Fussenegger, Martin
Animal Cell Technology Meets Genomics
Chapter 1. Cellular Mechanisms
1. Effect of Osmotic Pressure on Gs-Ns0 Expression System
Mon-Han Wu, George Dimopoulos, Athanasios Mantalaris, Julie Varley
2. Molecular Approaches to Influence Epigenetic Effectors of Transient and Stable Transgene Expression in Mammalian Cells
D. L. Hacker, M. Cotten, F. M. Wurm
3. Genomic Exploration on Chinese Hamster Ovary Cells
M. Leon Gatti, K. Wlaschin, A. Rink, A. Sanny, K.S. Tan, P.M. Nissom, P.F. Ong, K. Wong, R.J. Philip, B. Cham, C.F. Wong, K.M. Lim, M. Yap, W.-S. HU
4. Roles for Glycosylation in Receptor-Ligand Interactions in the Immune System
Pauline M. Rudd, Anthony H. Merry, Raymond A. Dwek
5. Investigations on Mannose-6-Phosphate Receptor Mediated Protein Uptake
M. Dürrschmid, C. Jursik, N. Borth, R. Grabherr, O. Doblhoff-Dier
6. Analysis of Multiple Apoptosis Parameters Using a Microfluidic Chip-Based System
M. Valer, T. Preckel, G. Luedke, C. Buhlmann
7. Inhibiting Apoptosis in Cell Culture Using Multiple Inhibitors
Bruno Figueroa, Eric Ailor, Mitchell Reff, J. Marie Hardwick, Michael Betenbaugh
8. Pausing of Mammalian Cells by Cold Exposure, Limits and Opportunities
L. Hunt, D. Hacker, H. Abridi, F. Grosjean, M. Dejesus, M. Jordan, F. M. Wurm
9. The Functional Competence of Animal Cells: Anal Ysis of the Secretory Pathway
Daniel E. Alete, Andrew J. Racher, John R. Birch, David C. James, C. Mark Smales
10. Proteome Analysis of Recombinant CHO Cells Under Hyperosmotic Stress
Moon Sue Lee, Kyoung Wook Kim, Young Hwan Kim, Gyun Min Lee
11. Expression Profiling Analysis of Sodium Butyrate-Induced Chinese Hamster Ovary Cells in Defined Medium
Mark W. Melville, Martin Sinacore, Louane E. Hann
12. Intracellular Nucleotide Pools for Optimizing Product-Oriented Transient Transfection of Hek293 Cells in Suspension
M.M. Bassani Molinas, C. Beer, F. Hesse, M. Wirth, Durocher, A. Kamen, R. Wagner
13. Impact of Yeast Pyruvate Carboxylase on the Productivity of Animal Host Cell Lines
M. Bollatifogolin, N. Irani, A.J. Beccaria, C. Schulz, J. Heuvel, C.B. Elias, E. Carpentier, Y. Durocher, L. Bisson, M. Etcheverrigaray, R. Kratje, M. Wirth, A. Kamen, R. Wagner
14. Environmental Effects of Lactate on High-Five™ Insect Cell Metabolism
J.-C. Drugmand, Y.-J. Schneider, S.N. Agathos
15. Correlation of Intracellular Nucleotide Pools to Amino Acid Concentrations in Culture Media by the Application of Multivariate Methods
F. Hesse, A. Nelving, R. Wagner
16. Engineering
M.-C. Locas, C. B. Elias, S. Lanthier, L. Bisson, M. Perrier, A.A. Kamen
17. Insect Cell Culture Medium Selection and Optimisation Based on Monitoring and Economical Considerations
M. Lecina, A. Soley, P. Passamani, A. Casablancas, J. Gràcia, C. Vela, E. Espuña, J.J. Cairó, F. Gòdia
18. Antiapoptotic Activity of Synthetic and Nnatural Peptides
František Franek
19. Effect of Antiapoptotic Genes Expression on Cell Growth and Monoclonal Antibody Productivity in a Hybridoma Cell Line
S. Juanola, J. Vives, J. Andersen, E. Prats, L. Cornudella, J.J. Cairó, F. Gòdia
20. Amplified Dicistronic Expression Units Mediate Apoptosis Protection in CHO-DG44 Cells Adapted for Growth in Serum-Free Media, Impact on Mitochondria Copy Number
H. Meents, B. Enenkel, M. Fussenegger
21. Effect of Reduced Water on the Apoptotic Cell Death Triggered by Oxidative Stress in Pancreatic ß HIT-T15 Cell
Y. P. Li, K. Teruya, Y. Katakura, S. Kabayama, K. Otsubo, S. Morisawa, Y. Ishii, Z. Gadek, S. Shirahata
22. Characterization of Apoptosis in a CHO Cell Line Cultivated in Batch and Continuous Culture: Effect of Medium, Specific Perfusion Rate and Chemical Inhibitors
John Thrift, Glen Yamasaki, Rudiger Heidemann, Nada Abbas, Konstantin Konstantinov
23. Hydrogen Peroxide-Induced Cellular Senescence is Regulated via Two Different Pathways
K. Yoshizaki, Y. I. Katakura, T. Fujiki, T. Tsunematu, K. Teruya, S. Shirahata
24. Identification of Autocrine Factors Influencing Proliferation in Serum-Free Cultures of
U. Eriksson, L. Häggström
25. The Effects of Insulin and Long™R
C.A. Yandell, I.P. Butler, A.J. Sheehan, B.J. Wade, A.P. Simula, C. Goddard
26. Conditioned Medium Factors in Protein-Free Cultures of NS0 Cells
E. Spens, L. Häggström
27. Production of Human Anti-Peptide Antibody for Clinical Use by
T. Tamura, Y. Katakura, M. Yamashita, Y. Aiba, S. Matsumoto, K. Teruya, S. Shirahata
28. Optimization of in Vitro Immunization Protocol to Produce Antigen Specific Human Monoclonal Antibody by Demonstrating the Role of IL-10
Q.H. Xu, Y. Katakura, M. Yamashita, K. Teruya, S.G. Fang, S. Shirahata
29. Eextracellular Histone H4 from SF9 Cells Is Antimicrobial
K. Calles, E. Lindskog, Å. Nossed, I. Svensson, L. Häggström
30. Analyzing Calcium Phosphate Transfection of Adherent CHO Cells Using Microscopic Live Imaging
F. Grosjean, M. Jordan, F. M. Wurm
31. Effects of Multiplicity of Infection (MOI) and Cell Cycle on Baculovirus Infection Kinetics
R. Haas, S. Reid, L.K. Nielsen
32. Non-Freezing Preservation and Subsequent Recovery of Hepatocytes Under PO
Kai IDing, Heino Büntemeyer, Jürgen Lehmann
33. Selection of MDCK Subclones with Various Genotypic and Phenotypic Markers: Sensitivity to Influenza Virus Infection
Nicole Kessler, Ghislaine Roche, Audrey Vernet, Alexandra Erny, Christophe Abismil
34. Extracellular Metalloproteases and Their Inhibitors Expressed by The IFN-? Producing CHO-320 Cell Line
J. Mols, F. Verhoeye, C. Peeters-Joris, S.N. Agathos, Y.-J. Schneider
35. Novel Small-Molecule AKT1 Inhibitors Discovered by Redistribution™-Based High- Throughput Screening
Morten Præstegaard, Søren Jensby Nielsen, Betina Kerstin Lundholt, Ivan Mikkelsen, Steven Loechel, Morten Heide, Viggo Linde
36. Influence of Mistletoe Extracts and Its Components on in Vitro Physiology of Cancer Cells
F. Sidler, T. Wermelinger, L. Rist, A. Hensel, A. Viviani
37. Rapid in-Process Monitoring of Antibody Integrity, Purity and Titre- 2100 Bioanalyser (Lab on a Chip) Study
Nesredin A. Mussa, Kee Y. Cheung, Imtiaz M. Alam, Steve Flatman
38. Toxicological Evaluation of Haine River Waste-Water Samples to Appropriately Sensitized Cultured Fathead Minnow Cells Compared With the Microtox® Method
P.J. Dierickx, C. Wielen
Chapter 2. Cell Based Therapies
39. 3D-Cultivation and Characterisation of Osteogenic Cells for the Production of Highly Viable Bone Tissue Implants
M. Barthold, I. Majore, S. Fargali, F. Stahl, R. Schulz, S. Lose, H. Mayer, V. Jäger
40. Carbon Source Has a Considerable Influence on Extracellular Matrix Formation, Demonstrated on Specific Collagen Expression of Primary Human Tenocytes
D. Möbest, M. Jaeger, G. Hoben, D. Hamann, S. Wollner, G.B. Stark
41. Design and Characterization of Cardiomyocyte-Derived Microtissues
Jens M. Kelm, Elisabeth Ehler, Jean-Claude Perriard, Martin Fussenegger
42. Process Development for Standardized Generation of Monocyte-Derived Dendritic Cells: Applicability in Breast Cancer Immunotherapy
H. R. Bohnenkamp, T. Noll
43. Stemline™ Hematopoietic Stem Cell Expansion Medium, a Serum-Free Medium for the Expansion of CD34
S.L. Leugers, D.W. Allison, B.J. Pronold, G. Rennebeck, G. Zant, J.D. Tario, F.J. Swartzwelder, L.M. Donahue
44. Mesenchymal Stem Cells: A Very First Step Towards Large Scale Cell Culture Strategies
M. Bensellam, C. Bassens, M. Toallati, S. Lowagie, J. Werenne
45. Expansion of Murine Embryonic Stem Cells on Microcarriers-Functional Characteristics and Scale-Up Potential
M. Burg, G. Quel, M. Ruediger, R. Zweigerdt
46. In Vitro Cultivation of Rabbit Mesenchymal Stromal Cells on 3D Bioresorbable Calcium Phosphate Scaffolds for the Generation of Bone Tissue Implants
S. Fargali, M. Barthold, M. Rohde, I. Majore, V. Jäger
47. Establishment and Characterization of Human Medullary Thyroid Carcinoma Cell Lines for Immunotherapy
G. Stadler, R. Voglauer, M. Wieser, H. Katinger, R. Pfragner
48. Suppression of Cell Growth by Platinum Nanocolloids as Scavengers against Reactive Oxygen Species
T. Hamasaki, T. Kashiwagi, S. Aramaki, T. Imada, T. Komatsu, Y. Li, K. Teruya, Y. Katakura, S. Kabayama, K. Otsubo, S. Morisawa, S. Shirahata
49. Comparison of Fluidized Bed and Fed Batch Reactor Cultures for Production of Anti-HIV-Antibody
P. Hinterleitner, F. Unterluggauer, K. Landauer, D. Müller, R. Kunert, G. Blüml, O. Doblhoff-Dier, H. Katinger
50. Suppression of Oxidative Stress-Induced Apoptosis of Neuronal Cells by Electrolyzed-Reduced Water
T. Kashiwagi, T. Hamasaki, S. Kabayama, M. Takaki, K. Teruya, Y. Katakura, K. Otubo, S. Morisawa, S. Shirahata
51. Spheroid Formation by Encapsulation of Cancer Cells to Mimic Small Size Tumors
A. Marc, E. Markvicheva, C. Jourdain, L. Bezdetnaya, J.L. Merlin, F. Guillemin, V. Zubov, J.L. Goergen
52. Bioreactor for Continuous Biomechanical Characterization of Cellular Systems and Tissue-Engineered Biohybrid Tissues
D. Möbest, M. Jaeger, J. Guttmann, M. Schneider, G.B. Stark
53. Membrane-Separated Cocultivation of Cord Blood Hematopoietic Stem Cells with Stromal Cell Lines
T. Fischbach, T. Noll
54. Anti-Apoptotic Genes Protect an Insulinoma Cell Line From Cell Death Induced by Hypergl Ycemia
Akiko Ogawa, Satoshi Terada, Masao Miki, Toshihisa Kimura, Akio Yamaguchi
55. Preparation and Characterization of an Idiotype-Dendritic Cell Vaccing for Immunotherapy of Multiple Myeloma
B. Opalka, P. Schütt, D. Brandhorst, U. Buttkereit, M. Tewes, T. Moritz, S. Seeber, M.R. Nowrousian
56. Pretreatment with Polycations Enhances Adenoviral Infection Levels in Serum-Free PER.C6? Cells as Well as Hek 293 Cells
J. Padilla, V. Burke, J. Johnson, J. Hartshorn, S. Mcnorton, K. Etchberger
57. Optimizing the Production of Cardiomyocytes from Mouse Embryonic Stem Cells in a 2L Stirred Tank Reactor
M. SCHROEDER, S. Niebruegge, A. Werner, R. Zweigerdt, M. Burg, J. Lehmann
58. Establishment and Characterization of CD4+ Killer-T-Cells (KTCS) a Promising New Tool for Adoptive Immunotherapy
R. Voglauer, C. Jursik, M. Prchal, H. Jungfer, J. Grillari, H. Katinger
Chapter 3. Gene Based Therapeutics
59. Successful Development of a Robust Adenovirus Production Process Primarily Relies on a Better Understanding of Packaging Cell Line Physiology and Vector Replication Kinetics
A. Kamen, O. Henry, D. Jacob, A. Bernier
60. Retroviral Vector Stability: Inactivation Kinetics and Membrane Properties
P.E. Cruz, M. Carmo, A.S. Coroadinha, A. Bengala, D. Gonçalves, M. Teixeira, O.-W. Merten, C. Geny-Fiamma, M.J.T. Carrondo
61. Comparison of Host Cell Lines and Production Methods for a New Generation of Oncolytic Adenoviral Vectors
Sean Forestell, Chris Celeri, Chris Dang, Tom Gong, Matthew Olsen, Inesse Sifi, Inn Yuk, Scott Geyer
62. Process Development for a Veterinary Vaccine against Heartwater
I. Marcelino, M. Sousa, C. Veríssimo, C. Peixoto, M.J.T. Carrondo, P.M. Alves
63. A New Method for the Quantitative Determination of Enveloped Viral Particles
C. Beer, M. Wirth
64. Development of a 293T Cell Line for the Production of Virus Like Particles in Bioreactors
Maria João L. Costa, Rui Malhó, João gonçalves, Guilherme N.M. Ferreia, MAria Raquel Aires-Barros
65. Effect of MOI and Medium Composition on Adenovirus Infection Kinetics
Tiago B. Ferreira, Paula M. Alves, Délia Gonçalves, M.J.T. Carrondo
66. Ion-Exchange Chromatography Based Strategies for Purification of Viral Vectors
J. Vasi, R. Morenweiser, K. Eriksson, R. Lemmens, S. Herzer
67. Scaling up of Endothelial Cells Culture for the Production of a Rickettsiae Vaccine: Valuation of the Importance of the Interference Effects
V. Hendrick, N. Kagye, Y. Kamba, S. Simon, A-L. THINSY, T. Marique, J. Werenne
Chapter 4. Target Discovery
68. Inducible Gene Expression for Antibiotic Drug Discovery and Diagnostics
Wilfried Weber, Nils Link, Manuela Spielmann, Bettina Keller, Cornelia C. Weber, Martin Fussenegger
69. Nucleic Acid Capture Assay: A Novel High- Throuhgput Method for Direct Mrna Quantitation
J. Goor, P. Tsai., A. Wong, S. Mehta, L. Zheng, L.O. Elliott, J.P. Stephan
70. High Throughput Transient Transfections of Hek293Ebna Cells — Devel Opment of an Integrated Expression Purification Process at 100 ml Scale
Holger Heine, Laurence Rey, Christian Y. Arod, Jean-Philippe Gaudry, Bruno Antonsson, Georg Feger, Thierry Battle
71. Transient Gene Expression in Suspension Hek293 Cells: Application to Large-Scale Protein Production
L. Baldi, R. Jacquet, S. Picasso, P. Tromba, E. Derow, P. Girard, D. Hacker, F. M. Wurm
72. Monoclonal Antibody Production: Determination of Appropriate System and Medium for Production Enhancement and Decrease of Overall Production Time
K. Fritchman, C. Tilsaghani, T. Sumnall
73. The Secrets of Transfection in Serumfree Suspension Culture
S. Geisse, N. Maiuta, B. Buren, M. Henke
74. From Genes to Proteins: Full Open Reading Frame (ORF) Clones and Their Application in Functional Research
A. Goedde, S. Baars, K. Czerny, E. Schultz, N. Gernold, T. Scheuermann, R. Schatten, S. Henze, M. Schick, L. Ebert, C. Langlais, B. Korn
75. A Versatile Disposable Culture System for High Throughput Screening of Process Parameters and Production Cell Lines
M. Jordan, M. J. Jesus, C. Eigenmann, T. Gleich, F. M. Wurm
76. Long Time Storage of Pre-Inoculated Multi-Well PLlates for Cell-Based Assay Kits Used for Drug Iedntification and Characterisation
Alexander Loa, Claudia Gelli, Bernd Laffert
77. Comparative Secretion Levels of Recombinant Proteins between Insect Cell-Bbaculovirus and Mammalian Hek293EBNA Cells
Christophe Losberger, Thierry Bbattle
78. Microfluidic Technology Applied to Protein Sizing and Quantitation
M. Valer, T. Neumann, P. Barthmaier, M. Kuschel, C. Buhlman
79. Cryostock a Software for Cell Culture Management
Marion Zerlin, Lars Greiffenberg, Yannick Wurm, Irvin Winkler, Dieter Kreusel, Stefan Toemoe, Laszlo Ballabas, Jens Martin
Chapter 5. Biopharmaceuticals
80. Targeting the Human Immunoglobulin Loci for High Level Hgene
K. Winkler, T. Wermelinger, C. Paul, S Koch, R. Brecht, S. Zietze, R. Nuck, G. Thiel, U. Marx, V. Sandig
81. Mar Elements as Tools to Increase Protein Production by CHO Cells
P.-A. Girod, M. M. Zahn-Zabal, N. Mermod
82. Cell Line Engineering for Production of Therapeutic Antibody Glycoforms with Increased Biological Activity
Pablo Umana, Peter Bruenker, Claudia Ferrara, Samuel Moser, Tobias Suter, Christian Gerdes, Ursula Puentener, Joel Jean-Mairet, Patrick Buholzer, Manuel Spaeni
83. Development of a Metabolically Optimized Fermentation Process Based on Glucose-Limited CHO Perfusion Culture
Link T, Essers R, Zörner K, Gätgens J, Graaf A, Backstrom M, Hansson G, Noll T
84. Applications of Quasi Real-Time Metabolic Flux Analysis in Mammalian Cell Culture Process Development
Chetan Goudar, Richard Biener, James Michaels, Chun ZHang, James Piret, Konstantin Konstantinov
85. Data-Based Modelling of Cell Cultures
C. Cunha-Bakeev, J. Glassey, G. Montague, M. -Rubeal, P. Hardwicke
86. Cell Culture and Downstream Processing Integration Using Proteinchip® Technology Application to Protein Analysis and Purification in Biopharmaceutical Manufacturing
S. Bengio, P. Santambien, S. Cleverley
87. The Prospects for Insect Cells for Use as a Cell Substrate for the Production of Biotherapeutics
D.N. Galbraith
88. High Performance Cell Culture Platform Technology for MAb Production
David Y.H. Chang, Paul Garza, Yao-Ming Huang, Mylene Talabardon, Soheil Rahmati, Eric Fallon, Wolfgang Noe
89. Development and Integration of a New Animal-Component-Free Process for the Production of UK-279,276
S. Pluschkell, L. Blocker, R. Geldart, S. Hawrylik, P. Mensah, C. Okediadi, S. Pias, A. Subashi, M. Zhu
90. Towards an Affinity-Based Capture System for the Isolation of High Expression Cells Using a Co-Expressed Surface Protein
M. Derouazi, H.M. Pick, C. Deluz, S. Picasso, R. Jacquet, F.M. Wurm
91. Effect of Doxycycline-Regulated Calnexin and Calreticulin Expression on Specific Thrombopoietin Productivity of Recombinant CHO Cells
Joo Young Chung, Seung Wook Lim, Yeon Joo Hong, Yeo Wook Koh, Sun Ok Hwang, Gyun Min Lee
92. Recombinant Glycoprotein Expression Development of a Homologous CHO Expression System
C.B. Noone, T.J. Smith, M.T. Cairns
93. Generation of Recombinant Antibody Manufacturing Cell Lines Using Cell-Cell Fusion
Wenlin Zeng, Ela Puchacz, Katy Heineken, Isaac Raymond, Conway Chang, Thomas Ryll, Steve Chamow
94. Cloning and Expression of Biopharmaceuticals in CHO.K1 and BHK-21 Cells
G.F. Asensi, J.M. Cavalcanti, P.M. Sousa, E.M. Aguila, J.T. Silva, V.M.F. Paschoalin, L.R. Castilho
95. Camel Single-Domain Antibodies as Modular Building Blocks to Make Bivalent Constructs for Use in Immunotherapy of Cancer
Virna Cortez-Retamozo, N. Backmann, P. Senter, U. Wernery, P. Baetselier, S. Muyldermans, H. Revets
96. Establishment of Efficient Cloning Method for Variable Region Genes of Antigen Specific Human Monoclonal Antibody
S. Matsumoto, Y. Katakura, M. Yamashita, Y. Aiba, E. Noguchi, K. Teruya, S. Shirahata
97. From Gene to Monoclonal Antibody: Efficient Screening by Cell Sorting
Borth N., E. Böhm, J. Grillari, M. Löscher, S. Gross, R. Voglauer, B. Ferko, R. Kunert, H. Katinger
98. Development of an Integrated Strategy for Recombinant Cell Line Selection
A. J. Castillo, S. Víctores, E. Faife, Y. Rabasa, K. R. Laluz
99. Gene Transfer to a Animal Cells in Culture by Microinjection: A Novel Tool to Create Recombinant Cell Lines?
M. Derouazi, P. Girard, M. Jordan, C. Denoya, F.M. Wurm
100. Evaluation of the Stability of NS0 Cell Lines Expressing Recombinant Human IgG
Wyn Forrest-Owen, Lekan Daramola, Diane Hatton, Ray Field
101. High Throughput Screening and Selection of Single Cells in Suspension Using Fluorescence Parameters
C. Giese, C.D. Demmler, G. Gradl, U. Marx
102. Control of Key Parameters in the Development of Mammalian Production Clones
R. Kunert, S. Wolbank, M. Chang, S. Preis, W. Steinfellner, N. Borth, H. Katinger
103. Establishment of Screening Systems for Recombinant Cell Lines: Early Prediction of the Suitability as a Production Clone
Ch. Lattenmayer, R. Kunert, H. Katinger
104. Characterisation of CHO Subclones Showing Profound Changes in Performance When Propagated in Different Cultivation Systems
E. Trummer, D. Müller, W. Steinfellner, R. Kunert, F. Steindl, F. Hesse, H. Katinger
105. High Level Production of Recombinant IgG in the Human Cell Line PER.C6™
C. Yallop, M. Raamsman, M. Zuijderwijk, Y. Noordenburg, A. Vooys, R. Keehnen, B. Montfort, M. Jansen, F. Lagerwerf, R. Dijkstra, M. Birrento, M. Vocht, S. Renger, A. Bout, D.-J. Opstelten
106. Creating a New Medium to Help Meet the Variable Nutritional Requirements of Chinese Hamster Ovary (CHO) Cell Clones
Z. Deeds, A. Albee, B. Delong, J. Gifford, S. Ross, K. Kao, M. Caple
107. Development of an Efficient Medium Optimization Kit for Factorial Matrix Design—A Statistical Approach to Increase Cell Growth and Productivity of Recombinany CHO Cells
B. Delong, A. Albee, Z. Deeds, J. Gifford, S. Ross, K. Kao, M. Caple
108. An Efficient Approach to Cell Culture Medium Optimization — A Statistical Method to Medium Mixing
J. Gifford, A. Albee, ZW. Deeds, B. Delong, K. Kao, S. Ross, MV. Caple
109. Virus Inactivation in Foetal Calf Serum by a Combined Treatment of Gammairradiation and UV-C Irradiation
H. Hermann, R. Burian, R. Waldmann
110. Advances in Media Optimization: Two Automated Approaches that Increase Expression While Reducing Development Time
Stacy Holdread, Cindy Hunt, Perry Haaland, Bryce Chaney, Warren Porter, Mohammad Heidaran, Jon Wannlund
111. The Importance of Cholesterol for Insect Cell Growth and Baculovirus Production
Linda H.L. Lua, Steven Reid
112. Use of Rapeseed Proteins and Peptides as Supplements in Insect Cell Cultures
A. Marc, V. Deparis, C. Durrieu, I. Marc, J.L. Goergen, I. Chevalot
113. Nutritional Performance of Wheat-Gluten-Derived Protein Hydrolysates in Hybridoma Cell Cultures. A Nutritional Function Study
Dirk E. Martens, Hendrikus B.A. Wegkamp, Nicholas H. Simpson, Hans H. Huttinga, Ton Kunst, André D. Siemensma
114. Synthechol™ Ynthetic Cholesterol for Cholesterol Dependent Cell Culture — Development of Non-Animal Derived Chemically Defined NS0 Medium
D. Talley, B. Cutak, E. Rathbone, T. -Kolla, D. Allison, J. Blasberg, K. Kao, M. Caple
115. NS0 Derivatives: MAB Production in Largescale SFM Formats
J. Manwaring, B. Barnett, B. Pence, W. Whitford
116. Silk Protein Sericin Accelerates Proliferation of Various Mammalian Cells
Satoshi Terada, Kana Yanagihara, Kozue Kaito, Masao Miki, Masahiro Sasaki, Kazuhisa Tsujimoto, Hideyuki Yamada
117. Image Analysis Based Real Time-Control of Glucose Concentration
T. Brueckerhoff, J.-G. Frerichs, K. Joeris, K. Konstantinov, T. Scheper
118. Online Analysis of Microcarrier Cultivations
Arne Burzlaff, Cornelia Kasper, Thomas Scheper
119. Error Analysis during Estimation of Metabolic Fluxes through Metabolite Balancing
Chetan Goudar, Richard Biener, James Michaels, James Piret, Konstantin Konstantinov
120. Generalized Logistic Equation Modeling of Mammalian Cell Batch Cultures
Chetan Goudar, Rüdiger Heidemann, Klaus Joeris, James Michaels, James Piret, Konstantin Konstantinov
121. In-Situ Microscopy Based Monitoring of Mammalian Cell Culture Processes
Klaus Joeris, Jan-Gerd Frerichs, Thomas Scheper, Konstantin Konstantinov
122. A Noel Disposable Microtube for Rapid Assessment of Biomass in Cell Cultures
Martin Jordan
123. Intracellular Monitoring of Baculovirus Infection of SF9 Insect Cells and R-Protein Production Using Anti-Alpha-1,3/4 Fucosyl Transferase Antibodies and Flow Cytometry
A. Marc, V. Deparis, A. Jeston, J.-L. Goergen
124. Effect of Glucose and Glutamine Concentration on Metabolism of Animal Cells in Chemostat Culture
H. Matsuoka, T. Takeda
125. Evaluation of a Novel apacitance Probe for On-Line Monitoring of Viable Cell Densities in Batch and Fed-Batch Animal Cell Culture Processes
G. Schmid, D. Zacher
126. Sodium Butyrate Enhancement of Protein Expression in Hela Cells: Follow-Up of a Chimeric Reporter Gene Behaviour for Process Development
C. Bassens, P. Rigaux, V. Hendrick, M. Cherlet, K. Sato, K. Kotarsky, J. Werenne
127. Scalable, Serum-Free, High Efficiency Transfection of CHO Cells Using an Economical DNA Transfer Vehicle
P. Girard, M. Derouazi, F. Tilborgh, F.M. Wurm
128. Process Optimization for an NS0-Derived Hybridoma Cell Line in a Chemically Defined, Protein-Free Medium
J. M. Johnson, J. Hartshorn, S. Mcnorton, J. A. Padilla, T. Mohabbat, S. Luo, K. Etchberger
129. Impact of Change in Fermentation Process pH and Dissolved Carbon Dioxide Concentration on Secreted Antibody Structure and Cell Physiology of a GS-NS0 Cell Line Expressing a Human Antibody
Alison Ridley, Jonathan Dempsey, Chris Gee, Richard Turner, Matthew Osborne, Steve Ruddock, Christy Ritchie, Ray Field
130. Experimental Factorial Design for the Study of Some Variables in Roller Bottles Cell Culture
E.N. Rodríguez, M. Pérez, Y. Ordaz, P.R. Casanova, L. Martinez, N. Herrera
131. Evaluation of Rollercell System for High Scale Production of Biopharmaceuticals
E.N. Rodríguez, M. Pérez, Y. Ordaz, P.R. Casanova, L. Martinez, N. Herrera
132. Strategies for the Process Development of Monoclonal Antonpdoes for Therapeutic Use
C. Mattei, N. Dt Bernardi, F. Gaspari, S. Castiglioni, A. Orlandi, E. Muru, L. Cavenaghi, M.L. Nolli
133. Optimisation of PEI-Mediated Transient Expression in Chinese Hamster Ovary Cells
Andrew S. Tait, Mike Hoare, John Birch, Douglas J. Galbraith, MIchael Hines, Catherine J. Brown, David C. James
134. Development of Fed-Batch Process Producing Monoclonal Antibodies Using In-House Media
Yao-Ming Huang, Thu Huynh, Le Ly, Wolfgang Noe, David Y. H. Chang
135. Production of an Anti ß2 Integrin Monoclonal Antibody by a Hybridoma Cell Line Grown in a 2 Liter Bioreactor
H. Kallel, A. Bellila, D. M. Fathallah
136. Economic Evaluation of Disposables vs Stainless Steel within a Modular Pilot Plant Facility — a Comparative Analysis
Miriam Monge, Andrew Sinclair
137. CGMP Manufacturing of a Fusion Protein in Mammalian Cells Using a Large-Scale Acoustic Perfusion System
John Crowley, Carina Schlukebir, Maikel Dijkstal, Suzan Hoeksma, Edith Olthof, Stefanie Hußmann, Sybille Esser, Andreas Herrmann, Jose M. Coco Martin, Robert Hof
138. Influence of Culture Parameters on Viability in Perfusion Process
Mcf Dalm, Smr Cuijten, Wmj Grunsven, A Oudshoorn, J Tramper, De Martens
139. Use of Hydrocyclone as an Efficient Tool for Cell Retention in Perfusion Cultures
E.A. Elsayed, G.-W. Piehl, J. Nothnagel, R.A. Medronho, W.-D. Deckwer, R. Wagner
140. Effect of Different Variables on the Long-Term Spinfilter Clogging during Pilot-Scale Animal Cell Perfusion Runs
A Figueredo, J. Navarrete, P. Vitón, E. Martínez, A. Castro, E. Chico
141. Integrating Acoustic Perfusion in Mammalian Cell Culture: Temperature Control
T. Keijzer, F. Trampler, A. Oudshoorn, H. V/D Bberg
142. Flexible Fed-Batch Process Control of Animal Cells
B. Frahm, R. Pörtner
143. Development of a Fed-Batch Strategy for the Production of NS0-Derived Humanised Mab in Protein Free Medium
E. Ojito Magaz, M.A Arias, E. Chico Veliz
144. Transfection to Manufacturing: Reducing Timelines for High Yielding GS-CHO Processes
M. Rendall, A. Maxwell, D. Tatham, P. Khan, R.D. Gay, R.C. Kallmeier, J.R.T. Wayte, A.J. Racher
145. Optimization of a Fed-Batch Process Producing Humanized Antibodies
Paul Sauer
146. Optimized Feeding Strategy for NS0 Cells
Jennifer Walowitz, Lia Tescione, William Paul, David Jayme, Stephen Gorfien
147. Comparison of Complementary Interactions between Synthetic Ligands and t-PA by Hpmdc
Alexander Tappe, Gerlinde Kretzmer, Cornelia Kasper, Thomas Scheper, Evgenia G. Vlakh, Tatiana B. Tennikova
148. Effect of Nutrient Supplementation on the Biological Quality of tPA Produced by CHO Cells on Serum-Free Medium
C. Altamirano, A. ILlanes, R. Canessa, S. Becerra, J. Berrios
149. Modulation of the Glycosylation Repertoire of a Recombinant Human EPO Expressing Model Cell Line Under Different Culture Conditions
S. Duvar, J. Berlin, H. Ziehr, H.S. Conradt
150. Physicochemical Estimation of rhEPO Potency Produced in Suspension Culture
M. Etcheverrigaray, G. I. Amadeo, C. Didier, D. A. Pereira Bacci, F. A. Cavatorta, R. Kratje
151. Post-Translational Modification of Recombinant Human GM-CSF Expressed by Stable-Transfected CHO Cells under Different Culture Conditions
A.G. Forno, M. Etcheverrigaray, M. Nimtz, R.B. Kratje
152. Softmix, a new Scalable Mixing System for the Cultivation of Sensitive Cells on Microcarrier under Protein- Free Conditions
S. Naschberger, K. Rütten, D. Müller, H. Katinger
153. Establishment of Fermentation Process for Hrepo Using Stirred Tank Fermenters in Perfusion Mode Using A Commercial Protein-Free Culture Medium
L. Bouzó, E. Ojito, M.A. Arias, J. Suarez, E. Chico, A. ALvarez, M. Chea, II. Alvarez, C. Díaz, O. Valiente
154. Continuous Gmp Production in Hollow Fibre Bioreactors — Aviable Alternative for the Production of Low Volume Biopharmaceuticals
R. Brecht, H. Bushnaq-Josting, S. Zietze, R. Nuck, C. Kloth, A. Schwadtke, N. Obermayer, U. Marx, S. Koch
155. Optimizing Cultivation Strategies in Different Scales of Hollow Fiber Bioreactors
G. Rodriguez, M.A. Arias, J. Suárez, M. Chea, L. Bouzó, R. Cuervo, I. Alvarez, E. Chico
156. Increasing Antibody Production from Hollow Fiber Systems
Michael J. Gramer
157. Production of Poliovirus, Rubella Virus, Measles Virus and Recombinant Adenovirus in Protein-Free Media
Cory J. Card, Thomas Smith, Brent Hunsaker, Bill Barnett
158. Serum-Free Production of Poliovirus: A Comparative Study Using Microcarriers, Roller Bottles and Stationary Cell Culture
Cory J. Card, Thomas Smith, Brent Hunsaker, Bill Barnett
159. Glutamine-Free Media for Vaccine Production Processes
Y. Genzel, R. Alt, U. Reichl
160. Serum Free Cultivation of Primary Chicken Embryo Fibroblasts in Microcarrier Systems for Vaccine Production
B. Hundt, A. Schänzler, U. Reichl
161. Design of an Animal Protein Free Medium to Sustain Vero Cells Growth in a Stirred Tank Bioreactor
H. Kallel, S. Rourou, A. Ark, M. Thalen, T. Velden De Groot
162. Use of On-Line Our Measurements to Monitor and Improve Insect Cell Cultures for Recombinant Protein Production
M. Lecina, A. Casablancas, A. Soley, C. Vela, E. Espuña, J. Gràcia, J.J. Cairó, F. Gòdia
163. Production of Live Influenza Vaccine in MDCK Cell Line
N. Mazurkova, E. Nechaeva, T. Ryabicheva, N. Varaksin, T. Sen'Kina, T. Sviridenko, T. Kolokoltsova, S. Markushin, Yu. Gendon
164. Elaboration of the Microencapsulated Forms of the Viral Vaccines
E. Nechaeva, N. Varaksin, T. Ryabicheva, T. Getmanova, N. Mazurkova, T. Senkina, T. Kolokoltsova
165. HTS-Based Development of High-Producing CHO Cell Lines
K. Sautter, J. Fieder, R. Otto, B. Enenkel
DRM-restrictions
Printing: not available
Clipboard copying: not available
Keywords: SCIENCE / Life Sciences / Biology / General SCI008000
- Author(s)
- Fussenegger, Martin
- Gòdia, Francesc
- Publisher
- Springer
- Publication year
- 2005
- Language
- en
- Edition
- 1
- Category
- Natural Sciences
- Format
- Ebook
- eISBN (PDF)
- 9781402031038