Practical Manual of In Vitro Fertilization

1. Journey of Human Gametes In Vitro: 1978–2010
Zeev Shoham

2. Building the Laboratory
Dean E. Morbeck, Marlena Duke

3. Air Quality Management
Johan Guns, Ronny Janssens, Martine Vercammen

4. Organizational Aspects of the Laboratory in a Tertiary Care ART Center
Nancy L. Bossert, Christopher Jonge

5. Quality Control Management
William R. Boone, H. Lee Higdon

6. Daily, Weekly, and Regular Preparations for the IVF Laboratory
Michael A. Britt, Klaus E. Wiemer

7. Risk and Safety in the IVF Clinic
Peter Sjoblom, Julius Hreinsson

8. Essential Instruments and Disposable Supplies for an IVF Laboratory
Leslie Weikert, Christa Fralick, Klaus E. Wiemer

9. Co2 and Low-O2 Incubators
Marius Meintjes

10. IVF Workstations
Nicolas Prados, Alex C. Varghese

11. Culture Media in IVF: Decisions for the Laboratory
Jason E. Swain, Thomas B. Pool

12. Oocyte Denuding
Roberta Maggiulli, Filippo Ubaldi, Laura Rienzi

13. Assessment of Oocyte Quality
Basak Balaban, Turgay Barut, Bulent Urman

14. Polarization Microscopy
Markus Montag, Maria Köster, Hans Ven

15. Cumulus Cell Gene Expression in Assessment of Oocyte Quality
Dagan Wells

16. Short Culture: Day 1/Day 2/Day 3 Embryo Culture
Patrick Quinn

17. Extended Culture in IVF
David K. Gardner, Michelle Lane

18. In Vitro Maturation of Human Oocytes
Baris Ata, Jack Huang, Ri-Cheng Chian

19. In Vivo Embryo Culture Device
Claude Ranoux

20. Microfluidics for Gamete Manipulation and Embryo Culture
Gary D. Smith, Charles Bormann, Shuichi Takayama

21. Sperm Assessment: Traditional Approaches and Their Indicative Value
Margot Flint, Fanuel Lampiao, Ashok Agarwal, Stefan S. Plessis

22. Sperm Assessment: Novel Approaches and Their Indicative Value
De Yi Liu, Harold Bourne, Claire Garrett, Gary N. Clarke, Shlomi Barak, H. W. Gordon Baker

23. Sperm Processing for IVF
Ralf Henkel

24. PESA/TESA/TESE Sperm Processing
Sandro C. Esteves, Sidney Verza

25. Processing Sperm Samples in HIV-Positive Patients
Thamara Viloria, Marcos Meseguer, Antonio Pellicer, José Remohí, Nicolás Garrido

26. Intracytoplasmic Morphologically Selected Sperm Injection
P. Vanderzwalmen, Magnus Bach, Batsuren Baramsai, A. Neyer, Delf Schwerda, Astrid Stecher, Barbara Wirleitner, Martin Zintz, Bernard Lejeune, S. Vanderzwalmen, Nino Guy Cassuto, Mathias Zech, Nicolas H. Zech

27. Sperm Testing and ICSI Selection by Hyaluronic Acid Binding: The Hyaluronic Acid-Coated Glass Slide and Petri Dish in the Andrology and IVF Laboratories
Gabor Huszar

28. Electrophoretic Sperm Separation
Steven Fleming, John Aitken

29. Magnetic-Activated Cell Sorting of Human Spermatozoa
Enver Kerem Dirican

30. Polscope-Based Sperm Selection
Luca Gianaroli, Cristina Magli, Andor Crippa, Giorgio Cavallini, Eleonora Borghi, Anna P. Ferraretti

31. Intrauterine Insemination
Gautam N. Allahbadia, Rubina Merchant

32. Conventional IVF Insemination
Liesl Nel-Themaat, Thomas Elliott, Ching-Chien Chang, Graham Wright, Zsolt Peter Nagy

33. Intracytoplasmic Sperm Injection
Gianpiero D. Palermo, Queenie V. Neri, Devin Monahan, Takumi Takeuchi, Peter N. Schlegel, Zev Rosenwaks

34. Mechanism of Human Oocyte Activation During ICSI and Methodology for Overcoming Low or Failed Fertilization
Dmitri Dozortsev, Mohammad Hossein Nasr-Esfahani

35. Hydraulic Manipulators for ICSI
Hubert Joris

36. Research Instruments Micromanipulators
Steven Fleming, Catherine Pretty

37. Eppendorf Micromanipulator: Setup and Operation of Electronic Micromanipulators
Ehab Abu-Marar, Safa Al-Hasani

38. The Leica Microsystems’ IMSI System
Christiane Wittemer, Bruno Laborde, Frederic Ribay, Stephane Viville

39. Automated Robotic Intracytoplasmic Sperm Injection
Zhe Lu, Xinyu Liu, Xuping Zhang, Clement Leung, Navid Esfandiari, Robert F. Casper, Yu Sun

40. Oocyte Treatment and Preparation for Microinjection
Thomas Ebner

41. Livestock Production via Micromanipulation
Akira Onishi, Anthony C. F. Perry

42. Pronuclear Scoring in Human In Vitro Fertilization
Lynette Scott

43. Cumulative Morphological Assessment of Embryo Quality
Barry Behr, Aparna Hegde

44. Metabolomics: The ViaMetrics-E ™ Procedure for Assessing Embryo Viability
D. Sakkas, L. Botros, M. Henson, K. Judge, P. Roos

45. Oxygen Consumption as an Indicator of Oocyte and Embryo Viability
Ana S. Lopes

46. Gene Expression Changes During Human Early Embryo Development: New Applications for Embryo Selection
Samir Hamamah, Said Assou, Imène Boumela, Hervé Dechaud

47. Amino Acid Turnover as a Biomarker of Embryo Viability
Christine Leary, Danielle G. Smith, Henry J. Leese, Roger G. Sturmey

48. Real-Time Embryo Monitoring Device for Embryo Selection
Gábor Vajta, Thorir Hardarson

49. Assisted Hatching in IVF
Itziar Belil, Anna Veiga

50. Polar Body Biopsy
Markus Montag, Maria Köster, K. Ven, Hans Ven

51. Cleavage-Stage Embryo Biopsy
Alan R. Thornhill

52. Embryo Biopsy for PGD: Current Perspective
Steven J. McArthur, Don Leigh, Maria Traversa, James Marshall, Robert P. S. Jansen

53. Microarrays and CGH for PGD of Chromosome Abnormalities and Gene Defects
Gary Harton, Santiago Munné

54. Sperm Cryopreservation
Fabio Firmbach Pasqualotto, Eleonora Bedin Pasqualotto, Edson Borges, Ashok Agarwal

55. Slow Freezing of Oocytes
Giovanni Coticchio, Lucia Santis

56. Vitrification: Research in Animal Models
Gábor Vajta

57. Oocyte Vitrification
Ana Cobo

58. Slow Freezing of Embryos
Liesl Nel-Themaat, Ching-Chien Chang, Thomas Elliott, Patricia Bernal, Graham Wright, Zsolt Peter Nagy

59. Vitrification of Embryos
Juergen Liebermann, Joe Conaghan, Zsolt Peter Nagy, Michael Tucker

60. Directional Freezing of Reproductive Cells, Tissues, and Organs
Amir Arav

61. Ovarian Tissue Cryopreservation
Jennifer L. Kulp, J. Ryan Martin, Pasquale Patrizio

62. Assessment of Uterine Receptivity
Hakan Cakmak, Hugh S. Taylor

63. Single Embryo Transfer
Thorir Hardarson, Matts Wikland

64. Ultrasound-Guided Embryo Transfer (Abdominal/Vaginal): An Evidence-Based Evaluation
Ahmed M. Abou-Setta

65. Cumulus-Aided Embryo Transfer
Firuza R. Parikh, Nandkishor J. Naik, Dattatray J. Naik, Dhanajaya Kulkarni

66. Data Management in the ART Laboratory: Requirements and Solutions
Timothy Brown, Bruce R. Gilbert, Bruce R. Gilbert

67. Regulation, Licensing, and Accreditation of the ART Laboratory
Doris Baker

68. Legislation in the United Kingdom
Rachel Cutting

69. Regulation, Licensing, and Accreditation of the ART Laboratory in Europe
Julius Hreinsson, Peter Sjoblom

70. Regulation, Licensing, and Accreditation of ART Laboratories in India
B. N. Chakravarty, Rita Modi

71. Troubleshooting in the Clinical Embryology Laboratory: The Art of Problem-Solving in ART
Kathryn J. Go, Jay C. Patel, Rick Dietz

72. The Role of Mitochondria in the Establishment of Developmental Competence in Early Human Development
Jonathan Blerkom

73. Nuclear and Cytoplasmic Transfer: Human Applications and Concerns
Josef Fulka, Helena Fulka

74. Cytoskeletal Architecture of Human Oocytes with Focus on Centrosomes and Their Significant Role in Fertilization
Heide Schatten, Vanesa Y. Rawe, Qing-Yuan Sun

75. Molecular Mining of Follicular Fluid for Reliable Biomarkers of Human Oocyte and Embryo Developmental Competence
Jonathan Blerkom

Avainsanat: Medicine & Public Health, Endocrinology, Urology/Andrology, Developmental Biology, Cell Biology, Obstetrics/Perinatology