Analytical Ultracentrifugation

Part I. Introduction

1. Important and Essential Theoretical Aspects of AUC
Susumu Uchiyama, Fumio Arisaka

2. Experimental Design and Practical Aspects
Fumio Arisaka, Susumu Uchiyama

Part II. AUC Instrumentation and Analysis

3. The CFA Analytical Ultracentrifuge Architecture
Thomas M. Laue, J. Brett Austin

4. Fluorescence Detection System
Tao G. Nelson, Glen D. Ramsay, Matthew A. Perugini

5. The Multiwavelength UV/Vis Detector: New Possibilities with an Added Spectral Dimension
Engin Karabudak, Helmut Cölfen

6. SEDANAL: Model-Dependent and Model-Independent Analysis of Sedimentation Data
Peter J. Sherwood, Walter F. Stafford

7. SEDANAL: Global Analysis of General Hetero- and Self-Associating Systems by Sedimentation Equilibrium
Walter F. Stafford, Peter J. Sherwood

8. Analytical Ultracentrifugation Data Analysis with UltraScan-III
Borries Demeler, Gary E. Gorbet

Part III. Hydrodynamic Modeling

9. Introduction: Calculation of Hydrodynamic Parameters
Olwyn Byron

10. Calculation of Hydrodynamic Parameters: US-SOMO
Emre Brookes, Mattia Rocco

11. The HYDRO Software Suite for the Prediction of Solution Properties of Rigid and Flexible Macromolecules and Nanoparticles
José García Torre

12. Accurate Hydrodynamic Modeling with the Boundary Element Method
Sergio R. Aragon

Part IV. Applications of AUC: Material Science

13. Hydrodynamic Analysis of Synthetic Permanently Charged Polyelectrolytes
Christine Wandrey, Hamideh Ahmadloo

14. Different Levels of Self-Sufficiency of the Velocity Sedimentation Method in the Study of Linear Macromolecules
Georges M. Pavlov

Part V. Applications of AUC: Biological Science

15. Applications of Analytical Ultracentrifugation to Membrane Proteins
Karen G. Fleming

16. Protein-Ligand Interactions
Shane E. Gordon, Matthew A. Perugini

17. AUC in the High Concentration of Salts/Cosolvent
Christine Ebel

18. Aspects of the Analytical Ultracentrifuge Determination of the Molar Mass Distribution of Polysaccharides
Stephen E. Harding, Gary G. Adams, Richard B. Gillis, Fahad M. Almutairi, Gordon A. Morris

Part VI. Applications of AUC: Biopharmaceuticals

19. Use of Analytical Ultracentrifugation as an Orthogonal Method for Size Exclusion Chromatography: Assuring Quality for Therapeutic Protein Products and Meeting Regulatory Expectations
John F. Carpenter, David L. Bain, Gibbes R. Johnson

20. Biopharmaceuticals: Application of AUC-SV for Quantitative Analysis of Protein Size Distributions
Amanda A. Cordes, Kelly K. Arthur, John P. Gabrielson

21. Biopharmaceutical Evaluation of Intermolecular Interactions by AUC-SE
Shuntaro Saito, Susumu Uchiyama

Part VII. AUC of High-Concentration Systems and Non-ideal Solutions

22. Johnston-Ogston Effects in Two Simulated Systems of Polystyrene Beads That Are Polydisperse with Respect to Density
Thomas P. Moody

23. Analysis of Nonideal, Interacting, and Noninteracting Systems by Sedimentation Velocity Analytical Ultracentrifugation
Walter F. Stafford

24. Techniques for Dissecting the Johnston-Ogston Effect
John J. Correia, Daniel F. Lyons, Peter Sherwood, Walter F. Stafford

25. Acquisition and Analysis of Data from High Concentration Solutions
Tabot M. D. Besong, Arthur J. Rowe

Part VIII. New Applications of AUC

26. Detection and Quantitative Characterization of Macromolecular Heteroassociations via Composition Gradient Sedimentation Equilibrium
Allen P. Minton

Keywords: Life Sciences, Biochemistry, general, Polymer Sciences, Pharmaceutical Sciences/Technology, Biotechnology, Cell Biology, Analytical Chemistry