Principles and Technical Aspects of PCR Amplification

1. The Polymerase Chain Reaction

2. A Brief Comparison Between In Vivo DNA Replication and In Vitro PCR Amplification

3. The PCR in Practice

4. The Different Types and Varieties of Nucleic Acid Target Molecules

5. PCR Primers

6. Deoxynucleotide Triphosphates and Buffer Components

7. Taq and Other Thermostable DNA Polymerases

8. Important Considerations for Typical, Quantitative and Real-Time PCR Protocols

9. Analysis of PCR Amplification Products

10. Ensuring PCR Quality – Laboratory Organisation, PCR Optimization and Controls

11. Ensuring PCR Quality – Quality Criteria and Quality Assurance

12. Variants and Adaptations of the Standard PCR Protocol

13. In Situ PCR Amplification (ISA) – Major Considerations, Sample Processing and Applications

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Keywords: SCIENCE / Life Sciences / Biology / General SCI008000