Applied Biochemistry and Biotecnology

Session 1A. Enzyme Catalysis and Engineering

1. Introduction to Session 1A
Michael E. Himmel

2. Grass Lignocellulose
Danny E. Akin

3. Enzymatic Microreactors for the Determination of Ethanol by an Automatic Sequential Injection Analysis System
Eliana M. Alhadeff, Andrea M. Salgado, Oriol Cos, Nei Pereira, Belkis Valdman, Francisco Valero

4. Optimization of Cyclodextrin Glucanotransferase Production From Bacillus clausii E16 in Submerged Fermentation Using Response Surface Methodology
Heloiza Ferreira Alves-Prado, Daniela Alonso Bocchini, Eleni Gomes, Luis Carlos Baida, Jonas Contiero, Inês Conceição Roberto, Roberto Silva

5. Purification and Characterization of a Cyclomaltodextrin Glucanotransferase From Paenibacillus campinasensis Strain H69-3
Heloiza Ferreira Alves-Prado, Eleni Gomes, Roberto Silva

6. Acetone Powder From Dormant Seeds of Ricinus communis L
Elisa D. C. Cavalcanti, Fábio M. Maciel, Pierre Villeneuve, Regina C. A. Lago, Olga L. T. Machado, Denise M. G. Freire

7. Immobilization of Candida antarctica Lipase B by Covalent Attachment to Green Coconut Fiber
Ana I. S. Brígida, Álvaro D. T. Pinheiro, Andrea L. O. Ferreira, Gustavo A. S. Pinto, Luciana R. B. Gonçalves

8. Pretreatment of Corn Stover by Soaking in Aqueous Ammonia at Moderate Temperatures
Tea Hyun Kim, Y. Y. Lee

9. β-d-Xylosidase From Selenomonas ruminantium of Glycoside Hydrolase Family 43
Douglas B. Jordan, Xin-Liang Li, Christopher A. Dunlap, Terence R. Whitehead, Michael A. Cotta

10. Biodiesel Fuel Production by the Transesterification Reaction of Soybean Oil Using Immobilized Lipase
Otávio L. Bernardes, Juliana V. Bevilaqua, Márcia C. M. R. Leal, Denise M. G. Freire, Marta A. P. Langone

11. Thermoinactivation Mechanism of Glucose Isomerase
Leng Hong Lim, Bradley A. Saville

12. Measuring Cellulase Activity
Tor Soren Nordmark, Alan Bakalinsky, Michael H. Penner

13. Enzymatic Hydrolysis Optimization to Ethanol Production by Simultaneous Saccharification and Fermentation
Mariana Peñuela Vásquez, Juliana Nascimento C. Silva, Maurício Bezerra Souza, Nei Pereira

14. Filter Paper Degrading Ability of a Trichoderma Strain With Multinucleate Conidia
Hideo Toyama, Makiko Yano, Takeshi Hotta, Nobuo Toyama

15. Use of Glucose Oxidase in a Membrane Reactor for Gluconic Acid Production
Luiz Carlos Martins Neves, Michele Vitolo

16. Enzyme Production by Industrially Relevant Fungi Cultured on Coproduct From Corn Dry Grind Ethanol Plants
Eduardo A. Ximenes, Bruce S. Dien, Michael R. Ladisch, Nathan Mosier, Michael A. Cotta, Xin-Liang Li

17. Production of Biosurfactant by Pseudomonas aeruginosa Grown on Cashew Apple Juice
Maria V. P. Rocha, Maria C. M. Souza, Sofia C. L. Benedicto, Márcio S. Bezerra, Gorete R. Macedo, Gustavo A. Saavedra Pinto, Luciana R. B. Gonçalves

18.
Thermoascus aurantiacus CBHI/Cel7A Production in Trichoderma reesei on Alternative Carbon Sources
Zsuzsa Benkő, Eszter Drahos, Zsolt Szengyel, Terhi Puranen, Jari Vehmaanperä, Kati Réczey

Session 1B. Plant Biotechnology and Genomics

19. Introduction to Session 1B
Mariam B. Sticklen

20. Heterologous Acidothermus cellulolyticus 1,4-β-Endoglucanase E1 Produced Within the Corn Biomass Converts Corn Stover Into Glucose
Callista Ransom, Venkatesh Balan, Gadab Biswas, Bruce Dale, Elaine Crockett, Mariam Sticklen

21. The Impact of Enzyme Characteristics on Corn Stover Fiber Degradation and Acid Production During Ensiled Storage
Haiyu Ren, Tom L. Richard, Kenneth J. Moore

22. Fractionation of Cynara cardunculus (Cardoon) Biomass by Dilute-Acid Pretreatment
Mercedes Ballesteros, M. José Negro, Paloma Manzanares, Ignacio Ballesteros, Felicia Sáez, J. Miguel Oliva

23. Heat Extraction of Corn Fiber Hemicellulose
Zsuzsa Benkő, Alexandra Andersson, Zsolt Szengyel, Melinda Gáspár, Kati Réczey, Henrik Stålbrand

24. An Evaluation of British Columbian Beetle-Killed Hybrid Spruce for Bioethanol Production
Alex Berlin, Claudio Muñoz, Neil Gilkes, Sepideh Massoumi Alamouti, Pablo Chung, Kyu-Young Kang, Vera Maximenko, Jaime Baeza, Juanita Freer, Regis Mendonça, Jack Saddler

25. Production of Cellulolytic and Hemicellulolytic Enzymes From Aureobasidium pulluans on Solid State Fermentation
Rodrigo Simões Ribeiro Leite, Daniela Alonso Bocchini, Eduardo Silva Martins, Dênis Silva, Eleni Gomes, Roberto Silva

26. The Effect of Particle Size on Hydrolysis Reaction Rates and Rheological Properties in Cellulosic Slurries
Rajesh K. Dasari, R. Eric Berson

27. Effect of Dissolved Carbon Dioxide on Accumulation of Organic Acids in Liquid Hot Water Pretreated Biomass Hydrolyzates
G. Peter Walsum, Maurilio Garcia-Gil, Shou-Feng Chen, Kevin Chambliss

28. Separation of Glucose and Pentose Sugars by Selective Enzyme Hydrolysis of AFEX-Treated Corn Fiber
Robert J. Hanchar, Farzaneh Teymouri, Chandra D. Nielson, Darold McCalla, Mark D. Stowers

29. The Potential in Bioethanol Production From Waste Fiber Sludges in Pulp Mill-Based Biorefineries
Anders Sjöde, Björn Alriksson, Leif J. Jönsson, Nils-Olof Nilvebrant

30. Dilute Sulfuric Acid Pretreatment of Agricultural and Agro-Industrial Residues for Ethanol Production
Carlos Martin, Björn Alriksson, Anders Sjöde, Nils-Olof Nilvebrant, Leif J. Jönsson

31. Xylanase Contribution to the Efficiency of Cellulose Enzymatic Hydrolysis of Barley Straw
María P. García-Aparicio, Mercedes Ballesteros, Paloma Manzanares, Ignacio Ballesteros, Alberto González, M. José Negro

32. Effect of Organosolv Ethanol Pretreatment Variables on Physical Characteristics of Hybrid Poplar Substrates
Xuejun Pan, Dan Xie, Kyu-Young Kang, Seung-Lak Yoon, Jack N. Saddler

33. Liquid Hot Water Pretreatment of Olive Tree Pruning Residues
Cristóbal Cara, Inmaculada Romero, Jose Miguel Oliva, Felicia Sáez, Eulogio Castro

34. Ammonia Fiber Expansion Pretreatment and Enzymatic Hydrolysis on Two Different Growth Stages of Reed Canarygrass
Tamika C. Bradshaw, Hasan Alizadeh, Farzaneh Teymouri, Venkatesh Balan, Bruce E. Dale

35. Mitigation of Cellulose Recalcitrance to Enzymatic Hydrolysis by Ionic Liquid Pretreatment
Anantharam P. Dadi, Constance A. Schall, Sasidhar Varanasi

36. Evaluation of Different Biomass Materials as Feedstock for Fermentable Sugar Production
Yi Zheng, Zhongli Pan, Ruihong Zhang, John M. Labavitch, Donghai Wang, Sarah A. Teter, Bryan M. Jenkins

37. Techno-Economic Analysis of Biocatalytic Processes for Production of Alkene Epoxides
Abhijeet P. Borole, Brian H. Davison

38. Evaluation of Liquid-Liquid Extraction Process for Separating Acrylic Acid Produced From Renewable Sugars
M. E. T. Alvarez, E. B. Moraes, A. B. Machado, R. Maciel Filho, M. R. Wolf-Maciel

39. Enhancement of Rhamnolipid Production in Residual Soybean Oil by an Isolated Strain of Pseudomonas aeruginosa

C. J. B. Lima, F. P. França, E. F. C. Sérvulo, M. M. Resende, V. L. Cardoso

40. Optimizing Carbon/Nitrogen Ratio for Biosurfactant Production by a Bacillus subtilis Strain
R. R. Fonseca, A. J. R. Silva, F. P. Franca, V. L. Cardoso, E. F. C. Sérvulo

41. A New Process for Acrylic Acid Synthesis by Fermentative Process
B. H. Lunelli, E. R. Duarte, E. C. Vasco Toledo, M. R. Wolf Maciel, R. Maciel Filho

42. Ethanol/Water Pulps From Sugar Cane Straw and Their Biobleaching With Xylanase from Bacillus pumilus

Regina Y. Moriya, Adilson R. Gonçalves, Marta C. T. Duarte

43. Nisin Production Utilizing Skimmed Milk Aiming to Reduce Process Cost
Angela Faustino Jozala, Maura Sayuri Andrade, Luciana Juncioni Arauz, Adalberto Pessoa, Thereza Christina Vessoni Penna

44. Bacterial Cellulose Production by Gluconacetobacter sp. RKY5 in a Rotary Biofilm Contactor
Yong-Jun Kim, Jin-Nam Kim, Young-Jung Wee, Don-Hee Park, Hwa-Won Ryu

45. Biosurfactant Production by Cultivation of Bacillus atrophaeus ATCC 9372 in Semidefined Glucose/Casein-Based Media
Luiz Carlos Martins Neves, Kátia Silva Oliveira, Márcio Junji Kobayashi, Thereza Christina Vessoni Penna, Attilio Converti

46. Evaluation of the pH- and Thermal Stability of the Recombinant Green Fluorescent Protein (GFP) in the Presence of Sodium Chloride
Marina Ishii, Juliana Sayuri Kunimura, Hélio Tallon Jeng, Thereza Christina Vessoni Penna, Olivia Cholewa

47. Carboxymethylcellulose Obtained by Ethanol/Water Organosolv Process Under Acid Conditions
Denise S. Ruzene, Adilson R. Gonçalves, José A. Teixeira, Maria T. Pessoa de Amorim

48. Response Surface Methodological Approach for Optimization of Free Fatty Acid Removal in Feedstock
Gwi-Taek Jeong, Do-Heyoung Kim, Don-Hee Park

49. Optimization of Lipase-Catalyzed Synthesis of Sorbitan Acrylate Using Response Surface Methodology
Jeong Gwi-Taek, Park Don-Hee

50. Renewable Energy in the United States
Alvin O. Converse

51. Optimizing the Logistics of Anaerobic Digestion of Manure
Emad Ghafoori, Peter C. Flynn

52. The Relative Cost of Biomass Energy Transport
Erin Searcy, Peter Flynn, Emad Ghafoori, Amit Kumar

53. The Effect of Initial Cell Concentration on Xylose Fermentation by Pichia stipitis

Frank K. Agbogbo, Guillermo Coward-Kelly, Mads Torry-Smith, Kevin Wenger, Thomas W. Jeffries

54. Construction and Evaluation of a Clostridium thermocellum ATCC 27405 Whole-Genome Oligonucleotide Microarray
Steven D. Brown, Babu Raman, Catherine K. McKeown, Shubha P. Kale, Zhili He, Jonathan R. Mielenz

55. Tannase Production by Solid State Fermentation of Cashew Apple Bagasse
Tigressa H. S. Podrigues, Maria Alcilene A. Dantas, Gustavo A. S. Pinto, Luciana R. B. Gonçalves

56. Studying Pellet Formation of a Filamentous Fungus Rhizopus oryzae to Enhance Organic Acid Production
Wei Liao, Yan Liu, Shulin Chen

57. Comparison of Multiple Gene Assembly Methods for Metabolic Engineering
Chenfeng Lu, Karen Mansoorabadi, Thomas Jeffries

58. Fed-Batch Production of Glucose 6-Phosphate Dehydrogenase Using Recombinant Saccharomyces cerevisiae

Luiz Carlos Martins Neves, Adalberto Pessoa, Michele Vitolo

59. Conversion of Aqueous Ammonia-Treated Corn Stover to Lactic Acid by Simultaneous Saccharification and Cofermentation
Yongming Zhu, Y. Y. Lee, Richard T. Elander

Session 5. Bioprocessing and Separations R&D

60. Introduction to Session 5
Luca Zullo, Seth W. Snyder

61. Economic Evaluation of Isolation of Hemicelluloses From Process Streams From Thermomechanical Pulping of Spruce
Tobias Persson, Anna-Karin Nordin, Guido Zacchi, Ann-Sofi Jönsson

62. Estimation of Temperature Dependent Parameters of a Batch Alcoholic Fermentation Process
Rafael Ramos Andrade, Elmer Ccopa Rivera, Aline C. Costa, Daniel I. P. Atala, Francisco Maugeri Filho, Rubens Maciel Filho

63. Thermophilic Anaerobic Digester Performance Under Different Feed-Loading Frequency
John Bombardiere, Teodoro Espinosa-Solares, Max Domaschko, Mark Chatfield

64. A Proposed Mechanism for Detergent-Assisted Foam Fractionation of Lysozyme and Cellulase Restored With β-Cyclodextrin
Vorakan Burapatana, Elizabeth A. Booth, Ian M. Snyder, Ales Prokop, Robert D. Tanner

65. Study on the Production of Biodiesel by Magnetic Cell Biocatalyst Based on Lipase-Producing Bacillus subtilis

Ming Ying, Guanyi Chen

66. Production of ω-3 Polyunsaturated Fatty Acids From Cull Potato Using an Algae Culture Process
Zhanyou Chi, Bo Hu, Yan Liu, Craig Frear, Zhiyou Wen, Shulin Chen

67. Hybrid Neural Network Model of an Industrial Ethanol Fermentation Process Considering the Effect of Temperature
Ivana C. C. Mantovanelli, Elmer Ccopa Rivera, Aline C. Costa, Rubens Maciel Filho

68. Characterization of Thermostructural Damages Observed in a Seaweed Used for Biosorption of Cadmium
Antonio Carlos Augusto Costa, Aderval S. Luna, Robson Pafumé

69. Ethanol Fermentation of Various Pretreated and Hydrolyzed Substrates at Low Initial pH
Zsófia Kádár, San Feng Maltha, Zsolt Szengyel, Kati Réczey, Wim Laat

70. The Effects of Engineering Design on Heterogeneous Biocatalysis in Microchannels
Frank Jones, Robert Bailey, Stephanie Wilson, James Hiestand

71. Utilization of Condensed Distillers Solubles as Nutrient Supplement for Production of Nisin and Lactic Acid from Whey
Chuanbin Liu, Bo Hu, Shulin Chen, Richard W. Glass

72. Optimization of Tocopherol Concentration Process From Soybean Oil Deodorized Distillate Using Response Surface Methodology
Vanessa Mayumi Ito, César Benedito Batistella, Maria Regina Wolf Maciel, Rubens Maciel Filho

73. Semicontinuous Production of Lactic Acid From Cheese Whey Using Integrated Membrane Reactor
Yebo Li, Abolghasem Shahbazi, Sekou Coulibaly, Michele M. Mims

74. Functional Stability of a Mixed Microbial Consortium Producing PHA From Waste Carbon Sources
Erik R. Coats, Frank J. Loge, William A. Smith, David N. Thompson, Michael P. Wolcott

75. Separating a Mixture of Egg Yolk and Egg White Using Foam Fractionation
Tiffany M. Ward, Ross A. Edwards, Robert D. Tanner

76. Synthesis of Poly(Sorbitan Methacrylate) Hydrogel by Free-Radical Polymerization
Gwi-Taek Jeong, Kyoung-Min Lee, Hee-Seung Yang, Seok-Hwan Park, Jae-Hee Park, Changshin Sunwoo, Hwa-Won Ryu, Doman Kim, Woo-Tae Lee, Hae-Sung Kim, Wol-Seog Cha, Don-Hee Park

77. Hybrid Thermochemical/Biological Processing
Robert C. Brown

78. Physical and Chemical Properties of Bio-Oils From Microwave Pyrolysis of Corn Stover
Fei Yu, Shaobo Deng, Paul Chen, Yuhuan Liu, Yiqin Wan, Andrew Olson, David Kittelson, Roger Ruan

79. Comparison of Diafiltration and Size-Exclusion Chromatography to Recover Hemicelluloses From Process Water From Thermomechanical Pulping of Spruce
Alexandra Andersson, Tobias Persson, Guido Zacchi, Henrik Stålbrand, Ann-Sofi Jönsson

Keywords: Chemistry, Biotechnology, Biochemical Engineering