Makkar, Harinder P.S.
Methods in Gut Microbial Ecology for Ruminants
Part One. Designing **in vivo microbial ecology studies
1. Experimental designs for rumen microbiology
Adrian R. Egan
Part Two. Classical methods for isolation, enumeration, cultivation and functional assays of rumen microbes
2. Rumen bacteria
Christopher S. McSweeney, Stuart E. Denman, Roderick I. Mackie
3. Bacteriophages
Athol V. Klieve
4. Methanogenic archaea
Keith N. Joblin
5. Anaerobic fungi
Michael K. Theodorou, Jayne Brookman, Anthony P.J. Trinci
6. Ciliate protozoa
Burk A. Dehority
Part Three. PCR-based methods for analysis of populations and gene expression
7. Nucleic acid extraction, oligonucleotide probes and PCR methods
Zhongtang Yu, Robert J. Forster
8. Quantitative (real-time) PCR
Stuart E. Denman, Christopher S. McSweeney
Part Four. Molecular fingerprinting techniques for genotypic analysis of pure cultures and microbial communities
9. Denaturing gradient gel electrophoresis
Svetlana A. Kocherginskaya, Isaac K.O. Cann, Roderick I. Mackie
10. Bacteriophage populations
Athol V. Klieve, Rosalind A. Gilbert
11. Anaerobic fungal populations
Jayne L. Brookman, Matthew J. Nicholson
12. RAPD, RFLP, T-RFLP, AFLP, RISA
Stuart E. Denman, Makoto Mitsumori, Christopher S. Mcsweeney
Part Five. DNA clone libraries of microbial communities
13. 16S/18S ribosomal DNA clone library analysis of rumen microbial diversity
André-Denis G. Wright, Kiyoshi Tajima, Rustam I. Aminov
Part Six. Use of small subunit ribosomal RNA directed oligonucleotide probes for microbial population studies
14. Northern blot analysis to investigate the abundance of microorganisms
Denis O. Krause
15. Whole cell probing with fluorescently labelled probes for
Linda L. Blackall
16. Combined fluorescence
Maneesha P. Ginige
Part Seven. Genomic analysis of microbial ecosystems
17. Metagenomic analysis of the microbiomes in ruminants and other herbivores
Mark Morrison, Sarah E. Adams, Karen E. Nelson, Graeme T. Attwood
DRM-restrictions
Printing: not available
Clipboard copying: not available
Avainsanat: SCIENCE / Life Sciences / Biology / General SCI008000
- Tekijä(t)
- Makkar, Harinder P.S.
- McSweeney, Christopher S.
- Julkaisija
- Springer
- Julkaisuvuosi
- 2005
- Kieli
- en
- Painos
- 1
- Kategoria
- Eksaktit luonnontieteet
- Tiedostomuoto
- E-kirja
- eISBN (PDF)
- 9781402037917