Imaging Cellular and Molecular Biological Functions

I. Considerations for Routine Imaging

1. Entering the Portal: Understanding the Digital Image Recorded Through a Microscope
Kristin L. Hazelwood, Scott G. Olenych, John D. Griffin, Judith A. Cathcart, Michael W. Davidson

2. Quantitative Biological Image Analysis
Erik Meijering, Gert van Cappellen

3. The Open Microscopy Environment: A Collaborative Data Modeling and Software Development Project for Biological Image Informatics
Jason R. Swedlow

4. Design and Function of a Light-Microscopy Facility
Kurt I. Anderson, Jeremy Sanderson, Jan Peychl

II. Advanced Methods and Concepts

5. Quantitative Colocalisation Imaging: Concepts, Measurements, and Pitfalls
Martin Oheim, Dongdong Li

6. Quantitative FRET Microscopy of Live Cells
Adam D. Hoppe

7. Fluorescence Photobleaching and Fluorescence Correlation Spectroscopy: Two Complementary Technologies To Study Molecular Dynamics in Living Cells
Malte Wachsmuth, Klaus Weisshart

8. Single Fluorescent Molecule Tracking in Live Cells
Ghislain G. Cabal, Jost Enninga, Musa M. Mhlanga

9. From Live-Cell Microscopy to Molecular Mechanisms: Deciphering the Functions of Kinetochore Proteins
Khuloud Jaqaman, Jonas F. Dorn, Gaudenz Danuser

III. Cutting-Edge Applications and Utilities

10. Towards Imaging the Dynamics of Protein Signalling
Lars Kaestner, Peter Lipp

11. New Technologies for Imaging and Analysis of Individual Microbial Cells
Byron F. Brehm-Stecher

12. Imaging Parasites in Vivo
Freddy Frischknecht, Rogerio Amino, Blandine Franke-Fayard, Chris Janse, Andrew Waters, Robert Ménard

13. Computer-Assisted Systems for Dynamic 3D Reconstruction and Motion Analysis of Living Cells
David R. Soll, Edward Voss, Deborah Wessels, Spencer Kuhl

14. High-Throughput/High-Content Automated Image Acquisition and Analysis
Gabriele Gradl, Chris Hinnah, Achim Kirsch, Jürgen Müller, Dana Nojima, Julian Wölcke

15. Cognition Network Technology – A Novel Multimodal Image Analysis Technique for Automatic Identification and Quantification of Biological Image Contents
Maria Athelogou, Günter Schmidt, Arno Schäpe, Martin Baatz, Gerd Binnig

16. High-Content Phenotypic Cell-Based Assays
Eugenio Fava, Eberhard Krausz, Rico Barsacchi, Ivan Baines, Marino Zerial

Keywords: Life Sciences, Cell Biology, Biochemistry, general, Biological Microscopy, Biological Techniques, Biotechnology