Tumor Microenvironment

1. Staining Against Phospho-H2AX (γ-H2AX) as a Marker for DNA Damage and Genomic Instability in Cancer Tissues and Cells
Anika Nagelkerke, Paul N. Span

2. Measuring DNA Replication in Hypoxic Conditions
Iosifina P. Foskolou, Deborah Biasoli, Monica M. Olcina, Ester M. Hammond

3. Isolation of Proteins on Nascent DNA in Hypoxia and Reoxygenation Conditions
Monica M. Olcina, Amato J. Giaccia, Ester M. Hammond

4. In Vivo Interrogation of the Hypoxic Transcriptome of Solid Tumors: Optimizing Hypoxic Probe Labeling with Laser Capture Microdissection for Isolation of High-Quality RNA for Deep Sequencing Analysis
Lauren K. Brady, Vladimir Popov, Constantinos Koumenis

5. Gas Chromatography Coupled to Mass Spectrometry (GC–MS) to Study Metabolism in Cultured Cells
Fiona Grimm, Louise Fets, Dimitrios Anastasiou

6. Probing Cancer Cell Metabolism Using NMR Spectroscopy
Kate E. R. Hollinshead, Debbie S. Williams, Daniel A. Tennant, Christian Ludwig

7. Measuring the Impact of Microenvironmental Conditions on Mitochondrial Dehydrogenase Activity in Cultured Cells
Ramon C. Sun, Albert Koong, Amato Giaccia, Nicholas C. Denko

8. Measuring Autophagy in the Context of Cancer
Michael J. Morgan, Andrew Thorburn

9. Methods for Studying Autophagy Within the Tumor Microenvironment
Sengottuvelan Murugan, Ravi K. Amaravadi

10. Methods: Using Three-Dimensional Culture (Spheroids) as an In Vitro Model of Tumour Hypoxia
Russell Leek, David Robert Grimes, Adrian L. Harris, Alan McIntyre

11. Isolation of Glioma-Initiating Cells for Biological Study
Jing Hu, Geoffrey Markowitz, Xiao-Fan Wang

12. Isolation of Mouse and Human Tumor-Associated Macrophages
Luca Cassetta, Roy Noy, Agnieszka Swierczak, Gaël Sugano, Harriet Smith, Lisa Wiechmann, Jeffrey W. Pollard

13. Mouse Models of Brain Metastasis for Unravelling Tumour Progression
Manuel Sarmiento Soto, Nicola R. Sibson

14. Quantification of Lung Metastases from In Vivo Mouse Models
Joan Chang, Janine T. Erler

15. A Multimodal Data Analysis Approach for Targeted Drug Discovery Involving Topological Data Analysis (TDA)
Muthuraman Alagappan, Dadi Jiang, Nicholas Denko, Albert C. Koong

16. Efficient Protocol for the Identification of Hypoxic Cell Radiosensitisers
Cho Rong Hong, Jingli Wang, Kevin O. Hicks, Michael P. Hay

Keywords: Biomedicine, Cancer Research, Molecular Medicine, Immunology