A Clinician's Guide to Sperm DNA and Chromatin Damage

Part I. Human Sperm Chromatin Structure

1. Sperm Chromatin: An Overview
Rod Balhorn

2. Sperm Nucleoproteins (Histones and Protamines)
Ferran Barrachina, Ada Soler-Ventura, Rafael Oliva, Meritxell Jodar

3. Sperm Nuclear Architecture
W. Steven Ward

Part II. Laboratory Evaluation of Sperm Chromatin and DNA Damage

4. Sperm Chromatin Structure Assay (SCSA®): Evolution from Origin to Clinical Utility
Donald P. Evenson

5. TUNEL Assay
Monica Muratori, Elisabetta Baldi

6. TUNEL Assay by Benchtop Flow Cytometer in Clinical Laboratories
Rakesh Sharma, Zeynep Cakar, Ashok Agarwal

7. The Comet Assay
Elva I. Cortés-Gutiérrez, Martha I. Dávila-Rodríguez, Carmen López-Fernández

8. Sperm Chromatin Dispersion (SCD) Assay
José Luis Fernández, Stephen Johnston, Jaime Gosálvez

9. Cytochemical Tests of Sperm Chromatin Maturity
Juris Erenpreiss, Ksenija Zubkova

10. Is There an Optimal Sperm DNA Test?
Michelle M. Kim, Cigdem Tanrikut

Part III. Etiology of Sperm DNA Damage: Biological and Clinical Factors

11. Oxidative Stress
Ralf Henkel, Michael Solomon

12. Apoptosis in Ejaculated Spermatozoa and in the Normal and Pathological Testes: Abortive Apoptosis and Sperm Chromatin Damage
Denny Sakkas, Hasan M. El-Fakahany

13. Defective DNA Repair in Spermiogenesis
Tiphanie Cavé, Olivier Simard, Marie-Chantal Grégoire, Guylain Boissonneault

14. Defective Spermatogenesis and Sperm DNA Damage
Rakesh Sharma, Ashok Agarwal

15. Sperm Chromatin and Lifestyle Factors
Sarah K. Fatool, Avi Harlev

16. Cancer and Sperm DNA Damage
Peter T. K. Chan, Bernard Robaire

17. Sperm Chromatin and Environmental Factors
Aleksander Giwercman, Marcello Spanó

18. Sperm DNA Damage and Oocyte Repair Capability
Sezgin Gunes, Semra Sertyel

Part IV. Clinical Studies on Utility of Sperm DNA Damage Tests

19. Experimental Studies on Sperm DNA Fragmentation and Reproductive Outcomes
Serafín Perez-Cerezales, Priscila Ramos-Ibeas, Eva Pericuesta Camacho, Raul Fernández-González, Angela Patricia López-Cardona, Ricardo Laguna-Barraza, Noelia Fonseca Balvís, Alejandro Chacón Castro, Benjamin Planells, Alfonso Gutiérrez-Adan

20. Sperm DNA and Natural Pregnancy
Marcello Spanò, Aleksander Giwercman

21. Sperm DNA and ART (IUI, IVF, ICSI) Pregnancy
Mona Bungum, Krzysztof Oleszczuk

22. Sperm DNA and Pregnancy Loss After IVF and ICSI
Nirlipta Swain, Gayatri Mohanty, Luna Samanta

23. Sperm DNA Tests Are Clinically Useful: Pro
Luke Simon, Douglas T. Carrell, Armand Zini

24. Sperm DNA Tests Are Clinically Useful: CON
Katherine Rotker, Mark Sigman

Part V. Treatment Options for Men with Sperm DNA Damage

25. Antioxidant Therapy
Ahmad Majzoub, Ashok Agarwal

26. Varicocelectomy
Matheus Roque, Sandro C. Esteves

27. Physiological Intracytoplasmic Sperm Insemination Based on Hyaluronic Acid-Binding Ability
Mohammad Hossein Nasr-Esfahani, Shaghayegh Foroozan-Boroojeni, Marziyeh Tavalaee

28. Advanced Sperm Processing/Selection Techniques
Ashok Agarwal, Manesh Kumar Panner Selvam

29. Use of Testicular Sperm for ICSI: Pro
Sandro C. Esteves, Matheus Roque

30. Debate on the Use of Testicular Sperm for ICSI: Con
Peter T. K. Chan

31. Strategies to Diminish DNA Damage in Sperm Samples Used for ART
Jaime Gosálvez, Ashok Agarwal, Sandro C. Esteves

32. Sperm DNA Testing: Where Do We Go from Here?
Ahmad H. Al-Malki, Armand Zini

Keywords: Medicine & Public Health, Reproductive Medicine, Andrology, Endocrinology