Kontermann, Roland
Antibody Engineering
Part I. Cloning of the Antigen-binding Site from Hybridoma
1. Cloning of Variable Domains from Mouse Hybridoma by PCR
Nina Strebe, Frank Breitling, Dieter Moosmayer, Bodo Brocks, Stefan Dübel
2. Coning Hybridoma cDNA by RACE
Andrew Bradbury
3. Construction of scFv Fragments from Hybridoma or Spleen Cells by PCR Assembly
Jonas V. Schaefer, Annemarie Honegger, Andreas Plückthun
Part II. Generation of Antibody Repertoires
4. Mouse Immune Libraries for the Generation of ScFv Fragments Directed Against Human Cell Surface Antigens
Christian Kellner, Sahar Mohseni Nodehi, Matthias Peipp
5. Human Antibody Gene Libraries
Michael Hust, Stefan Dübel
6. Synthetic Antibody Libraries
Pierre Martineau
7. Immune Libraries from Nonhuman Primates (NHP)
Thibaut Pelat, Michael Hust, Philippe Thullier
8. Generation of Rabbit Immune Libraries
Rüdiger Ridder, Hermann Gram
Part III. Selection of Antibody Fragments from Combinatorial Libraries
9. Immunotube Selections
Roland E. Kontermann
10. Phage Display and Selection in Microtitre Plates
Michael Hust, Michael Mersmann
11. Phage Display and Selections on Biotinylated Antigens
Patrick Chames, Daniel Baty
12. Phage Display and Subtractive Selection on Cells
Steffen U. Eisenhardt, Karlheinz Peter
13. Selection of Phage Antibody Libraries for Binding and Internalization into Mammalian Cells
Yu Zhou, James D. Marks
14. Improving Phage Display Throughput by Using Hyperphage, Miniaturized Titration and pVIII (g8p) ELISA
Frank Breitling, Olaf Broders, Saskia Helmsing, Michael Hust, Stefan Dübel
15. Yeast Display and Selections
Kelly Davis Orcutt, K. Dane Wittrup
16. The Generation of Transgenic Mice Expressing Human Antibody Repertoires
Xiangang Zou, Guocheng Lan, Michael J. Osborn, Marianne Brüggemann
17. Selection of Antibody Fragments by Means of the Filter-Sandwich Colony Screening Assay
Sabine Rauth, Martin Schlapschy, Arne Skerra
18. Semi-automated Magnetic Bead-Based Antibody Selection from Phage Display Libraries
Zoltán Konthur, Jeannine Wilde, Theam Soon Lim
Part IV. Engineering and Production of Immunoglobulins
19. Aspects of Isotype Selection
Zehra Kaymakcalan, Alexander Ibraghimov, Andrew G. Goodearl, Jochen G. Salfeld
20. Generation of Heavy and Light Chains (Chimeric Antibodies)
Kirstin A. Zettlitz
21. Humanising Antibodies by CDR Grafting
David Gareth Williams, David J. Matthews, Tarran Jones
22. Humanization by Resurfacing
Johan Desmet, Karen Vanhoorelbeke, Hans Deckmyn
23. Human Antibodies by Guided Selection
Sang Jick Kim, Insoo Park, Hyo Jeong Hong
24. In Silico De-Immunization
Philippe Alard, Johan Desmet, Ignace Lasters
25. Affinity Maturation by Chain Shuffling and Site Directed Mutagenesis
Jianlong Lou, James D. Marks
26. Affinity Maturation by Random Mutagenesis and Phage Display
Holger Thie
27. Engineering of the Fc Region for Improved PK (FcRn Interaction)
Vania E. Kenanova, Tove Olafsen, Jan T. Andersen, Inger Sandlie, Anna M. Wu
28. Antibody-Dependent Enzyme Prodrug Therapy (ADEPT)
Richard Begent, Surinder Sharma, Kerry Chester
29. Production of Recombinant Human IgG Antibodies in the Baculovirus Expression System
Mifang Liang, Stefan Dübel
30. Expression of IgA Molecules in Mammalian Cells
Thomas Beyer, Stefan Lohse, Michael Dechant, Thomas Valerius
Part V. Antibody Characterization
31. Expression of Complete Antibodies in Transgenic Plants
Doreen M. Floss, Udo Conrad
32. Expression of Full Length Monoclonal Antibodies (mAb) in Algal Chloroplast
Miller Tran, Stephen P. Mayfield
33. Expression of IgG Antibodies in Mammalian Cells
Thomas Jostock, Jiandong Li
34. Protein A/G Chromatography
Kirstin A. Zettlitz
35. Epitope Analysis Using Synthetic Peptide Repertoires Prepared by SPOT Synthesis Technology
U. Beutling, R. Frank
36. Epitope Mapping by Printed Peptide Libraries
Frank Breitling, Christopher Schirwitz, Thomas Felgenhauer, Ines Block, Volker Stadler, Ralf Bischoff
37. Antibody Epitope Mapping Using Yeast Display
Consuelo Garcia-Rodriguez, Yu Zhou, James D. Marks
38. Size Exclusion Chromatography
Bernd Voedisch, Holger Thie
39. Structural Characterization of Antibodies by Mass Spectrometry
Marie-Claire Janin-Bussat, Jean-Marc Strub, Elsa Wagner-Rousset, Olivier Colas, Christine Klinguer-Hamour, Nathalie Corvaia, Alain Dorsselaer, Alain Beck
40. Antibody Glycans Characterization
Marie-Claire Janin-Bussat, Elsa Wagner-Rousset, Christine Klinguer-Hamour, Nathalie Corvaia, Alain Dorsselaer, Alain Beck
41. Affinity Measurements by Competition ELISA
Pierre Martineau
42. Anti-Histidine Antibodies as Tools for Reversible Capturing of His-Tagged Fusion Proteins for Subsequent Binding Analysis
H.-M. Zenn, S. Hutschenreiter, F. W. Herberg
43. Affinity Measurements Using Quartz Crystal Microbalance (QCM)
Thomas Johansson
44. Affinity Measurements with Radiolabeled Antibodies
Verena Boschert, Peter Scheurich
45. Neutralization Tests
Philippe Thullier, Dorothea Thea Sesardic
46. Functional Characterization of Antibodies Neutralizing Soluble Factors
Geertruida M. Veldman, Zehra Kaymakcalan, Renee Miller, Leena Kalghatgi, Jochen G. Salfeld
47. Competitive ELISA
Torsten Meyer
48. Quantification of Human IgG and Related Fc Fusion Proteins by a Human IgG/Fc Capture ELISA
Torsten Rülker, Doris Meier, Thomas Schirrmann
49. Determination of Fc-Mediated Antibody-Effector Functions by Chromium Release Assay
Tina Otz
50. Binding Studies with Flow Cytometry
Thomas Schirrmann
Keywords: Biomedicine, Antibodies, Protein Science, Cell Biology, Biological Techniques, Molecular Medicine
- Author(s)
- Kontermann, Roland
- Dübel, Stefan
- Publisher
- Springer
- Publication year
- 2010
- Language
- en
- Edition
- 1
- Page amount
- 801 pages
- Category
- Medicine, Health Care, Mode
- Format
- Ebook
- eISBN (PDF)
- 9783642011443