Vega, M. Cristina
Advanced Technologies for Protein Complex Production and Characterization
Part I. Introduction
1. Protein Complex Production from the Drug Discovery Standpoint
Ismail Moarefi
2. Choose a Suitable Expression Host: A Survey of Available Protein Production Platforms
Francisco J. Fernández, M. Cristina Vega
Part II. Prokaryotic Expression Hosts
3. ACEMBL Tool-Kits for High-Throughput Multigene Delivery and Expression in Prokaryotic and Eukaryotic Hosts
Yan Nie, Maxime Chaillet, Christian Becke, Matthias Haffke, Martin Pelosse, Daniel Fitzgerald, Ian Collinson, Christiane Schaffitzel, Imre Berger
4. Complex Reconstitution and Characterization by Combining Co-expression Techniques in
Renaud Vincentelli, Christophe Romier
5. Membrane Protein Production in
Harm Jan (Arjan) Snijder, Jonna Hakulinen
6. Cell-Free Synthesis of Macromolecular Complexes
Mathieu Botte, Aurélien Deniaud, Christiane Schaffitzel
7. A
Rebekka Biedendieck
8. Protein Complex Production in Alternative Prokaryotic Hosts
Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, M. Cristina Vega
Part III. Lower Eukaryotic Expression Hosts
9. Production of Protein Complexes in Non-methylotrophic and Methylotrophic Yeasts
Francisco J. Fernández, Miguel López-Estepa, Javier Querol-García, M. Cristina Vega
10.
Tomoaki Niimi
11. Alternative Eukaryotic Expression Systems for the Production of Proteins and Protein Complexes
Sara Gómez, Miguel López-Estepa, Francisco J. Fernández, Teresa Suárez, M. Cristina Vega
Part IV. Higher Eukaryotic Expression Hosts
12. Fundamentals of Baculovirus Expression and Applications
Thomas A. Kost, Christopher W. Kemp
13. The MultiBac Baculovirus/Insect Cell Expression Vector System for Producing Complex Protein Biologics
Duygu Sari, Kapil Gupta, Deepak Balaji Thimiri Govinda Raj, Alice Aubert, Petra Drncová, Frederic Garzoni, Daniel Fitzgerald, Imre Berger
14. Fundamentals of Expression in Mammalian Cells
Michael R. Dyson
15. Assembling Multi-subunit Complexes Using Mammalian Expression
Bahar Baser, Joop Heuvel
Part V. Plant Expression
16. Microalgae as Solar-Powered Protein Factories
Franziska Hempel, Uwe G. Maier
17. Strategies and Methodologies for the Co-expression of Multiple Proteins in Plants
Albert Ferrer, Monserrat Arró, David Manzano, Teresa Altabella
18. Transient Expression Systems in Plants: Potentialities and Constraints
Tomas Canto
Part VI. Complex Reconstitution
19. Complex Reconstitution from Individual Protein Modules
Jérôme Basquin, Michael Taschner, Esben Lorentzen
20. Structural Reconstruction of Protein-Protein Complexes Involved in Intracellular Signaling
Klára Kirsch, Péter Sok, Attila Reményi
Part VII. Biophysical Methods to Assess Protein Complexes
21. The Use of Small-Angle Scattering for the Characterization of Multi Subunit Complexes
Adam Round
22. Application of Nuclear Magnetic Resonance and Hybrid Methods to Structure Determination of Complex Systems
Filippo Prischi, Annalisa Pastore
Keywords: Life Sciences, Protein Science, Protein Structure, Systems Biology
- Editor
- Vega, M. Cristina
- Publisher
- Springer
- Publication year
- 2016
- Language
- en
- Edition
- 1
- Series
- Advances in Experimental Medicine and Biology
- Page amount
- 13 pages
- Category
- Natural Sciences
- Format
- Ebook
- eISBN (PDF)
- 9783319272160
- Printed ISBN
- 978-3-319-27214-6