Smith, Rodney
Cell Technology for Cell Products
CHAPTER I. : PERSPECTIVES OF THE BIOTECH INDUSTRY
1. The UK bioprocessing sector
Tony Bradshaw
CHAPTER II. : TRANSCRIPTION TO SECRETION
2. A Novel AIR-induced Gene Expression System in HEK.EBNA Cells and its Applications
Nicola Susann Werner, Sabine Geisse, Wilfried Weber, Martin Fussenegger
3. Towards Stronger Gene Expression - a Promoter's Tale
Stephan Kalwy, James Rance, Alison Norman, Robert Gay
4. Improving the Expression of a Soluble Receptor:Fc fusion Protein in CHO Cells by Coexpression with the Receptor Ligand
Gene W. Lee, Jill K. Fecko, Ann Yen, Deb Donaldson, Clive Wood, Scott Tobler, Suresh Vunuum, Yin Luo, Mark Leonard
5. Growth, Apoptosis and Functional Genomics Analysis of CHO-K1 Over-Expressing Telomerase
Francesco Crea, Donatella Sarti, Francesco Falciani, Mohamed Al-Rubeai
6. Microarray Analysis of Metabolically Engineered NS0 Cell Lines Producing Chimeric Antibody
G. Khoo, F. Falciani, M. Al-Rubeai
7. Optimizing Medium for Transient Transfection
Z.-H. Geng, W. Nudson, L. Davis, S. Luo, K. Etchberger
8. Establishment of Recombinant CHO Cell Lines Under Serum-free Conditions
Maya Kuchenbecker, Bernd Rehberger, Michael Schriek, Judith Gackowski, Ralf Fehrenbach
9. Novel Cell-based Assay for the Detection of Murine type I IfN
Mariela Bollati-Fogolín, Werner Müller
10. Characterisation of the Induction of Cytochrome p450 Enzymes in Primary Cultures of Human Hepatocytes
T.L. Freeman, A.P. Chadwick, M.S. Lennard, C.M. Martin, R.G. Turcan, D. Blond
11. Effect of Culture Conditions on Glycosylation of Recombinant beta-Interferon in CHO Cells
Maureen Spearman, Jose Rodriguez, Norm Huzel, Kevin Sunley, Michael Butler
12. Induction of Caspase-3-dependent Apoptosis by Electrolyzed Reduced Water/platinum Nanoparticles in Cancer Cells
T. Hamasaki, S. Aramaki, T. Imada, K. Teruya, S. Kabayama, Y. Katakura, K. Otubo, S. Morisawa, S. Shirahata
13. Molecular Mechanism of TAK1-Induced Repression of hTERT Transcription
M. Maura, Y. Katakura, T. Miura, T. Fujiki, H. Shiraishi, S. Shirahata
14. Analysis of Genetic Parameters in Order to Get More Information on High Producing Recombinant CHO Cell Lines
Christine Lattenmayer, M. Loeschel, K. Schriebl, E. Trummer, K. Vorauer-Uhl, D. Mueller, H. Katinger, R. Kunert
15. Redox Regulation by Reduced Waters as Active Hydrogen Donors and Intracellular ROS Scavengers for Prevention of type 2 Diabetes
S. Shirahata, Y. Li, T. Hamasaki, Z. Gadek, K. Teruya, S. Kabayama, K. Otsubo, S. Morisawa, Y. Ishii, Y. Katakura
16. Transcription Profiling of Different Recombinant CHO Clones Based on Cross-species Microarray Analysis
Evelyn Trummer, Wolfgang Ernst, Dethardt Müller, Kornelia Schriebl, Christine Lattenmayer, Karola Vorauer-Uhl, Renate Kunert, Hermann Katinger, Friedemann Hesse
17. Adaptation without Cell Division?
K.L. Astley, M. Al-Rubeai
18. Release of Plasmid-DNC from PEI/DNA Particles
Martin Bertschinger, Arnaud Schertenleib, Gaurav Backliwal, Martin Jordan, Florian M. Wurm
19. Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody
Shinei Matsumoto, Yoshinori Katakura, Makiko Yamashita, Yoshihiro Aiba, Kiichiro Teruya, Sanetaka Shirahata
20. Design Approach Regarding Humanization and Functionality of an Anti-CD18 Monoclonal Antibody
Cristina A. Caldas, Diorge P. Souza, Maria Teresa A. Rodrigues, Andréa Q. Maranhão, Ana M. Moro, Marcelo M. Brigido
21. Development of a Transient Mammalian Expression Process for the Production of the Cancer Testis Antigen NY-ESO-1
David L. Hacker, Leonard Cohen, Natalie Muller, Huy-Phan Thanh, Elisabeth Derow, Gerd Ritter, Florian M. Wurm
22. The Role of the Sumoylation Pathway in Transient and Stable Recombinant Protein Expression in Chinese Hamster Ovary Cells
Martin Bertschinger, David Hacker, Florian Wurm
23. Effect of Different Materials Used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium
Peter Aizawa, Göran Karlsson, Charlotte Benemar, Vera Segl, Kristina Martinelle, Elisabeth Lindner
24. Antibody Production by GS-CHO Cell Lines Over Extended Culture Periods
A.J. Porter, A.J. Dickson, L.M. Barnes, A.J. Racher
25. Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells
Michael Schomberg, Christian Hakemeyer, Heino Büntemeyer, Lars Stiens, Jürgen Lehmann
26. Effect of the Scaffold in Maintenance of Suitable Chondrcyte Morphology in Cartilage Regeneration
J. Rodríguez, X. Rubiralta, J. Farré, P.J. Fabregues, J.M. Aguilera, J. Barrachina, F. Granell, J. Nebot, J.J. Cairó, F. Gòdia
27. Metabolic Changes Occuring During Adaptation to Serum Free Media
Sam Denby, Frank Baganz, David Mousdale
28. The Expression of the
Farlan Veraitch, Mohamed Al-Rubeai
CHAPTER III. : THERAPEUTIC CELL ENGINEERING
29. The Therapeutic Potential of Human Embryonic Stem Cells
S.H. Cedar, M.J. Patel, S.J. Pickering, P.R. Braude, S.L. Minger
30. Investigations of Murine Embryonic Stem Cell Maintenance by Analyses of Culture Variables and Gene Expression
James M. Piret, Clive H. Glover, Michael Marin, Mohammed A. S. Chaudhry, Connie J. Eaves, Bruce D. Bowen, R. Keith Humphries, Cheryl D. Helgason, Jennifer Bryan
31. Generation of Cardiomyocytes Derived from Mouse ESC in a Scale-up System
S. Niebruegge, H. Ebelt, M. Jungblut, H. Baer, M. Schroeder, R. Zweigerdt, T. Braun, J. Lehmann
32. Expansion of Undifferentiated Human Embryonic Stem Cells
Andre B.H. Choo, Jeremy Crook, Steve K.W. Oh
33. New Electrofusion Devices for the Improved Generation of Dendritic Cell-tumour Cell Hybrids
Janina Schaper, Hermann Richard Bohnenkamp, Thomas Noll
34. Influence of c-Myc and bcl-2 CO-expression on Proliferation, Apoptosis and Survival in CHO Cultures
Vasiliki Ifandi, Mohamed Al-Rubeai
35. Ex Vivo Expansion of Hematopoietic Stem Cells Using Defined Culture Media
J. Hartshorn, A.S.-H. Chan, Z.-H. Geng, L. Davis, S. Luo, J. Ni, K. Etchberger
36. Development of A Novel Serum-free Cryopreservative Solution
T. Toyosawa, M. Sasaki, Y. Kato, H. Yamada, S. Terada
37. Hepatic Cell Lines CultureD on Different Scaffolds and in Different Stages for Bioartificial Liver Systems
Kozue Kaito, Yumi Narita, Satoshi Terada
38. Comparison of Flask and Bag Systems in Cultivation of Dendritic Cells for Clinical Application
L. Macke, B. Wrobel, W. Meyring, H.S.P. Garritsen, B. Wörmann, M. Rhode, W. Lindenmaier, K.E.J. Dittmar
39. T-Lymphocytes Transduced with SFCMM-3 Vector in a Closed System
Claudia Benati, Roberto Sciarretta Birolo, Simona La Seta Catamancio, Marina Radrizzani, Cecilia Sendresen, Salvatore Toma
40. Influence of Down-regulation of Caspase-3 and CASPASE-7 by Sirnas on Sodium Butyrate-induced Apoptotic Cell Death of Chinese Hamster Ovary Cells Producing Thrombopoietin
Yun Hee Sung, Soon Hye Park, Sung Hyun Kim, Gyun Min Lee
CHAPTER IV. : GENE MEDICINE
41. UTRtech™: Exploiting mRNA Targeting To Increase Protein Secretion From Mammalian Cells
Beate Stern, Christine Gjerdrum, Stian Knappskog, Laura Minsaas, Stian Nylund, Lene C. Olsen, Litta Olsen, Eystein Oveland, Hanne Ravneberg, Christiane Trösse, Anja Tveit, Endre Vollsund, John E. Hesketh, Albert Tauler, Ian F. Pryme
42. Semi-Synthetic Mammalian Gene Regulatory Networks
B.P. Kramer, M. Fischer, M. Fussenegger
43. Acoustic Cell Processing for Viral Transduction or Bioreactor Cell Retention
V.M. Gorenflo, P. Beauchesne, V. Tayi, O. Lara, H. Drouin, J.B. Ritter, V. Chow, C. Sherwood, B.D. Bowen, J.M. Piret
44. Comparison of Different Transient Retroviral Transfection Systems
V. Kleff, I. Rattmann, C. Ludwig, M. Flasshove, T. Moritz, B. Opalka
CHAPTER V. : CELLS TO TISSUES
45. Artificial Human Lymphnode
C. Giese, U. Marx
46. Single Cell Study in a Hydrogel
T. Braschler, R. Johann, U. Seger, H. Van Lintel, P. Renaud
47. Predicting Tissue Assembly of Prostate Cancer Spheroids
Kim O’Connor, Cristina Vidulescu, Sanda Clejan, Hong Song, Mark Venczel
48. Cultivating Cells of Different Origin for 3d Bone Constructs Considering Physiological Conditions
K. Suck, C. Kasper, C. Hildebrandt, S. Diederichs, M. Fischer, T. Scheper, M. van Griensven
49. Guidance on Good Cell Culture Practice
Sandra Coecke, Michael Balls, Gerard Bowe, John Davis, Gerhard Gstraunthaler, Thomas Hartung, Robert Hay, Anna Price, Otto-Wilhelm Merten, William Stokes, Leonard Schechtman, Glyn Stacey
50. In Vitro Vascularization of Human Microtissues
J. M. Kelm, C. Diaz Sanchez-Bustamante, M. Fussenegger
51. Preparation of Salmon Atelocollagen Fibrillar Gel and Its Application to the Cell Culture
Nobuhiro Nagai, Shunji Yunoki, Takeshi Suzuki, Yasuharu Satoh, Kenji Tajima, Masanobu Munekata
52. Propagation of USSC for Therapeutic Approaches
J. Lamlé, H. Büntemeyer, A. Reinel, T. Menne, C. van den Bos, J. Lehmann
53. Bioreactor Based Production of Functional Conditioned Medium for the Propagation of Undifferentiated Human Embryonic Stem Cells
A. Bauwens, H. Büntemeyer, S. Terstegge, T. Nottorf, O. Brüstle, J. Lehmann
54. Large-scale Expansion of Cells for Use as Therapeutic Agents
Juan Melero-Martin, Shanmugapriya Santhalingam, Mohamed Al-Rubeai
55.
Stacy L. Leugers, Daniel W. Allison, Ying Liang, Carol Swiderski, Gary Van Zant, Laurel M. Donahue
56. In Vitro Bone Formation of Multipotential Human Mesenchymal Cells in Fixed Bed Perfusion Bioreactors
Nadia Zghoul, Martijn van Griensven, Johannes Zeichen, Kurt E.J. Dittmar, Manfred Rohde, Volker Jäger
57. Influence of Basal Media Formulations and Calcium on Growth and Directed Differentiation of Human Osteogenic Cells
Ingrida Majore, M. Morris Hosseini, Hubert Mayer, Volker Jäger
CHAPTER VI. : PROTEIN PRODUCTS
58. BHRF-1 as a Tool for Genetic Inhibition of Apoptotis in Hybridoma Cell Cultures
S. Juanola, J. Vives, J. Gálvez, P. Ferrer, R. Tello, J.J. Cairó, F. Gòdia
59. Karyotype of CHO DG44 cells
D. Martinet, M. Derouazi, N. Besuchet, M. Wicht, J. Beckmann, F.M. Wurm
60. From Biopsy to Cartilage-carrier-constructs by Using Microcarrier Cultures as Sub-process
Stephanie Nagel-Heyer, Svenja Lünse, Christian Leist, Roman Böer, Christiane Goepfert, Ralf Pörtne
61. Effect of Peptones and Study of Feeding Strategies in a CHO Based Fed-batch Process for the Production of a Human Monoclonal Antibody
Véronique Chotteau, Caroline Wåhlgren, Helena Pettersson
62. Retinoic Acid Induced Up-regulation of Monoclonal Antibody Production
Yuichi Inoue, Hiroharu Kawahara, Sanetaka Shirahata, Yasushi Sugimoto
63. Media and Cell Manipulation Approaches to the Reduction of Adaptation Time in GS NS0 Cell Line
Paul Clee, Mohamed Al-Rubeai
64. The Production of Human Growth Hormone
Fabienne Anton, Alexander Tappe, Cornelia Kasper, Alexander Loa, Bernd-Ulrich Wilhelm, Thomas Scheper
65. Preparation of Membrane Receptor Proteins Utilising Large Scale Micro-carrier Culture and a "Pearl Freezing Factory" to Finalise the Product
Irma Jansson, Thomas Fröberg, Anette Danielsson, Elke Lullau
66. Silk Protein Sericin Improves Mammalian Cell Culture
Satoshi Terada, Naoki Takada, Kazuaki Itoh, Takuya Saitoh, Masahiro Sasaki, Hideyuki Yamada
67. Design of Culture Media for Drosophila Melanogaster S2 Cells Producing Recombinant G Glycoprotein from Rabies Virus
A.L.L. Galesi, F.R.X. Batist, R.Z. Mendonça, C.A. Pereira, A.M. Moraes
68. Comparison of the Cultivation of Wild and Transfected
K. Swiech, A.L.L. Galesi, A.M. Moraes, R.Z. Mendonça, C.A. Pereira, C.A.T. Suazo
69. Low Temperature Culture Effects on sCR1 Productivity by rCHO Cells
Hiroshi Matsuoka, Chie Shimizu, Toshiya Takeda
70. Proteomic Characterization of Recombinant NS0 Cell Clones Obtained by an Integrated Selection Strategy
Adolfo Castillo, Kathya R. De La Luz, Svieta Victores, Luis Rojas, Yamilet Rabasa, Simon Gaskell, Rolando Perez
71. IFN-γ Glycosylation Macroheterogeneity and Intracellular Nucleotide and Sugar Nucleotide Contents of CHO Cells Cultivated in Batch Culture
N. Kochanowski, F. Blanchard, E. Guedon, A. Marc, R. Cacan, F. Chirat, J.-L. Goergen
72. Influence of the Adaptation Procedure and the Serum-free Conditions on the N-glycosylation Pattern of EPO Produced by CHO Cells
F. Le Floch, B. Tessier, J.-M. Guillaume, P. Cans, A. Marc, J.-L. Goergen
73. Stability and Cytogenetic Characterization of Recombinant CHO Cell Lines Established by Microinjection and Calcium Phosphate Transfection
M. Derouazi, D. Martinet, N. Besuchet, R. Flaction, M. Wicht, M. Bertschinger, D. Hacker, J. Beckmann, F.M. Wurm
74. Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody
Shinei Matsumoto, Yoshinori Katakura, Makiko Yamashita, Yoshihiro Aiba, Kiichiro Teruya, Sanetaka Shirahata
75. Design Approach Regarding Humanization and Functionality of an Anti-CD18 Monoclonal Antibody
Cristina A. Caldas, Diorge P. Souza, Maria Teresa A. Rodrigues, Andréa Q. Maranhão, Ana M. Moro, Marcelo M. Brigido
76. Development of a Transient Mammalian Expression Process for the Production of the Cancer Testis Antigen NY-ESO-1
David L. Hacker, Leonard Cohen, Natalie Muller, Huy-Phan Thanh, Elisabeth Derow, Gerd Ritter, Florian M. Wurm
77. Effect of Different Materials used in Bioreactor Equipments on Cell Growth of Human Embryonic Kidney (HEK293) Cells Cultivated in a Protein-free Medium
Peter Aizawa, Göran Karlsson, Charlotte Benemar, Vera Segl, Kristina Martinelle, Elisabeth Lindner
78. Antibody Production by GS-CHO Cell Lines Over Extended Culture Periods
A.J. Porter, A.J. Dickson, L.M. Barnes, A.J. Racher
79. Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells
Michael Schomberg, Christian Hakemeyer, Heino Büntemeyer, Lars Stiens, Jürgen Lehmann
80. Growth Promoting Effect of a Hydrophilic Fraction of the Protein Hydrolysate Primatone on Hybridoma Cells
Michael Schomberg, Christian Hakemeyer, Heino Büntemeyer, Lars Stiens, Jürgen Lehmann
81. Metabolic Changes Occuring During Adaptation to Serum Free Media
Sam Denby, Frank Baganz, David Mousdale
82. The Expression of the
Farlan Veraitch, Mohamed Al-Rubea
83. 1000 Non-instrumented Bioreactors in a Week
Matthieu Stettler, Maria De Jesus, Hajer Ouertatani-Sakouhi, Eva Maria Engelhardt, Natalie Muller, Sébastien Chenuet, Martin Bertschinger, Lucia Baldi, David Hacker, Martin Jordan, Florian M. Wurm
84. Optimisation of Time-space-yield for Hybridoma fed-batch Cultures with an Adaptive Olfo-controller
Ralf Pörtner, Björn Frahm, Paul Lane, Axel Munack, Kathrin Kühn, Volker C. Hass
85. Influenza Vaccines – Challenges in Mammalian Cell Culture Technology
Yvonne Genzel, Josef Schulze-Horsel, Lars Möhler, Yury Sidorenko, Udo Reichl
86. Towards pCO
Alexander Jockwer, Christian Klinger, Jochem Gätgens, Detlef Eisenkrätzer, Thomas Noll Gätgens
87. Cultivation of E-FL in Perfusion Mode using Wave
B. Hundt, S. Straube, N. Schlawin, H. Kaßner, U. Reichl
88. Repeated Batch Fermentation Using a Viafuge Centrifuge
Kurt Russ, Peter Schlenke, Harald Huchler, Hans Hofer, Maya Kuchenbecker, Ralf Fehrenbach
89. Serum-free Transient Expression of Antibodies Using 40 Tray Cell Factories
Peter Schlenke, Katrin Langnickel, Thilo Grob, Frank Kohne, Hans Hofer, Ulrike Brändle, Kurt Russ, Ralf Fehrenbach
90. Monitoring Cell Physiology in Influenza Vaccine Production by Flow Cytometry
Josef Schulze-Horsel, Yvonne Genzel, Udo Reichl
91. NucleoCounter – An Efficient Technique for the Determination of Cell Number and Viability in Animal Cell Culture Processes
Dimpalkumar Shah, Paul Clee, Sthen Boisen, Mohammed Al-Rubeai
92. On-line Biomass Monitoring in Cell Culture with Scanning Radio-frequency Impedance Spectroscopy
J.P. Carvell, C. Cannizzaro
93. The Use of a Mobile Robot for Complete Sample Management in a Cell Culture Pilot Plant
Martin Wojtczyk, Rüdiger Heidemann, Klaus Joeris, Chun Zhang, Mark Burnett, Alois Knoll, Konstantin Konstantinov
94. Closed Loop Control of Perfusion Systems in High-density Cell Culture
A. Bock, U. Reichl
95. Optimization of Culture Medium with the Use of Protein Hydrolysates
Paul Ganglberger, Bernd Obermüller, Manuela Kainer, Peter Hinterleitner, Otto Doblhoff, Karlheinz Landauer
96. Optimization of a Fed-batch Strategy for Production of Mab Ign 311 in Small Scale Experiments
P. Hinterleitner, P. Ganglberger, B. Obermüller, B. Antes, O. Doblhoff, K. Landauer
97. Serum-free Influenza Vaccine Production with MDCK Cells in Wave-bioreactor and 5L-stirred Tank Bioreactor
Yvonne Genzel, Marlies Fischer, Ruth Maria Olmer, Bastian Schäfer, Claudia Best, Susanne König, Boris Hundt, Udo Reichl
98. Rotary Bioreactor for Recombinant Protein Production
Stephen Navran
99. Flow Cytometry as a Tool for Process Monitoring of Virus-cell Systems
K.S. Sandhu, M. Al-Rubeai
100. The Use of Multi-parameter Flow Cytometry for Characterisation of Insect Cell-baculovirus Cultures
B. Isailovic, R. Hicks, I.W. Taylor, A. Nienow, C.J. Hewitt
101. Understanding Metabolic Needs of EB14 Cells in Culture
J. Hartshorn, F. Guehenneux, K. Kolell, Z.-H. Geng, E. Atarod, T. Mohabbat, C. McCormick, P. Sutrave, C. Hernandez, M. Avery, S. Luo, M. Mehtali, K. Etchberger
102. Virus Production in Vero Cells Using a Serum-free Medium
K. Kolell, J. Padilla-Zamudio, B. Schuchhardt, S. Gilliland, S. McNorton, B. Dalton, S. Luo, K. Etchberger
103. Computational Fluid Dynamics Applied to Laboratory Scale Cell Culture Reactors for the Prediction of Hydrodynamic Stress
Eric Olmos, Nicolas Fischbach, Annie Marc
104. Using Genomic Tools for the Identification of Important Signaling Pathways in Order to Facilitate Cell Culture Medium Development
Daniel W. Allison, Kathryn A. Aboytes, Terrell K. Johnson, Danny K. Fong, Stacy L. Leugers, Melissa L. Hoepner, Laurel M. Donahue
105. Development and Application of an Animalcomponent Free Single-cell Cloning Medium for Chinese Hamster Ovary Cell Lines
N. Lin, J. L. Beckmann, J. Cresswell, J. S. Ross, B. Delong, Z. Deeds, M. V. Caple
106. Plant Peptones: Nutritional Properties Sustaining Recombinant Protein Secretion by CHO Cells
Johann Mols, Nicolas Cavez, Spiros Agathos, Yves-Jacques Schneider
107. Monitoring of Extracellular TCA Cycle Intermediates in Mammalian Cell Culture
Joachim B. Ritter, Yvonne Genzel, Udo Reichl
108. A Comparison of the SixFors Fermenter System and Lab Scale Fermenters For Clone Selection
Giles Wilson, Annette Jørgensen
109. Comparison of Cultivations of CHO Cell Line in Chemostat and Acceleratostat Experiments
Rasmus Bjerre Nielsen, Giles Wilson
110. Evaluation of a Novel Micro-bioreactor System for Cell Culture Optimisation
Giles Wilson
111. Comparison of Different Design Configurations of a 1-L Multi-bioreactor Station for Cell Culture Process Development
Heiko Schütte, Ulrike Kolrep, Liane Franck, Roland Wagner
112. The Effect of Genetically Modified Insulin Growth Factor on Cell Proliferation and Adenovirus Productivity in Serum Free Medium
Angela Buckler, Mohamed Al-Rubeai
113. Evaluation of a Scalable Disposable Bioreactor System for Manufacturing of Mammalian Cell Based Biopharmaceuticals
Silke Langhammer, Marco Riedel, Hikmat Bushnaq-Josting, René Brecht, Ralf Pörtner, Uwe Marx
114. Purification of Rabies Virus Produced on Vero Cells Using Chromatography Techniques
Mohamed Chtioui, Khaled Trabelsi, Héla Kallel
115. Human Growth Hormone (HGH) Purification From CHO-Cell Culture Supernatant Utilizing Macroporous Chromatographic Media
Johanna-Gabriela Walter, Alexander Tappe, Cornelia Kasper, Robert Zeidler, Oscar-Werner Reif, Thomas Scheper
116. Process Development for a Veterinary Vaccine Against Heartwater Using Stirred Tanks
Isabel Marcelino, Marcos F.Q. Sousa, Ana Amaral, Cristina Peixoto, Célia Verissimo, Antonio Cunha, Manuel J.T. Carrondo, Paula M. Alves
117. Working Towards a Chemically-defined Replacement for Hydrolysates
Zachary W. Deeds, C. Steven Updike, Benjamin J. Cutak, Matthew V. Caple
118. Effect of Vitamins or Lipids Addition on Adenovirus Production at High Cell Densities
Tiago B. Ferreira, Manuel J. T. Carrondo, Paula M. Alves
119. Observation and Analysis of Lab Scaled Microcarrier Cultivation by In-situ Microscopy with Image Processing Tools
G. Rudolph, A. Gierse, P. Lindner, C. Kasper, B. Hitzmann, T. Scheper
120. Rapeseed Peptidic Fractions as Medium Additives in Animal Cell Bioreactors
B. Farges, B. Tessier, I. Chevalot, C. Harscoat, S. Chenu, I. Marc, J.L. Goergen, A. Marc
121. Influence of Rapeseed Protein Hydrolysis Conditions on Animal Cell Growth in Serum-free Media Supplemented with Hydrolysates
G. Chabanon, I. Chevalot, B. Farges, C. Harscoat, S. Chenu, J.-L. Goergen, A. Marc, I. Marc
122. Rapid Media and Process Optimization Using DOE: A Case Study
Stacy Holdread, Ji Hee Kim, Toyin Oshunwusi, Jagdish Kuchibhatla, Cindy Hunt, James W. Brooks
123. Developing Chemically Defined Media Through DOE: Complete Optimization with Increased Protein Production in Less than 8 Months
Kimesha Hammett, Jagdish Kuchibhatla, Cindy Hunt, Stacy Holdread, James W. Brooks
124. Bioreactor Process Strategies for rNS0 Cells
Delia Fernandez, Jennifer Walowitz, William Paul, Lia Tescione, Stephen Gorfien
125. The Efficient Induction of Human Antibody Production in
Yoshihiro Aiba, Makiko Yamashita, Yoshinori Katakura, Sin-ei Matsumoto, Kiichiro Teruya, Sanetaka Shirahata
126. Rapid Culture Media Optimization to Gain Multiple Fold Increase in Recombinant Protein Production in Two CHO Cell Lines
Scott D Storms, Aaron Chen, Jenny Yu, Tom Fletcher
127. Strategies for Large Scale Production of
M. Pohlscheidt, U. Langer, B. Bödeker, D. Paulsen, H. Rübsamen-Waigmann, U. Reichl, H.J. Henzler, H. Apeler
128. Proteomic Investigation of Recombinant CHO Cells in High Density Culture
J.R. Cresswell, J.M. Zobrist, J. Wildsmith, N. Lin, R. Valdes-Camin, M. Caple
129. Comparative Proteomic Study of Mechanisms Involved in the Expression of Recombinant Monoclonal Antibody in Non Amplified NS0 Myeloma Cell Line
Luis E. Rojas, Kathya R. de la Luz Hernández, Svieta Victores, Lila Castellanos, Simon Gaskell, Adolfo Castillo, Rolando Pérez
130. Monitoring Nutrient Limitations by Online Capacitance Measurements in Batch & Fed-batch CHO Fermentations
Sven Ansorge, Geoffrey Esteban, Charles Ghommidh, Georg Schmid
131. Characterisation of Shake Flasks for Cultivation of Animal Cell Cultures
G. Jänicke, C. Sauter, R. Bux, J. Haas
132. Manufacturing of Lots of Human T-Lymphocytes for Cell Therapy
Nadia De Bernardi, Silvia Trasciatti, Antonio Orlandi, Fausto Gaspari, Ilaria Giuntini, Tiziana Bacchi, Sonia Castiglioni, Elena Muru, Marta Galagano, Claudia De Mattei, Luigi Cavenaghi, Maria Luisa Nolli
133. Oxygen Control in Static Cell Cultures
Nadia De Bernardi, Edwin Schwander, Antonio Orlandi, Ilaria Tano, Sonia Castiglioni, Elena Muru, Fausto Gaspari, Marta Galgano, Claudia De Mattei, Luigi Cavenaghi, Maria Luisa Nolli
134. The Effects of Cell Separation with Hydrocyclones on the Viability of CHO Cells
Rodrigo C. V. Pinto, Ricardo A. Medronho, Leda R. Castilho
135. Erythropoietin Purification Employing Affinity Membrane Adsorbers
Maria Candida M. Mellado, David Curbelo, Ronaldo Nobrega, Leda R. Castilho
136. Cultivation of Vero Cells on Microporous and Macroporous Microcarriers
Marta Cristina O. Souza, Marcos S. Freire, Leda R. Castilho
137. CFD-Aided Design of Hollow Fibre Modules for Integrated Mammalian Cell Retention and Product Purification
Romi L. Machado, Alvio Figueredo, Danilo G. P. Carneiro, Leda R. Castilho, Ricardo A. Medronho
138. Comparison of Manufacture Technologies for RAV12 Monoclonal Antibody in Production Medium Containing No Animal Derived Proteins
Irene Shackel, Amy Bass, Arno Brewer, Peter Brown, Mary C. Tsao, Lucille W.S. Chang
139. Highly Efficient Serum-free Production of Biopharmaceuticals in CHO-Cells
S. Zahn, K. Abst, N. Palmen, S. Schindler, A. Herrmann
140. A New Host Cell-line for the Fast, Reproducible and Efficient Production of Biopharmaceuticals
B. Greulich, H.F. Abts, S. Zahn, A. Herrmann
141. Proteomic Characterization of Recombinant NS0 Cell Clones Obtained by an Integrated Selection Strategy
Adolfo Castillo, Kathya r. De La Luz, Svieta Victores, Luis Rojas, Yamilet Rabasa, Simon Gaskell, Rolando Perez
142. Analysis and Modeling of the Metabolism of Lepidopteran Cell Lines
Jean-Christophe Drugmand, Yves-Jaques Schneider, Spiros Agathos
143. Growth of Mammalian and Lepidopteran Cells on BioNOC® II Disks, a Novel Macroporous Microcarrier
Jean-Christophe Drugmand, Jean-François Michiels J.-F., Spiros Agathos, Yves-Jaques Schneider
144. Screening Strategies for Iron Chelators in Serum Free Media
Joanne Keenan, Dermot Pearson, Martin Clynes
145. Production of Human Growth Hormone in a Mammalian Cell High Density Perfusion Process
T. Nottorf, W. Hoera, H. Buentemeyer, S. Siwiora-Brenke, A. Loa, J. Lehmann
146. Development of an Integrated Qualitative and Quantitative Approach to Study Expression- Secretion Bottlenecks of Mammalian cDNAs in HEK293 EBNA Cells
Loïc Glez, Christine A. Power, Thierry Battle, Georg Feger
147. Rotating Bed Bioreactors: a Novel Way to Natural Proteins by Tissue-like Cultivation of Mammalian Cells
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148. Minireactors for Animal Cell Culture: Monitoring of Cell Concentration and Cellular Activity
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Avainsanat: Chemistry, Biotechnology
- Tekijä(t)
- Smith, Rodney
- Julkaisija
- Springer
- Julkaisuvuosi
- 2007
- Kieli
- en
- Painos
- 1
- Sivumäärä
- 900 sivua
- Kategoria
- Eksaktit luonnontieteet
- Tiedostomuoto
- E-kirja
- eISBN (PDF)
- 9781402054761