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Ham, Bryan M.

Proteomics of Biological Systems: Protein Phosphorylation Using Mass Spectrometry Techniques

Ham, Bryan M. - Proteomics of Biological Systems: Protein Phosphorylation Using Mass Spectrometry Techniques, ebook

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ISBN: 9781118137031
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Phosphorylation is the addition of a phosphate (PO4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the use of bioinformatic Internet tools such as the Blast2GO gene ontology (GO) tool, used to help understand and interpret complex data collected in these studies.

Keywords: Mass Spectrometry, phosphorylation, phosphate (PO4) group, mass spectrometry and phosphorylation, phosphoproteomic, phosphoproteomic sample preparation, Blast2GO gene ontology, protein phosphorylation using MS, protein phosphorylation using mass spectrometry, proteomics

Author(s)
Publisher
John Wiley and Sons, Inc.
Publication year
2012
Language
en
Edition
1
Page amount
320 pages
Category
Natural Sciences
Format
Ebook
eISBN (ePUB)
9781118137031
Printed ISBN
9781118028964

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