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Stanta, Giorgio

Guidelines for Molecular Analysis in Archive Tissues

Stanta, Giorgio - Guidelines for Molecular Analysis in Archive Tissues, ebook

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ISBN: 9783642178900
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Table of contents

1. Archive Tissues
Giorgio Stanta

2. Preanalytical Time Interval (PATI) and Fixation
Lorenzo Daniele, Giuseppe D’Armento, Gianni Bussolati

3. Formalin-Free Fixatives
Isabella Dotti, Serena Bonin, Giorgio Basili, Valentina Faoro

4. Manual Microdissection
Valentina Faoro, Giorgio Stanta

5. Tissue Microarray (TMA)
Valentina Faoro, Anna Sapino

6. Laser Capture Microdissection (LCM)
Elvira Stacher, Hannelore Kothmaier, Iris Halbwedl, Helmut H. Popper

7. DNA Extraction from Formalin-Fixed Paraffin-Embedded (FFPE) Tissues
Serena Bonin, Patricia J. T. A. Groenen, Iris Halbwedl, Helmut H. Popper

8. DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues (FFPE) (from Small Fragments of Tissues or Microdissected Cells)
Serena Bonin, Patricia J. T. A. Groenen, Iris Halbwedl, Helmut H. Popper

9. Fast Protocol for DNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues
Falk Hlubek, Andreas Jung

10. DNA Extraction from Blood and Forensic Samples
Solange Sorçaburu Cigliero, Elisabetta Edalucci, Paolo Fattorini

11. Restoration and Reconstruction of DNA Length
Serena Bonin, Federica Tavano

12. RNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues
Serena Bonin, Giorgio Stanta

13. RNA Extraction from Decalcified and Non-decalcified Formalin-Fixed Paraffin-Embedded Tissues
Marco Alberghini, Stefania Benini, Gabriella Gamberi, Stefania Cocchi, Licciana Zanella

14. RNA Temperature Demodification
Serena Bonin, Giorgio Stanta

15. MicroRNA Extraction from Formalin-Fixed Paraffin-Embedded Tissues
Roberto Cirombella, Andrea Vecchione

16. Quantification of Nucleic Acids
Isabella Dotti, Serena Bonin

17. Integrity Assessment of Nucleic Acids
Isabella Dotti, Serena Bonin

18. DNase Treatment of RNA
Isabella Dotti, Serena Bonin

19. Reverse Transcription
Isabella Dotti, Serena Bonin, Ermanno Nardon

20. General Protocol for End-Point PCR
Serena Bonin, Isabella Dotti

21. Quantitative End-Point PCR
Serena Bonin, Isabella Dotti

22. Nested-PCR
Serena Bonin, Isabella Dotti

23. General Protocol for Dot-Blot
Serena Bonin, Isabella Dotti

24. PCR-ELISA
Christiane Schewe, Wilko Weichert, Manfred Dietel

25. Quantitative Real-Time RT-PCR
Isabella Dotti, Ermanno Nardon, Danae Pracella, Serena Bonin

26. DNA Sequencing from PCR Products
Giorgio Basili, Serena Bonin, Isabella Dotti

27. Pyrosequencing Analysis for Detection of KRAS Mutation
Gerlinde Winter, Gerald Höfler

28. Microsatellite Instability (MSI) Detection in DNA from FFPE Tissues
Damjan Glavač, Ermanno Nardon

29. General Protocol for Loss of Heterozygosity Detection
Damjan Glavač, Ermanno Nardon

30. Qualitative Methylation Status Assessment
Damjan Glavač, Ermanno Nardon

31. Quantitative Methylation Status Assessment in DNA from FFPE Tissues with Bisulfite Modification and Real-Time Quantitative MSP
Ermanno Nardon

32. Comparative Genomic Hybridisation (CGH)
Hannelore Kothmaier, Elvira Stacher, Iris Halbwedl, Helmut H. Popper

33. Multiplex Ligation-dependent Probe Amplification (MLPA)
Ana Pilar Berbegall, Eva Villamón, Samuel Navarro, Rosa Noguera

34. Fluorescence In Situ Hybridization (FISH) on Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Sections
Marta Piqueras, Manish Mani Subramaniam, Samuel Navarro, Nina Gale, Rosa Noguera

35. Introduction on Genome-wide Expression Profiling from Formalin-Fixed Paraffin-Embedded Tissues Using Microarrays
Manfred Dietel, Jan Budczies, Wilko Weichert, Carsten Denkert

36. Introduction
Valentina Faoro, Karl-Friedrich Becker, Giorgio Stanta

37. Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissues
Karl-Friedrich Becker, Christina Schott

38. Rapid Protein Extraction from Formalin-Fixed Paraffin-Embedded Tissue Samples
Valentina Faoro, Giorgio Stanta

39. Protein Extraction Protocol from Tissues Treated with Formalin-Free Fixatives
Valentina Faoro, Giorgio Stanta

40. Trichloroacetic Acid (TCA) Precipitation of Proteins
Valentina Faoro, Giorgio Stanta

41. One-Dimensional Sodium-Dodecyl-Sulfate (SDS) Polyacrylamide Gel Electrophoresis
Valentina Faoro, Karl-Friedrich Becker, Giorgio Stanta

42. Visualization of Proteins
Valentina Faoro, Karl-Friedrich Becker, Giorgio Stanta

43. Western Blotting
Valentina Faoro, Karl-Friedrich Becker, Giorgio Stanta

44. Dot Blot
Valentina Faoro, Giorgio Stanta

45. Membrane Stripping
Valentina Faoro, Giorgio Stanta

46. Reverse-Phase Protein Microarrays
Christina Schott, Karl-Friedrich Becker

47. Introduction
Valentina Faoro, Giorgio Stanta, Alessandro Vindigni

48. Sample Preparation and In Gel Tryptic Digestion for Mass Spectrometry Experiments
Valentina Faoro, Giorgio Stanta

49. MALDI Imaging Mass Spectrometry on Formalin-Fixed Paraffin-Embedded Tissues
Axel Walch, Sandra Rauser, Heinz Höfler

50. Introduction
Giorgio Stanta, Ermanno Nardon, Renzo Barbazza

51. Internal Quality Control (IQC) Organization
Giorgio Stanta, Ermanno Nardon, Renzo Barbazza

52. Standards for Equipment and Reagents
Ermanno Nardon, Renzo Barbazza

Keywords: Medicine & Public Health, Pathology, Molecular Medicine, Oncology

Author(s)
Publisher
Springer
Publication year
2011
Language
en
Edition
1
Category
Medicine, Health Care, Mode
Format
Ebook
eISBN (PDF)
9783642178900

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