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Wu, Ge

Assay Development: Fundamentals and Practices

Wu, Ge - Assay Development: Fundamentals and Practices, ebook

157,95€

Ebook, PDF with Adobe DRM
ISBN: 9780470583111
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Essential principles and practice of assay development

The first comprehensive, integrated treatment of the subject, Assay Development: Fundamentals and Practices covers the essentials and techniques involved in carrying out an assay project in either a biotechnology/drug discovery setting or a platform setting.

Rather than attempting comprehensive coverage of all assay development technologies, the book introduces the most widely used assay development technologies and illustrates the art of assay development through a few commonly encountered biological targets in assay development (e.g., proteases, kinases, ion channels, and G protein-coupled receptors). Just enough biological background for these biological targets is provided so that the reader can follow the logics of assay development. Chapters discuss:

  • The basics of assay development, including foundational concepts and applications

  • Commonly used instrumental methods for both biochemical assays and cell-based assays

  • Assay strategies for protein binding and enzymatic activity

  • Cell-based assays

  • High-throughput screening

An in-depth study of the now popular Caliper's off-chip kinase assay provides an instructive, real-world example of the assay development process.

Keywords: Wu, Ge Wu, assay development, assays for purified protein, cell-based assays, high-throughput screening, optimal assays, endogenous mediators, drug discovery, biomarker development, preclinical testing, preclinical testing, Cell & Molecular Biology, Analytical Chemistry, Cell & Molecular Biology, Analytical Chemistry

Author(s)
Publisher
John Wiley and Sons, Inc.
Publication year
2010
Language
en
Edition
1
Page amount
448 pages
Format
Ebook
eISBN (PDF)
9780470583111
Printed ISBN
9780470191156

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