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Rapley, Ralph

Molecular Biomethods Handbook

Rapley, Ralph - Molecular Biomethods Handbook, ebook

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ISBN: 9781603273756
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Table of contents

A. Nucleic Acid Methods

1. The Manipulation of Nucleic Acids
Gayle Corkill, Ralph Rapley

2. Restriction Enzymes
Gareth J. S. Jenkins

3. Principles and Medical Applications of the Polymerase Chain Reaction
Bimal D. M. Theophilus

4. Probe Design, Production, and Applications
Marilena Aquino de Muro

5. Southern Blotting as a Diagnostic Method
Bronwen M. Harvey, Pirkko Soundy

6. Capillary Electrophoresis of DNA
Beatriz Sanchez-Vega

7. Denaturing High-Performance Liquid Chromatography (DHPLC) for Nucleic Acid Analysis
Kim Hung Leung, Shea Ping Yip

8. Denaturing Gradient Gel Electrophoresis (DGGE)
Jeroen H. Roelfsema, Dorien J. M. Peters

9. Single Strand Conformation Polymorphism (SSCP) Analysis
Kim Hung Leung, Shea Ping Yip

10. Randomly Amplified Polymorphic DNA (RAPD)
Lúcia Maria da Cunha Galvão, Eliane Lages-Silva

11. Quantification of mRNA Using Real Time RT-PCR
David Sugden, Patricia de Winter

12. Quantitative Analysis of DNA Sequences by PCR and Solid-Phase Minisequencing
Anu Suomalainen, Ann-Christine Syvänen

13. Multiplex Amplifiable Probe Hybridization (MAPH)
Carolina Sismani, Ludmila Kousoulidou, Philippos C. Patsalis

14. Gene Expression Profiling
Arnis Druka, Robbie Waugh, Pete Hedley

15. Comparative Genomic Hybridization in Clinical and Medical Research
Peng-Hui Wang, Yann-Jang Chen, Chi-Hung Lin

16. Subtractive Hybridization
Craig Winstanley

17. Fluorescence In Situ Hybridization
Jane Bayani, Jeremy A. Squire

18. Quantitative Trait Locus Mapping to Identify Genes for Complex Traits in Mice
Jonathan D. Smith

19. cDNA Microarrays
Phillip G. Febbo

20. Mapping Techniques
Simon G. Gregory

21. Single Nucleotide Polymorphisms
Nameeta Shah

22. Gene/Protein Sequence Analysis
Bernd H. A. Rehm, Frank Reinecke

B. Protein and Cell Methods

23. Protein Electrophoresis
David Sheehan, Siobhan O'Sullivan

24. Protein Blotting
Patricia Gravel

25. Capillary Electrophoresis of Proteins
Mark Strege

26. Autoradiography and Fluorography
Bronwen M. Harvey

27. Mass Spectrometry of Proteins and Peptides
Kenneth G. Standing

28. Post-Translational Modif ications of Proteins
Christoph Kannicht, Birte Fuchs

29. Protein Microarray Technology
Charlotte H. Clarke, Eric T. Fung

30. Protein–Protein Interactions
Hae Ryoun Park, Lisa Montoya Cockrell, Yuhong Du, Andrea Kasinski, Jonathan Havel, Jing Zhao, Francisca Reyes-Turcu, Keith D. Wilkinson, Haian Fu

31. Glycoprotein Analysis
Terry D. Butters, David C. A. Neville

32. Solid-Phase Peptide Synthesis
Mare Cudic, Gregg B. Fields

33. Monoclonal Antibodies
Zhong J. Zhang, Maher Albitar

34. Antibody Phage Display
Rob Aitken

35. Protein Engineering
Thomas Willemsen, Urs B. Hagemann, Eva M. Jouaux, Sabine C. Stebel, Jody M. Mason, Kristian M. Müller, Katja M. Arndt

36. Directed Protein Evolution
Sabine C. Stebel, Annette Gaida, Katja M. Arndt, Kristian M. Müller

37. Enzyme Linked Immunosorbent Assay (ELISA)
John Crowther

38. Epitope Mapping
Glenn E. Morris

39. Quantum Dots
Charles Z. Hotz

40. Ion-Exchange Chromatography
David Sheehan

41. Size-Exclusion Chromatography
Paul Cutler

42. Hydrophobic Interaction Chromatography
Paul A. O'Farrell

43. Affinity Chromatography
Adam Charlton, Michael Zachariou

44. High Performance Liquid Chromatography (HPLC) of Peptides and Proteins
Tzong-Hsien Lee, Marie-Isabel Aguilar

45. Amino Acid Analysis
Ian Davidson, Paula O'Connor

46. Surface Plasmon Resonance
K. Scott Phillips, Quan Jason Cheng

47. Macromolecular Crystallography
Bernhard Rupp, Katherine A. Kantardjieff

48. Microchip Devices for Bioanalysis
Anna C. Kinsella, Shelley D. Minteer

49. Mammalian Cell Culture
Simon P. Langdon

50. Plant Tissue Culture
Víctor M. Loyola-Vargas, C. De-la-Peña, R. M. Galaz-Ávalos, F. R. Quiroz-Figueroa

51. Stem Cells and Regenerative Medicine
Mohan C. Vemuri, Chellu S. Chetty

52. Cryopreservation
John G. Day, Keith C. Harding, Jayanthi Nadarajan, Erica E. Benson

53. Magnetic Resonance Imaging
Pottumarthi V. Prasad, Pippa Storey

54. Electron Microscopy
John Kuo

55. Confocal Microscopy
Guy Cox

56. Laser Microdissection
Graeme I. Murray

57. Biomedical Uses of Flow Cytometry
James L. Weaver, Maryalice Stetler-Stevenson

58. Immunomicroscopy
Constance Oliver

59. In Situ Hybridization
Ian A. Darby, Tim D. Hewitson

60. High Throughput Screening
William P. Janzen

Keywords: Life Sciences, Biochemistry, general, Molecular Medicine, Human Genetics, Laboratory Medicine, Cell Biology

Author(s)
 
Publisher
Springer
Publication year
2008
Language
en
Edition
1
Page amount
1144 pages
Category
Natural Sciences
Format
Ebook
eISBN (PDF)
9781603273756

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