Multi-Parametric Live Cell Microscopy of 3D Tissue Models

Part I. Introduction: 3D Tissue Models, Methodology and Toolkit

1. Current State-of-the-Art 3D Tissue Models and Their Compatibility with Live Cell Imaging
Katie Bardsley, Anthony J. Deegan, Alicia Haj, Ying Yang

2. Simultaneous Phosphorescence and Fluorescence Lifetime Imaging by Multi-Dimensional TCSPC and Multi-Pulse Excitation
Wolfgang Becker, Vladislav Shcheslavskiy, Angelika Rück

3. Quantitative Live Cell FLIM Imaging in Three Dimensions
Alix Marois, Klaus Suhling

4. Three-Dimensional Tissue Models and Available Probes for Multi-Parametric Live Cell Microscopy: A Brief Overview
Neil O’Donnell, Ruslan I. Dmitriev

Part II. Manufacturing

5. Fabrication and Handling of 3D Scaffolds Based on Polymers and Decellularized Tissues
Anastasia Shpichka, Anastasia Koroleva, Daria Kuznetsova, Ruslan I. Dmitriev, Peter Timashev

Part III. Application Methods and Protocols

6. Multi-Parametric Imaging of Hypoxia and Cell Cycle in Intestinal Organoid Culture
Irina A. Okkelman, Tara Foley, Dmitri B. Papkovsky, Ruslan I. Dmitriev

7. Imaging of Intracellular pH in Tumor Spheroids Using Genetically Encoded Sensor SypHer2
Elena V. Zagaynova, Irina N. Druzhkova, Natalia M. Mishina, Nadezhda I. Ignatova, Varvara V. Dudenkova, Marina V. Shirmanova

8. Application of Fluorescence Lifetime Imaging (FLIM) to Measure Intracellular Environments in a Single Cell
Takakazu Nakabayashi, Kamlesh Awasthi, Nobuhiro Ohta

9. Quantitative Imaging of Ca2+ by 3D–FLIM in Live Tissues
Asylkhan Rakymzhan, Helena Radbruch, Raluca A. Niesner

10. Live Cell Imaging of Viscosity in 3D Tumour Cell Models
Marina V. Shirmanova, Lubov’ E. Shimolina, Maria M. Lukina, Elena V. Zagaynova, Marina K. Kuimova

11. Live Imaging of Cell Invasion Using a Multicellular Spheroid Model and Light-Sheet Microscopy
Marco Marcello, Rosalie Richards, David Mason, Violaine Sée

12. Raman Imaging Microscopy for Quantitative Analysis of Biological Samples
Shinji Kajimoto, Mizuki Takeuchi, Takakazu Nakabayashi

Keywords: Life Sciences, Regenerative Medicine/Tissue Engineering, Biological Microscopy, Biomedical Engineering/Biotechnology, Biomedical Engineering, Spectroscopy and Microscopy