Transgenic Ascidians

1. Introduction
Yasunori Sasakura

2. Microinjection of Exogenous Nucleic Acids into Eggs: Ciona Species
Kenji Kobayashi, Yutaka Satou

3. Practical Guide for Ascidian Microinjection: Phallusia mammillata

Hitoyoshi Yasuo, Alex McDougall

4. Microinjection of Exogenous DNA into Eggs of Halocynthia roretzi

Gaku Kumano

5. Electroporation in Ascidians: History, Theory and Protocols
Robert W. Zeller

6. The Use of cis-Regulatory DNAs as Molecular Tools
Kotaro Shimai, Takehiro G. Kusakabe

7. Reporter Analyses Reveal Redundant Enhancers that Confer Robustness on Cis-Regulatory Mechanisms
Shigeki Fujiwara, Cristian Cañestro

8. Investigating Evolutionarily Conserved Molecular Mechanisms Controlling Gene Expression in the Notochord
Julie E. Maguire, Aakarsha Pandey, Yushi Wu, Anna Di Gregorio

9. Purification of Fluorescent Labeled Cells from Dissociated Ciona Embryos
Wei Wang, Claudia Racioppi, Basile Gravez, Lionel Christiaen

10. Germline Transgenesis in Ciona

Yasunori Sasakura

11. The Enhancer Trap in Ciona

Yasunori Sasakura

12. TALEN-Based Knockout System
Keita Yoshida, Nicholas Treen

13. CRISPR Knockouts in Ciona Embryos
Shashank Gandhi, Florian Razy-Krajka, Lionel Christiaen, Alberto Stolfi

14. Transgenic Techniques for Investigating Cell Biology During Development
Christina D. Cota

15. Cellular Processes of Notochord Formation
William C. Smith

16. Morphology and Physiology of the Ascidian Nervous Systems and the Effectors
Atsuo Nishino

Keywords: Life Sciences, Developmental Biology, Transgenics, Cell Biology, Biological Techniques, Evolutionary Biology