A Laboratory Guide to the Mammalian Embryo

Preface
Acknowledgements
List of Contributors
Abbreviations
1. Follicle development in vitro, Norah Spears, Evelyn E. Telfer and Alison A. Murray
2. Preparation of gametes, in vitro maturation, in vitro fertilization and embryo recovery, Michelle Lane and David Gardner
3. Culture of the mammalian preimplantation embryo, David Gardner and Michelle Lane
4. Assessment of embryo development and viability, Denny Sakkas, Michelle Lane and David Gardner
5. Micromanipulation : ICSI, Gianpiero Palermo, Queenie V. Neri, Takumi Takeuchi, Lucinda L. Veeck, Zev Rosenwaks
6. Micromanipulation : Biopsy, Joyce Harper and Alpesh Doshi
7. Analysis of intracellular ions: pH and calcium, Michelle Lane, Jay Baltz and David Gardner
8. Nutrient uptake and metabolite production and enzyme activity and regulation, David Gardner and Michelle Lane
9. Metabolic Pathway, Don Rieger
10. Confocal imaging of structural molecules in mammalian gametes, Calvin Simerly, Ricardo Moreno, Joao Ramalho-Santos, Laura Hewitson, and Gerald Schatten
11. Fluorescent in situ hybridization, Leeanda Wilton
12. Assessment of ploidy, telomere length and telomerase activity in oocytes and embryos, W. A. King, D. H. Betts, W. Slimane and P.K. Basur
13. Microscale RNA isolation from mammalian embryos, C. Wrenzycki, A.J. Watson, and M. Calder
14. Relative mRNA transcript abundance in early embryos by Reverse Transcription-Polymerase Chain Reaction (RT-PCR), P. A. De Sousa and A. J. Watson
15. Characterization of novel genes during early development by application of differential display RT-PCR, Dave Natale
16. Two-dimensional protein gel database analysis of embryos, oocytes, and oocyte-associated granulosa cells, K. E. Latham, G. Nourjanova, K. Wigglesworth and J. J. Eppig
17. Antisense disruption of gene expression in the preimplantation embryo, Lisa C Barcroft, D Holstead Jones-Taggart and Andrew J Watson
18. Gene subtraction and analysis, M. A. Sirard and C. Robert
19. Analysis of apoptosis in the preimplantation embryo, Daniel Brison Anthony D Metcalfe, Debra J Bloor, Helen R Hunter, Gerard Brady, Susan J Kimber
20. Cytoplasmic Signaling and Cell Cycle Control in the Mouse Egg and Embryo, D. P. Baluch, C. M. Pauken, and D. G. Capco
21. Human Embryonic Stem, Susan E. Lazendorf, Catherine A. Boyd and Diane L. Wright
22. Spermatogonial Transplantion, Makoto Nagano
23. Somatic Cell Nuclear Transfer, Paul De Sousa, William Ritchie, Andras Dinnyes, Tim King, Lesley Paterson, and Ian Wilmut
24. Cryobiology; slow freezing vitrification of embryos, Tetsunori Mukaida and Magosaburo Kasai

Nyckelord: SCIENCE / Life Sciences / Zoology / Mammals SCI070030