Login

Schepers, Ute

RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C.elegans, Drosophila, and Mammals

Schepers, Ute - RNA Interference in Practice: Principles, Basics, and Methods for Gene Silencing in C.elegans, Drosophila, and Mammals, ebook

133,90€

Ebook, PDF with Adobe DRM
ISBN: 9783527604371
DRM Restrictions

Printing101 pages with an additional page accrued every 8 hours, capped at 101 pages
Copy to clipboard17 excerpts

This hands-on guide to RNA interference brings the power of targeted gene silencing to any laboratory with the basic equipment for handling nucleic acids. In easy-to-follow, step-by-step protocols you will learn:
  • How RNAi works in worms, flies and mammals
  • How to design the most efficient RNAi constructs
  • How to achieve transient, stable and conditional RNAi in cell cultures
  • How to determine the efficiency of an RNAi experiment
  • and how to use RNAi for gene therapy

All the protocols have been thoroughly tested in the author's own laboratory, and she provides examples of successful experiments and troubleshooting hints to help in establishing your own successful RNAi experiments. Also includes a list of suppliers for RNAi reagents and equipment as well as a glossary of terms.

Keywords: SCIENCE / Life Sciences / Cytology SCI017000

Author(s)
Publisher
John Wiley and Sons, Inc.
Publication year
2006
Language
en
Edition
1
Page amount
336 pages
Category
Natural Sciences
Format
Ebook
eISBN (PDF)
9783527604371
Printed ISBN
9783527310203

Similar titles