Protocols in Semen Biology (Comparing Assays)

1. Semen Analysis: An Overview
N. Srivastava, Megha Pande

2. Selection of Spermatozoa
N. Srivastava, Megha Pande, S. Tyagi, Omer Din

3. Handling and Applications of Optical Devices
Praveen Singh

4. Basic Semen Assays
Megha Pande, N. Srivastava, Sarvesh Arya

5. Estimates of Sperm Motility
N. Srivastava, Megha Pande

6. Evaluating Sperm Cell Viability and Membrane Integrity
Pradeep Kumar, N. Srivastava, Megha Pande, J. K. Prasad, A. S. Sirohi

7. Counting Sperm Numbers
P. Perumal, N. Srivastava, Megha Pande, S. K. Ghosh

8. Evaluating Sperm Cell Morphology
N. Srivastava, Megha Pande, Omer Din

9. Biochemical Assays in Spermatology
N. Srivastava, Megha Pande

10. Estimating Metabolic Activity of Spermatozoa
N. Srivastava, Megha Pande

11. Evaluating Resistance of Spermatozoa to Adverse Conditions
N. Srivastava, Megha Pande

12. Determining Oxidative Stress of Spermatozoa
Megha Pande, N. Srivastava

13. Determination of Mitochondrial Function in Sperm Cells
N. Srivastava, Megha Pande

14. Determination of Capacitation-Related Changes Using Fluorescent Stains
N. Srivastava, Megha Pande

15. Evaluating Damages to Sperm DNA
N. Srivastava, Megha Pande

16. Estimating Proteins of Semen
N. Srivastava, Megha Pande

17. Measuring Lipids of Spermatozoa and Seminal Plasma
N. Srivastava, Megha Pande

18. Gel Electrophoresis of Proteins and Nucleic Acids
Ashok K. Mohanty, Munna Lal Yadav, Suman Choudhary

19. Immuno-Reproduction Assays in Semen Biology
N. Srivastava, Megha Pande, Omer Din

20. Sperm Function Assays
Sadhan Bag, P. S. Mahapatra, Purnima Singh

Keywords: Life Sciences, Biological Techniques, Animal Physiology, Veterinary Medicine/Veterinary Science