Antibody Engineering

Part I. Bioinformatics of Antigen-binding Sites

1. Analysis of Single Chain Antibody Sequences Using the VBASE2 Fab Analysis Tool
Svetlana Mollova, Ida Retter, Michael Hust, Stefan Dübel, Werner Müller

2. Standardized Sequence and Structure Analysis of Antibody Using IMGT®

François Ehrenmann, Patrice Duroux, Véronique Giudicelli, Marie-Paule Lefranc

3. Protein Sequence and Structure Analysis of Antibody Variable Domains
Andrew C. R. Martin

Part II. Generation of Antibody Fragments and Their Derivatives

4. scFv by Two-Step Cloning
Dafne Müller

5. Bivalent Diabodies
Roland E. Kontermann

6. Generation of Single-Chain Fv Fragments and Multivalent Derivatives scFv-Fc and scFv-CH3 (Minibodies)
Tove Olafsen, Vania E. Kenanova, Anna M. Wu

7. Miniantibodies
Jonas V. Schaefer, Peter Lindner, Andreas Plückthun

8. Generation of Stably Transfected Eukaryotic Cell Lines Producing ImmunoRNAse Fusion Proteins
Athanasios Mavratzas, Evelyn Exner, Jürgen Krauss, Michaela A. E. Arndt

9. Antibody–Cytokine Fusion Proteins with Members of the TNF-Family
Dafne Müller, Jeannette Gerspach

10. Recombinant Immunotoxins for Treating Cancer
Ira Pastan, Mitchell Ho

11. T Bodies
Bianca Altvater, Silke Landmeier, Claudia Rossig

12. Expressing Intrabodies in Mammalian Cells
Alessio Cardinale, Silvia Biocca

13. Phenotypic Knockdown with Intrabodies
Nina Strebe, Manuela Schüngel

14. Disulfide-Stabilized Fv Fragments
Ulrich Brinkmann

15. PEGylation of Antibody Fragments to Improve Pharmacodynamics and Pharmacokinetics
Arutselvan Natarajan, Sally J. DeNardo

16. Fusion Proteins with Improved PK
Roland Stork

17.
In Vivo Biotinylated scFv Fragments
Laila Al-Halabi, Torsten Meyer

18. Bispecific Diabodies and Single-Chain Diabodies
Roland E. Kontermann

19. Generation and Characterization of a Dual Variable Domain Immunoglobulin (DVD-Ig™) Molecule
Chengbin Wu, Tariq Ghayur, Jochen Salfeld

20. Isolation of Antigen-Specific Nanobodies
Gholamreza Hassanzadeh Ghassabeh, Dirk Saerens, Serge Muyldermans

21. CDR-FR Peptides
Xiao-Qing Qiu

Part III. Production of Antibody Fragments

22. Purification and Characterization of His-Tagged Antibody Fragments
Martin Schlapschy, Markus Fiedler, Arne Skerra

23. Production of Antibody Fragments in the Gram-Positive Bacterium Bacillus megaterium

Miriam Steinwand, Eva Jordan, Michael Hust

24. Analysis and Purification of Antibody Fragments Using Protein A, Protein G, and Protein L
Remko Griep, John McDougall

25. Purification and Analysis of Strep-tagged Antibody-Fragments
Martin Schlapschy, Arne Skerra

26. Production of Antibodies and Antibody Fragments in Escherichia coli

Dorothea E. Reilly, Daniel G. Yansura

27. Improving Expression of scFv Fragments by Co-expression of Periplasmic Chaperones
Jonas V. Schaefer, Andreas Plückthun

28. Bioreactor Production of scFv Fragments in Pichia pastoris

Stephan Hellwig, Georg Melmer

29. Expression of Antibody Fragments in Transgenic Plants
Udo Conrad, Doreen M Floss

30. Transient Production of scFv-Fc Fusion Proteins in Mammalian Cells
Thomas Schirrmann, Konrad Büssow

Part IV. Recombinant Antibody Molecules in Nanobiotechnology and Proteomics

31. Immunoliposomes
Sylvia K. E. Messerschmidt, Julia Beuttler, Miriam Rothdiener

32. Targeted Polymeric Nanoparticles
Katharina Landfester, Anna Musyanovych

33. Antibody Microarrays for Expression Analysis
Christoph Schröder, Anette Jacob, Sven Rüffer, Kurt Fellenberg, Jörg D. Hoheisel

34. Evaluation of Recombinant Antibodies on Protein Microarrays Applying the Multiple Spotting Technique
Zoltán Konthur, Jeannine Wilde

Part V. Preclinical and Clinical Development

35. Xenograft Mouse Models for Tumour Targeting
Colin Green, Hakim Djeha, Gail Rowlinson-Busza, Christina Kousparou, Agamemnon A. Epenetos

36. Xenograft Mouse Models for Tumour Targeting
Surinder K. Sharma, R Barbara Pedley

37. Imaging Tumor Xenografts Using Radiolabeled Antibodies
Tove Olafsen, Vania E. Kenanova, Anna M. Wu

38. Human Anti-antibody Response
Natalie L. Griffin, Hassan Shahbakhti, Surinder K. Sharma

39. IP Issues in the Therapeutic Antibody Industry
Ulrich Storz

Keywords: Biomedicine, Antibodies, Protein Science, Cell Biology, Biological Techniques, Molecular Medicine