PCR for Clinical Microbiology

1. PCR Basics
G. James

2. PCR Methodology
Ian Carter, Catriona Halliday, Theo P. Sloots, Todd M. Pryce, Ian D. Kay, Gerald B. Harnett, Glenys R. Chidlow, Philip M. Giffard

3. Laboratory Accreditation Standards
R. Wood, G. Barber

4. Meningitis and Encephalitis
David L. Gordon

5. Respiratory Infections
Michael D. Nissen, Stephen B. Lambert, David M. Whiley, Theo P. Sloots

6. Blood-Borne Disease
Dominic Dwyer, William Rawlinson

7. Enteric Infections
James Buttery

8. Sexually Transmitted Infections
Suzanne M. Garland, David W. Smith, Sepehr N. Tabrizi, David M. Whiley

9.
Bordetella pertussis and Bordetella parapertussis

Leanne Sammels

10. Brucellosis – The Role of PCR in Diagnosis and Management
Shane Byrne, John Bates, Jennifer M. B. Robson

11.
Burkholderia cepacia Complex and Burkholderia gladioli

Timothy M. Kidd

12.
Chlamydia and Lymphogranuloma Venereum (LGV)
Sepehr N. Tabrizi

13. Chlamydophila
James Branley, Bronwen Roy

14. Coxiella burnetii
John Stenos, Stephen Graves, Michelle Lockhart

15. Diarrheagenic Escherichia coli Pathotypes (DEP) Including Enterohaemorrhagic (EHEC)/Shiga-toxin E. coli (STEC)
James Chin, Sam Abraham, Ren Zhang, Rafat Al-Jassim

16. Haemophilus ducreyi and Klebsiella granulomatis
Ian M. Mackay

17.
Haemophilus influenzae – Capsulated and Non-typeable
Gerald B. Harnett, Glenys R. Chidlow

18.
Mycobacterium tuberculosis Complex
Maria Globan, Janet Fyfe

19. Mycobacteria Other Than Mycobacterium tuberculosis

Greg James

20. Mycoplasma genitalium
David M. Whiley

21. Mycoplasma pneumoniae
Rebecca Rockett

22. Neisseria gonorrhoeae
David M. Whiley

23. Neisseria meningitidis
Helen V. Smith

24. Pseudomonas aeruginosa
Snehal Anuj, David M. Whiley

25. Rickettsia
John Stenos, Stephen Graves, Leonard Izzard

26. Streptococcus pneumoniae
Bronwen Roy, Marcel Leroi

27. Treponema pallidum
Gerald B. Harnett, Julia A. Cattell, Tracy M. Perris, Glenys R. Chidlow

28. Universal Bacterial Identification by PCR and DNA Sequencing of 16S rRNA Gene
Greg James

29. Adenovirus
Carl Kirkwood

30. Cytomegalovirus (CMV)
Jonathan Howard

31. Dengue Virus
Greg Smith

32. Enterovirus
Jason A. Roberts, Bruce R. Thorley

33. Epstein Barr Virus
Jonathan Howard

34. Flavivirus
Gerald B. Harnett, Julia A. Cattell

35. Hepatitis A Virus
Scott Bowden

36. Hepatitis B Virus
Scott Bowden

37. Hepatitis C Virus
Scott Bowden

38. Hepatitis D Virus
Scott Bowden

39. Hepatitis E Virus
Scott Bowden

40. Herpes Simplex Virus Type 1 and 2
Neisha Jeoffreys, David M. Whiley

41. Human Bocavirus
Sarah J. Tozer

42. Human Coronaviruses
Rebecca Rockett

43. Human Herpes Viruses -6, -7 and -8
Jonathan Howard

44. Human Papillomavirus
Sepehr N. Tabrizi

45. Human Polyomaviruses – JCV and BKV
Ian Carter, David M. Whiley

46. Human Polyomaviruses – KIV and WUV
Seweryn Bialasiewicz

47. Human Rhinoviruses
Ian M. Mackay

48. Influenza Virus A H5N1 (“Avian Influenza”)
Greg Smith, Ina Smith

49. Influenza Virus A H1N1 (2009) (“Human Swine Influenza”)
David M. Whiley, Theo P. Sloots

50. Influenza Type C
Cassandra Faux

51. Measles Virus
Greg Smith

52. Norovirus
Carl Kirkwood

53. Respiratory Syncytial Virus Types A and B
Gerald B. Harnett, Glenys R. Chidlow

54. Rotavirus
Carl Kirkwood

55. Varicella Zoster virus
Mala Ratnamohan

56. Eight Commonly Recognised Respiratory Viruses
David M. Whiley

57. Detection of CMV, HSV, VZV, EBV and Enterovirus by Multiplex PCR
Christopher McIver, Ian Carter

58.
Aspergillus Species
Catriona Halliday

59. Cryptosporidium
Damien Stark

60. Cyclospora
Damien Stark

61. Dientamoeba fragilis
Damien Stark

62. Entamoeba histolytica
Damien Stark

63. Giardia
Damien Stark

64. Malaria (P. falciparum, P. vivax, P. malariae, P. ovale)
Seweryn Bialasiewicz, David M. Whiley, Theo P. Sloots

65. Microsporidia
Damien Stark

66. Pneumocystis jirovecii
Rebecca Fonte, Ian D. Kay

67. Trichomonas vaginalis
Tuckweng Kok

68.
Toxoplasma gondii

Neisha Jeoffreys

69. Multiplex PCR for Protozoan Parasites
Damien Stark

70. Universal Detection and Identification of Fungi by PCR and DNA Sequencing
Todd M. Pryce

71. An Introduction to Antimicrobial Susceptibility Screening
Graeme R. Nimmo

72. PCR Assays in Detecting Methicillin Resistance in Staphylococci: Coagulase Negative Staphylococci (CNS), S. aureus, and MRSA with the PVL Gene
John Merlino, Ian D. Kay, Geoff Coombs, Silvano Palladino

73. Detection of VRE: vanA and vanB Genes by PCR
John Merlino, Ian D. Kay, Geoff Coombs, Silvano Palladino

74. Metallo β Lactamases Gene blaimp, blaspm and blavim Detection by Multiplex Real-Time TaqMan Assay on the Smartcycler
Ian Carter

75. PCR-Sequencing for Detection of Human Cytomegalovirus Mutations Conferring Antiviral Resistance
Gillian M. Scott

Keywords: Life Sciences, Microbiology, Applied Microbiology, Infectious Diseases, Biological Techniques, Science, general, Medical Microbiology