Jenkins, Nigel

Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009

Jenkins, Nigel - Proceedings of the 21st Annual Meeting of the European Society for Animal Cell Technology (ESACT), Dublin, Ireland, June 7-10, 2009, ebook


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Table of contents

1. MicroRNAs as Potential Engineering Targets for Improvement of CHO Cell Production Phenotypes
Niall Barron, Niraj Kumar, Noelia Sanchez, Patrick Gammell, Martin Clynes

2. Are Clones Really Unstable?
Kriti Shukla, Saravanan Desan, Ankur Bhatnagar, Anuj Goel

3. An Optimised Transfection Platform for the Epi-CHO Transient Expression System in Serum-free Media
Joe Codamo, Trent P. Munro, Benjamin S. Hughes, Michael Song, Peter P. Gray

4. Using the “OMICS” Technologies as Complementary Tools to Study the Molecular Mechanisms Involved with the Adaptation of Myeloma Cell Line to Protein-Free Medium
K.R. Luz-Hernández, Y. Rabasa-Legón, A. Lage-Castellanos, A. Castillo-Vitlloch, L. Castellanos-Serra, J. Díaz-Brito, S. Gaskell

5. Galectin-3 Over-Expression Enhances Survival and Recombinant Protein Expression in Mammalian Cells
Fanny Delegrange, Mattia Matasci, Lucia Baldi, Florian M. Wurm

6. Metabolomic Analysis of CHO Cultures with Different Growth Characteristics – Development of a Metabolite Extraction Protocol for Suspension Adapted Mammalian Cells
Stefanie Dietmair, Nicholas E. Timmins, Panagiotis Chrysanthopoulos, Peter P. Gray, Jens O. Krömer, Lars K. Nielsen

7. Cell Lines in Four Weeks with the CEMAX® System
Benedikt Greulich, Karlheinz Landauer, Andreas Herrmann

8. Analysis of Protein Expression via Alternate 3’ Untranslated Region (UTR) Signals Through the Use of Site Specific Recombination
Jeff Jia Cheng Hou, Michael Song, Trent P. Munro, Peter P. Gray

9. A Case Study in Clone Screening: A Comprehensive Approach for a Product With High Projected Market Demand
Kirin M. Jamison, Dacia R. Brooks, Szu-Han Wang, Martin Gawlitzek

10. An Improved Clone Selection Method
Uros Jamnikar, Marjanca Blas, Dominik Gaser, Jerica Rozman-Pungercar, Andrej Francky

11. Dissecting the Mechanism of Action of BHRF1 for the Protection Against Apoptosis in MAb-Producing Cell Lines
Ernest Milián, Sandra Juanola, Eva Prats, Jordi J. Cairó, Francesc Gòdia, Joaquim Vives

12. Qualification of Bench and Pilot-Scale Bioreactors as Models of Commercial Systems
Anne Marie Molloy, Patrick Dowling, Mairead Looby, Caitriona Crawford, Bruce Tangarone, Mary Heenan, Enda Moran

13. Quantification of Polyethylenimine in Transient Gene Expression: On the Way to GMP Compliance
Sophie Nallet, Zuzana Kadlecova, Lucia Baldi, Harm-Anton Klok, Florian M. Wurm

14. Implications of Vector Fragmentation and Initial Production Levels in Production Cell Line Development Using MTX
Say Kong Ng, Wenyu Lin, Rohit Sachdeva, Daniel I.C. Wang, Miranda G.S. Yap

15. Characterizing the Expression Stability in Different Phenotypes of Recombinant NS0 Myeloma Cell Lines
Y. Prieto, L. Rojas, L. Hinojosa, K.R. Luz-Hernández, D. Aguiar, S. Victores, E. Faife, R. Pérez, A.J. Castillo

16. A Transient Gene Expression Process with Recombinant Antibody Titer of 0.5g/L in CHO Cells
Yashas Rajendra, Gaurav Backliwal, Markus Hildinger, Sarah Wulhfard, Maria Jesus, Florian M. Wurm

17. Proteomic Profiling of Temperature-Shifted CHO Cells to Identify Genes that Impact on Both Cell Growth and Recombinant Protein Productivity in Suspension Culture
Niraj Kumar, Noelia Sanchez, Patrick Gammell, Patrick Dowling, M. Henry, P. Meleady, Niall Barron, Martin Clynes

18. Stabilization of Protein Expression in Mammalian Cells Employing a Toxin/Antitoxin Based Strategy
Kristina Nehlsen, Jeannette Zauers, Sabrina Herrmann, Hansjörg Hauser, Dagmar Wirth

19. Novel Cell Lines For Bioprocessing: Friend or Foe?
Paula Ravnikar, Xiaoguang (Margaret) Liu, Jian Liu, Tasha Williams-Wright, Florence Wu

20. Transgene mRNA Levels and Stability are Key Factors to Enhance Transient Gene Expression in CHO DG44 Cells
Sarah Wulhfard, Divor Kiseljak, Lucia Baldi, David L. Hacker, Florian M. Wurm

21. High Cell Density Transient Gene Expression in HEK 293 EBNA Cells
Divor Kiseljak, Gaurav Backliwal, David L. Hacker, Lucia Baldi, Florian M. Wurm

22. Generation of High-Producing CHO Cell Lines by Piggybac Transposition
Mattia Matasci, Virginie Bachmann, Fanny Delegrange, Sebastien Chenuet, David L. Hacker, Florian M. Wurm

23. Cellular Proteins in Conditioned Medium Inhibit Polyethylenimine-Mediated Transfection of CHO Cells
Ralph Duhr, Divor Kiseljak, Yashas Rajendra, Lucia Baldi, David L. Hacker, Florian M. Wurm

24. A New Variant of the Affinity Matrix Method for Identification of High Producing Cells in Mammalian Cell Culture
Daniel Landgrebe, Larissa Behr, Pierre Moretti, Johanna Walter, Frank Stahl, Cornelia Kasper, Thomas Scheper

25. Novel Strategies for Improving Cell Viability and Production Yield
Hisahiro Tabuchi, Tomoya Sugiyama, Satoshi Tainaka

26. Regulating in Vitro Motility of Human Mesenchymal Stem Cells with Macrophage Migration Inhibitory Factor (MIF) and a Small-Molecule MIF Antagonist
Kim C. O’Connor, Bonnie L. Barrilleaux, Donald G. Phinney, Benjamin W. Fischer-Valuck, Katie C. Russell, Darwin J. Prockop

27. Impact of Growth Factors on the Proliferation of Ear Mesenchymal Stem Cells on Porous Microcarriers
Sébastien Sart, Yves-Jacques Schneider, Spiros N. Agathos

28. Strategies for Pancreatic Differentiation of Pluripotent Stem Cells
Insa S. Schroeder, Anna Daniel-Wojcik, Anna M. Wobus

29. Stem Cell Biology: New Applications for Studying Metabolic Diseases
Malte Sgodda, Reto Eggenschwiler, Tobias Cantz

30. Monitoring of Long-Term Cultivation and Osteogenic Differentiation of Human Umbilical Cord-Derived Mesenchymal Stem Cell-Like Cultures
P. Moretti, T. Hatlapatka, Magda Tomala, I. Majore, R. Hass, Thomas Scheper, C. Kasper

31. cGMP-Compliant Isolation, Expansion and Quality Testing of Human Bone Marrow-Derived Mesenchymal Stem Cells
Luca Romagnoli, Ilaria Giuntini, Marta Galgano, Chiara Crosta, Enrico Lucarelli, Davide Donati, Luigi Cavenaghi, Maria Luisa Nolli

32. Cancer Research, Molecular and Cell Biology: Look at the Nanotechnology, Based Nanoparticles for Diagnostics, and Therapy. Stem Cell for Treatment of Cancer Diseases
Katya Marinova Simeonova

33. A Comparative Study of Suspension Cultivation Systems for the Expansion of Undifferentiated Mouse Embryonic Stem Cells
Magda Tomala, Sabrina Schmeckebier, Ruth Olmer, Pierre Moretti, Frank Stahl, Ulrich Martin, Thomas Scheper, Cornelia Kasper

34. Human Endothelial Cell Lines with In Vivo Physiology
Tobias May, Milada Butueva, Sara Bantner, Herbert Weich, Hansjörg Hauser, Dagmar Wirth

35. Quality Control and Cell Line Cross-Contamination: An Old Problem that Continues To Fester
John R. Masters

36. Functional Characterisation of Human Hepatoma Cell Line HepG2 in 3D Culture
Christiane Goepfert, Wibke Scheurer, Susanne Rohn, Britta Rathjen, Hans Hoffmeister, Ralf Pörtner

37. GFP-Expressing Bladder Fibroblasts for Applications in Tissue Engineering
Eva-Maria Engelhardt, Agata Oberbek, Mattia Matasci, David L. Hacker, Myriam Adam, Florian M. Wurm

38. 3-Dimensional, Dynamic Cultivations of Human Umbilical Cord-Derived Cells
Dana Maria Marten, Stefanie Röker, Stefanie Böhm, Hans Hoffmeister, Cornelia Kasper

39. NIH/3T3 Feeder System to Engineer Corneal Epithelial Sheets with Enhanced Positive Progenitor Populations
Kishore Katiki Reddy, Martin Clynes, William Power, Andra Bobart, Finbarr O’Sullivan

40. NF-κB Signalling Pathway: Generation and Characterization of a Reporter Cell Line of Human Origin
Inés Tiscornia, Pablo Espósito, Valentina Porro, Paola Hernández, Hugo Cerecetto, Mercedes González, Eliezer Barreiro, Mariela Bollati-Fogolín

41. Establishing Mammalian Production Cell Lines for Structural Biology by Site-Specific Recombination
Sonja Wilke, Sarah Tokarski, Volker Jäger, Joop Heuvel, Manfred Gossen, Ermanno Gherardi, Konrad Büssow

42. Regulation of Transferrin Receptor and IGF-I Receptor Numbers at the Cell Surface Drives Growth and Productivity of Hybridoma Cells
Andrew J. Sakko, Kenneth C. Bertram, Sally Grosvenor, Collette Sheahan, Danny Voorhamme, Anthony Simula, Geoffrey L. Francis

43. Towards Enhancing Manufacturing Process Performance Through Multivariate Data Mining
Salim Charaniya, Huong Le, Keri Mills, Kevin Johnson, George Karypis, Wei-Shou Hu

44. Partial Replacement of Chemically Defined CHO Media with Plant-Derived Protein Hydrolysates
James F. Babcock, Amy Antosh

45. An Insight into the Physiology of Insect Cells: The Role of Energetic Metabolism on the Cell Density Effect
Vicente Bernal, Nuno Carinhas, Francisca Monteiro, Raquel Ambrósio, Manuel J.T. Carrondo, Paula M. Alves

46. Design and Development of a High-Throughput Platform for Rapid Microbe Identification and Automatic Data Management
Björn Breth

47. On-line Monitoring of the Live Cell Concentration in Disposable Bioreactors
J.P. Carvell, J. Williams, M. Lee, D. Logan

48. The Challenge of Validating a Viable Biomass Probe for cGMP Processes
J.P. Carvell, J. Williams, S. Taylor, M. Lee, R.W. Todd

49. Study of the Effect of High pH and Alkali Addition in a Cultivation of Chinese Hamster Ovary Cell
Véronique Chotteau, Anna Lindqvist

50. Tuning of Dissolved Oxygen and pH PID Control Parameters in Large Scale Bioreactor by Lag Control
Véronique Chotteau, Håkan Hjalmarsson

51. Kinetic Analysis of Recombinant BHK Roller Bottle Cultures
Frank Deer, Jennifer Mahoney, Abner Correia, Kevin Stafford

52. Manipulation of a Perfusion Process by Medium Optimization
Saravanan Desan, Omkar Nandi, Ankur Bhatnagar, Anuj Goel

53. Feed Flow Pulsation in the Separation of CHO Cells in Hydrocyclones: Effects of Pressure Drop and Pumphead Type on Separation Efficiency and Cell Viability
Elsayed A. Elsayed, Leonardo A.G. Ramalho, Leda R. Castilho, Ricardo A. Medronho

54. Development of a Biphasic Culture Process for Recombinant Protein Production in Human CAP Cells
Ruth Essers, Helmut Kewes, Gudrun Schiedner

55. Crossed Mixture Design and Artificial Neural Networks: An Efficient Approach to Cell Culture Medium Optimization
Guillermina Forno, Caroline Didier, Marina Etcheverrigaray, Héctor Goicoechea, Ricardo Kratje

56. Improving Cell Culture Bioreactor Performance for Sensitive Cell Lines by Dynamic Membrane Aeration (DMA)
B. Frahm, H. Brod

57. Evaluation of Disposable Bioreactor Design Compared to Reusable Stainless Steel Reactors
Gerhard Greller, Ute Noack

58. Utilising Scale Model Systems to Optimise Upstream Process Development
Sally Grosvenor, Larissa Chirkova, Tatyana Mitina, Danny Voorhamme, Quang Doan, Kenneth C. Bertram

59. Human Platelet Lysates as a Serum Substitute in Cell Culture Media
Caroline Rauch, Elisabeth Feifel, Hans Peter Spötl, Eva-Maria Amann, Harald Schennach, Harald Schöffl, Walter Pfaller, Gerhard Gstraunthaler

60. 4g/L.day: Monoclonal Antibody Volumetric Productivity in the iCELLisTM Disposable Fixed-Bed Bioreactor
Jean-Christophe Drugmand, Nicolas Havelange, Florence Collignon, José Castillo, P.-A. Girod

61. Optimisation of Cell Growth and Recombinant Protein Production in Small-Scale Culture Vessels by Using Optical Sensors for On-line Measurement of Dissolved Oxygen
Volker Jäger, Giannini Apati, Nadine Konisch

62. A Protocol for Cell Detachment of Vero Cells Grown Under Fully Animal Component Free Conditions and on Cytodex 1 Microcarriers
Samia Rourou, Nesrine Riahi, Héla Kallel

63. Simulation and Optimization of Essential Amino Acids in Dynamic Mammalian Cell Culture
Carolyn M. C. Lam, Alexandros Kiparissides, Danny C.F. Wong, Miranda G.S. Yap, Efstratios N. Pistikopoulos, Athanasios Mantalaris

64. Biomass Sensors in iCELLis™ Fixed-Bed Reactors: Data on CHO and Vero Cells
Jean -Christophe Drugmand, Florence Collignon, Stéphanie Dubois, Nicolas Havelange, José Castillo

65. On-line Monitoring: Animal Cell Cultivation in iCELLis™ Fixed-Bed Reactor Using Dielectric Measurements
Jean -Christophe Drugmand, Geoffrey Esteban, Naima Alaoui, Nadia Jafâr, Nicolas Havelange, Olivier Berteau, José Castillo

66. Change of the Role for JAK/STAT3 in IL-6 Response During Adaptation of Hybridoma Cells to Serum-Free Media
Ken Fukumoto, Masato Tanaka, Tatsuya Yamashita, Satoshi Terada

67. Engineering Principles and Cell Culture Performance of Orbitally Shaken Cylindrical Bioreactors
S. Tissot, X. Zhang, M. Stettler, D. Sanctis, M. Perone, C. Bürki, M. Jesus, M. Farhat, M. Discacciati, O.W. Reif, M. Kühner, Lucia Baldi, David L. Hacker, A. Quarteroni, Florian M. Wurm

68. Dynamic Ranking of Clones with Process Conditions Using a High-Throughput Micro-bioreactor Platform
Rachel Legmann, Brian Benoit, Cynthia Deppeler, Erwin Yu, Sriram Srinivasan, Ronald Fedechko, Russell Robins, David Ferrick, Ellen McCormick, Seth Rodgers, A. Peter Russo

69. Improved Cell Culture Surface for Biotechnological Research
Lara Breth

70. Platform Validation of Dissolved Oxygen Ranges for Cell Culture Processes
Nathan McKnight, Steven Meier, Srikanth Chary, Robert Kiss

71. Study of Growth Factors in Spent Medium for Better Mammalian Cell Culture
Akiko Ogawa, Sadaharu Fukui, Satoshi Terada

72. Process Improvements for Production of a Complex Glycosylated Fusion Protein which Delivers a Two-Fold Increase in Product Titre and Comparable Product Quality
Eimear O’Donovan, Patrick Gammell, Mairead Looby, Neysi Ibarra, Enda Moran

73. Hydro-Kinetic Modelling of Animal Cell Response to Turbulent Mixing in Bioreactors
E. Olmos, N. Barbouche, F. Fournier, Fabrice Blanchard, E. Guedon, Annie Marc

74. Near Infrared Spectroscopy as an In-Situ PAT Tool to Monitor Adherent VERO Cell Culture Processes
Emma Petiot, Patrick Bernard-Moulin, Cécile Gény, Thierry Magadoux, Hervé Pinton, Annie Marc

75. VERO Cell Metabolism in Animal Component-Free Media: Influence of Glucose and Glutamine Substitution
Emma Petiot, Cécile Gény, Fabrice Blanchard, Hervé Pinton, Annie Marc

76. Metabolic Modeling of Drosophila melanogaster Cells Under the Balanced Growth Condition
R.A.M. Piccoli, F.R.X. Batista, A.M. Moraes, M.F. Barral, M.A. Aguiar, P. Léo, E.F.P. Augusto

77. Mass Transfer Considerations for Scale-Up and Scale-Down of Animal Cell Bioprocesses
R. Puskeiler, M. Edler, K. Didzus, R. Müller, J. Gabelsberger

78. Flow Characterization in Wave Bioreactors Using Computational Fluid Dynamics
Alper A. Öncül, Yvonne Genzel, Udo Reichl, Dominique Thévenin

79. Effects of Soy Peptone-Supplemented Medium on CHO-320 Cells
Jean-François Michiels, Jérémie Barbau, Sébastien Sart, Spiros N. Agathos, Yves-Jacques Schneider

80. Very High Protein Production Levels with a New Transient and Serum-Free Expression System Based on Human CAP Cells
Corinna Bialek, Sabine Hertel, Nadine Scholz-Neumann, Ruth Essers, Gudrun Schiedner

81. Recombinant Albumin as an Animal-Free Supplement to Enhance Cell Culture Performance
Collette Sheahan, Marina Ross, Tatyana Mitina, Tom Linke, Tim Robinson, Sally Grosvenor, Larissa Chirkova

82. The SimcellTM High-Throughput Cell Culture System: An Automated Approach to Integrated Cell Line Selection and Process Development
Steve R. C. Warr, Yuen-Ting Chim, A. Peter Russo, Brian Benoit, Mark Uden

83. Advanced In Situ Microscopy for On-Line Monitoring of Animal Cell Culture
P. Wiedemann, F. Egner, H. Wiegemann, J.C. Quintana, W. Storhas, J.S. Guez, C. Schwiebert, H. Suhr

84. Recombinant Antibody Yield Over 2g/L by Transient Transfection of HEK 293 EBNA Cells in a Fed-Batch Process
Divor Kiseljak, Yashas Rajendra, Gaurav Backliwal, David L. Hacker, Lucia Baldi, Florian M. Wurm

85. Rapid Fed-Batch Process Development in SimCell™
Zhihua Xiao, Steve Warr, Yuen-Ting Chim, Donald Lee, Erica Wehling, Ekta Goel, David Zhao, Steve Gorfien

86. Automation of Cell Line Development Using the OptiCHO Expression System
Andrea Salmén, Kristina Lindgren, Lovisa Bylund, Gittan Gelius, Christel Fenge, Ulrica Skoging-Nyberg

87. Modeling of the Evolution of Cellular Density and Nutrient Concentrations in a Fixed-Bed Bioreactor for Its Optimization and Its Scaling-Up
Valérie Gelbgras, Jean-Christophe Drugmand, Benoît Haut

88. Multi-parameter Process Optimization Using the SimCell™ System
A. Peter Russo, Brian Benoit, Christian Wood, David Jan, Sadettin S. Ozturk

89. Online Measurement of pH and O2 Values During Mechanical Stimulation of Cells
Stefanie Böhm, Solvig Diederichs, Thomas Scheper, Martijn Griensven, Cornelia Kasper

90. Reducing Protein Immunogenicity by Design: Deimmunization and Tolerance Induction
Anne S. Groot, Tobias Cohen, Lenny Moise, William D. Martin

91. Second-Generation Interferons: An Effective, Long-Lasting Hyperglycosylated IFN-alpha2
Natalia Ceaglio, Marina Etcheverrigaray, Ricardo Kratje, Harald S. Conradt, Marcos Oggero

92. Large Scale Ex Vivo Manufacture of Blood Cells
Nicholas E. Timmins, Lars K. Nielsen

93. Production of Recombinant Human Leukemia Inhibitory Factor (lif) in a Mammalian Cell Bioreactor: A First Approach
R. P. Baptista, D.M. Barata, L.P. Fonseca, M.M. Diogo, C. Lobato Silva, J.M.S. Cabral

94. Effect of Non-Ionic Surfactant Pluronic F-68 on CHO Cell Kinetics, IFN-γ Production and Glycosylation
M.-F. Clincke, E. Guedon, F.T. Yen, V. Ogier, O. Roitel, B.E. Bihain, J.-L. Goergen

95. Production and Purification of a Human Dll1(ECD)IgGFc Fusion Protein in CHOSFS Cells
Claas Haake, Pierre Moretti, Jeanette Woiterski, Florian P. Limbourg, Cornelia Kasper, Thomas Scheper

96. Controlling Fucosylation Levels of Antibodies with Osmolality During Cell Culture in Several Host Cell Lines
Yoshinobu Konno, Yuki Kobayashi, Ken Takahashi, Shinji Sakae, Masako Wakitani, Toshiyuki Suzawa, Keiichi Yano, Masamichi Koike, Kaori Wakamatu, Shinji Hosoi

97. A High Cell Density Approach to Fed-Batch Cell Culture for Production of Biopharmaceuticals
Sampath Kumar, Kevin McCarthy, Linda Francullo, Kerstin Crowe, Robin Heller-Harrison, Wenge Wang, Gregory Hiller, Mark Leonard

98. Approaches for Humanization of an Anti-idiotypic Murine Monoclonal Antibody
Alexander Mader, Renate Kunert

99. Optimized CGE-LIF-Based Glycan Analysis for High-Throughput Applications
Jana Rödig, René Hennig, Jana Schwarzer, Udo Reichl, Erdmann Rapp

100. Protein Quality Issues for Biopharmaceuticals During Solvent/Detergent Viral Inactivation Steps
Raymond Tyther, Lisa Murphy, Nigel Jenkins

101. Characterisation of Cultivation and Initial Proteome Analysis of the Novel Human Cell Line AGE1.hn
Eva Schräder, Raimund Hoffrogge, Volker Sandig, Thomas Noll

102. Avian Designer Cells AGE1.CR® as Candidates for MVA and Influenza Vaccine Production
Verena Lohr, Alexander Rath, Ingo Jordan, Volker Sandig, Yvonne Genzel, Udo Reichl

103. Constitutive Augmentation of Heat Shock Response in a Producer Cell for Viral Vaccines
Ingo Jordan, Kristin Höwing, Stefanie Oehmke, Volker Sandig

104. In Vitro Approaches for Improved Rotavirus VLP’s Quality
Maria Candida M. Mellado, Manuel J.T. Carrondo, Paula M. Alves

105. Development of Inactivated Polio Vaccine (IPV) Derived from Attenuated Sabin Strains
Wilfried A. M. Bakker, Yvonne E. Thomassen, Aart G. ’t Oever, Janny Westdijk, Monique G.C.T. Oijen, Gideon F.A. Kersten, Leo A. Pol, Rudy Hertroys, Nico Heuvel, Jan Hendriks

106. Development of a Manufacturing Process for the Production of a Canine Adenovirus Type 2 (CAV-2) Vector Using MDCK Cells
Paulo Fernandes, Virgínia Santiago, Núria Viana, Eric J. Kremer, Ana S. Coroadinha, Paula M. Alves

107. Monitoring of Host-Cell Infection and Virus-Induced Apoptosis in Influenza Vaccine Production
Britta Isken, Josef Schulze-Horsel, Andreas Bock, Yvonne Genzel, Udo Reichl

108. Accounting for the Entire Influenza Hemagglutinin during a Flu Vaccine Production Process
T. Kröber, M.W. Wolff, S. Lehmann, A. Zimmermann, Udo Reichl

109. Lentiviral Vectors for Rapid and Efficient Recombinant CHO Cell Line Generation
Agata Oberbek, Mattia Matasci, Myriam Adam, Lucia Baldi, David L. Hacker, Florian M. Wurm

110. Thermal and Detergent Tolerance for a Chimeric Bionanoparticle
Luísa Pedro, Sandra S. Soares, Guilherme N.M. Ferreira

111. A New Approach for Rapid Development of Spodoptera frugiperda/BEVS-Based Processes
Christoph Ries, Virginia Wasem, Dorothea Karrer, Corinne John, Regine Eibl

112. The Role of Culture Medium Lipids and Lipid Metabolism in Retroviral Vector Production Under Serum Deprivation
A.F. Rodrigues, A.I. Amaral, M. Carmo, Paula M. Alves, Ana S. Coroadinha

113. Scale-Down of the Salk Inactivated Polio Vaccine Production Process
Yvonne E. Thomassen, Marian Vinke, Leo A. Pol, Wilfried A.M. Bakker

114. Poxvirus Production on Chicken Embryo Fibroblasts in iCELLis™ Disposable Fixed-Bed Bioreactor
Nicolas Havelange, Martine Marigliano, Martine Sainte-Marie, Fabien Debras, Nadia Tazir, José Castillo

115. Platform Technology for Viral Vaccine Production: Comparison Between Attached and Suspension Vero Cells
Yvonne E. Thomassen, Gerco Eikenhorst, Leo A. Pol, Wilfried A.M. Bakker

116. Workshop 1: New Directions in Pharmaceutical Process Development and Manufacturing: Process Analytical Technology (PAT), Quality by Design (QbD), Design Space (DS), and other FDA Initiatives
Sadettin S. Ozturk, Nigel Jenkins

117. ESACT Workshop: Stem Cell Technology and Tissue Engineering New Developments and Clinical Applications
John R. Masters, Finbarr O’Sullivan

118. The Impact of High-Titer Feedstreams on Monoclonal Antibody Purification
Brian Kelley

119. Workshop on Genomics Technology
Tim Charlebois, Wei-Shou Hu

Keywords: Biomedicine, Biomedicine general, Cell Biology, Life Sciences, general, Biotechnology

Publication year
ESACT Proceedings
Page amount
44 pages
Medicine, Health Care, Mode

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