated collection from eLS, WIREs, and Current Protocols — offers a fantastic introduction to the field of RNA interference for students or interdisciplinary collaborators.

Table of Contents:

Introduction

Overview of Gene Silencing by RNA Interference
Current Protocols in Nucleic Acid Chemistry
Daniel H. Kim, John J. Rossi

Biogenesis and function of endogenous and exogenous siRNAs
WIREs RNA
Nicholas M. Snead, John J. Rossi

Techniques & Applications

Small Silencing RNAs and Gene Therapy
eLS
Dirk Grimm

RNAi Screening: New Approaches, Understandings, and Organisms
WIREs RNA
Stephanie E. Mohr, Norbert Perrimon

Delivery of RNAi mediators
WIREs RNA
Lance P. Ford, Masoud M. Toloue

Protocols

Silencing of Gene Expression in Cultured Cells Using Small Interfering RNAs
Current Protocols in Cell Biology
Kumi Sakurai, Pritsana Chomchan, John J. Rossi

Gene Down-Regulation with Short Hairpin RNAs and Validation of Specificity by Inducible Rescue in Mammalian Cells
Current Protocols in Cell Biology
Hoi Tang Ma, Randy Y.C. Poon

Nyckelord: protocols; interference; rna; acid; h; chemistrydaniel; gene; nucleic; introduction overview; current; endogenous; biogenesis; rossi; grimm; screening; approaches; techniques; rnai; new; applications; elsdirk; delivery; mediators, Molecular Techniques, Biomolecules (DNA, RNA, Peptides, etc.), Molecular Techniques, Biomolecules (DNA, RNA, Peptides, etc.)

Utgivare
John Wiley and Sons, Inc.
Utgivningsår
2014
Språk
en
Utgåva
1
Serie
Life Science Research Fundamentals
Sidantal
157 sidor
Kategori
Naturvetenskaper
Format
E-bok
eISBN (ePUB)
9781118829448
Tryckt ISBN
9781118829486

Liknande e-böcker