Gendelman, Howard E.

Current Laboratory Methods in Neuroscience Research

Gendelman, Howard E. - Current Laboratory Methods in Neuroscience Research, ebook


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Table of contents

Part I. Neurohistological Techniques

1. Brain Tissue Preparation, Sectioning, and Staining
Jingdong Zhang, Huangui Xiong

2. Brain Stereotaxic Injection
Jingdong Zhang, Huangui Xiong

3. Neuronal Tract Tracing with Light and Electron Microscopy
Jingdong Zhang, Huangui Xiong

4. Stereological Analysis
Kristi M. Anderson, Adam M. Szlachetka, R. Lee Mosley

Part II. In Vitro Preparations

5. Preparation and Use of Rodent Hippocampal Slices
Huangui Xiong, Jianxun Xia

6. Single-Cell Neuronal Dissociation for Electrophysiological Studies
Yu-Long Li

7. Isolation and Culture of Human Neurons, Microglia, and Astrocytes
Li Wu, Santhi Gorantla

8. Isolation and Culture of Neural Stem/Progenitor Cells
Hui Peng, Qiang Chen, Jialin Zheng

9. Isolation of Mitochondria from Brain Tissue and Cells
Changhai Tian, Jialin Zheng

10. Isolation of Synaptosomes from Archived Brain Tissues
Gurudutt Pendyala, James L. Buescher, Howard S. Fox

Xenopus Oocytes Isolation and Microinjection
Huangui Xiong

Part III. Leukocyte Isolation and Application in Neuroscience

12. Centrifugal Elutriation for Studies of Neuroimmunity
Santhi Gorantla, Myhanh Che, Howard E. Gendelman

13. Flow Cytometry in Neuroscience Research
Charles Kuszynski

Part IV. Standard Laboratory Nucleic Acid and Protein Detections

14. Western Blotting Technique in Biomedical Research
Jianuo Liu, James Haorah, Huangui Xiong

15. Polymerase Chain Reaction (PCR) and Real-Time PCR
Georgette D. Kanmogne

Part V. Nanomedicine

16. Nanoformulations
Xin-Ming Liu, Tatiana K. Bronich

17. Neuronanomedicine
JoEllyn M. McMillan, Xin-Ming Liu, Howard E. Gendelman

Part VI. Bioimaging

18. Confocal Imaging of Nerve Cells
You Zhou

19. Magnetic Resonance Imaging
Yutong Liu

20. X-Ray, Positron Emission, and Single Photon Emission Tomographic Bioimaging
Katherine A. Estes, Jacob C. Peterson, Adam M. Szlachetka, R. Lee Mosley

21. Noninvasive Neurophysiological Imaging with Magnetoencephalography
Tony W. Wilson

22. Multiphoton Brain Imaging
Anna Dunaevsky

Part VII. Neuroelectrophysiology

23. Techniques for Extracellular Recordings
Jingdong Zhang, Jianxun Xia, Huangui Xiong

24. “Blind” Patch-Clamp Recordings from Rodent Hippocampal Slices
Jianxun Xia, Huangui Xiong

25. Whole-Cell Patch-Clamp Recording
Matthew J. Hook, Wallace B. Thoreson

Part VIII. Immunohistochemistry and Autoradiography

26. Analysis of Receptor Binding and Quantitative Autoradiography
L. Charles Murrin

27. Techniques in Immunohistochemistry and Immunocytochemistry
Yang Yuan, Jyothi Arikkath

Part IX. Analysis of Gene Expression

28. Protein Identification by Mass Spectrometry: Proteomics
Melinda Wojtkiewicz, Kelley Barnett, Pawel Ciborowski

29. DNA Microarrays as a Tool for Neurosciences Research
James D. Eudy, Lynette Smith

30. Metabolomics
Yazen Alnouti

31. Bioinformatic Methods and Resources for Neuroscience Research
Chittibabu Guda

Part X. Animal Models: Behavior and Pathology

32. Motor Function in Rodent Models of Neurodegenerative Disorders
Jessica A. Hutter Saunders, Max V. Kuenstling, Robert A. Weir, R. Lee Mosley, Howard E. Gendelman

33. Humanized Mice
Larisa Y. Poluektova, Edward Makarov

34. Animal Models for PD and ALS
Max V. Kuenstling, Adam M. Szlachetka, R. Lee Mosley

35. Animal Models of Alzheimer’s Disease
Tomomi Kiyota

36. Animal Models: Behavior and Pathology: Preclinical Assessment of the Putative Cognitive Deficits in HAND
Landhing M. Moran, Rosemarie M. Booze, Charles F. Mactutus

Keywords: Biomedicine, Neurosciences, Neurology, Neurobiology

Publication year
Page amount
598 pages
Medicine, Health Care, Mode
Printed ISBN

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